Figure 1: ZEB1 expression in human prostate cancer samples, cell lines, and basal levels of EMT markers in cell lines. (a) Representative pictures of immunohistochemical staining for ZEB1 in different samples of low (≤6) and high (≥8) Gleason score (×40). Scale bars = 50 μm. (b) Summary of the differential expression of ZEB1 between samples of low and high Gleason score. (c) Western blot analysis of ZEB1 and EMT markers in different prostate cancer cell lines. (d) Graphical representation of the Western blot results. All experiments were carried out three times with similar results (n = 3). *P < 0.05 in all cases. EMT: epithelial–mesenchymal transition.