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  Indian J Med Microbiol
 

Figure 1: Box-plots comparing the relative mRNA expression of the PIWI-LIKE 1 and PIWI-LIKE 2 (expressed in Δ CT values) in ejaculated spermatozoa stratified to total sperm count and progressive motility. While (a) PIWI-LIKE 1 expression was not associated with sperm count, (b) samples from patients with oligozoospermia (<39 × 106 spermatozoa per ejaculate; n = 26) exhibited a decreased PIWI-LIKE 2 mRNA expression in comparison with normozoospermia (≥39 × 106 spermatozoa per ejaculate; n = 57, P = 0.022, Mann–Whitney U-test). (c) Samples from patients with asthenozoospermia (<32%; n = 23) exhibited an increased PIWI-LIKE 1 mRNA expression compared with normozoospermic patients (≥32%; n = 60; P = 0.048, Mann–Whitney U-test), while (d) PIWI-LIKE 2 mRNA expression was not associated with motility. Note the different y-axis scales indicating the higher expression of PIWI-LIKE 1 mRNA in comparison to PIWI-LIKE 2 mRNA. The boxes designate the distribution of 50% of the measured values; the lines designate the median value. The whiskers define the 2.5% and 97.5% quantiles.

Figure 1: Box-plots comparing the relative mRNA expression of the <i>PIWI-LIKE 1</i> and <i>PIWI-LIKE 2</i> (expressed in Δ C<sub>T</sub> values) in ejaculated spermatozoa stratified to total sperm count and progressive motility. While (<b>a</b>) <i>PIWI-LIKE 1</i> expression was not associated with sperm count, (<b>b</b>) samples from patients with oligozoospermia (<39 × 10<sup>6</sup> spermatozoa per ejaculate; <i>n</i> = 26) exhibited a decreased <i>PIWI-LIKE 2</i> mRNA expression in comparison with normozoospermia (≥39 × 10<sup>6</sup> spermatozoa per ejaculate; <i>n</i> = 57, <i>P</i> = 0.022, Mann–Whitney <i>U</i>-test). (<b>c</b>) Samples from patients with asthenozoospermia (<32%; <i>n</i> = 23) exhibited an increased <i>PIWI-LIKE 1</i> mRNA expression compared with normozoospermic patients (≥32%; <i>n</i> = 60; <i>P</i> = 0.048, Mann–Whitney <i>U</i>-test), while (<b>d</b>) <i>PIWI-LIKE 2</i> mRNA expression was not associated with motility. Note the different y-axis scales indicating the higher expression of <i>PIWI-LIKE 1</i> mRNA in comparison to <i>PIWI-LIKE 2</i> mRNA. The boxes designate the distribution of 50% of the measured values; the lines designate the median value. The whiskers define the 2.5% and 97.5% quantiles.