Figure 3: Analysis of EMT markers. (a) qRT-PCR analysis of EMT markers in 22Rv1 cells with ZEB1 overexpression and DU145 cells with ZEB1 silencing. (b) Western Blot analysis of EMT markers in 22Rv1 cells overexpressing ZEB1 and DU145 with ZEB1 silencing. (c) Quantification of the Western blot results. Each image shows a representative experiment repeated three times with similar results (n = 3). *P < 0.05 in all cases. qRT-PCR: quantitative reverse transcription-polymerase chain reaction; EMT: epithelial–mesenchymal transition.