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  Indian J Med Microbiol
 

Figure 3: The role of p53 in Andro mediated apoptosis-enhancement. (a) The expression of p53 and DR4 in PC3 cells treated as indicated for 12 h. (b) The expression of p53 in PC3 cells transfected with control siRNA or p53 siRNA for 24 h. (c) The expression levels of p53 and DR4 in PC3 cells transfected with control siRNA or p53 siRNA for 24 h and then treated (or not treated) with Andro for 12 h. (d) The apoptosis of PC3 cells transfected with control siRNA or p53 siRNA for 24 h, and then treated with control or both TRAIL (20 ng ml−1) and Andro (20 µmol l−1) for another 24 h. si-p53 vs NC, ***P < 0.05. Andro: andrographolide; TRAIL: tumor necrosis factor-related apoptosis-inducing ligand; NC: negative control for p53 siRNA.

Figure 3: The role of p53 in Andro mediated apoptosis-enhancement. (a) The expression of p53 and DR4 in PC3 cells treated as indicated for 12 h. (b) The expression of p53 in PC3 cells transfected with control siRNA or p53 siRNA for 24 h. (c) The expression levels of p53 and DR4 in PC3 cells transfected with control siRNA or p53 siRNA for 24 h and then treated (or not treated) with Andro for 12 h. (d) The apoptosis of PC3 cells transfected with control siRNA or p53 siRNA for 24 h, and then treated with control or both TRAIL (20 ng ml−1) and Andro (20 µmol l−1) for another 24 h. si-p53 <i>vs</i> NC, ***<i>P</i> < 0.05. Andro: andrographolide; TRAIL: tumor necrosis factor-related apoptosis-inducing ligand; NC: negative control for p53 siRNA.