Figure 1: Effects of Andro and TRAIL on the apoptosis in PCa cells and normal cells. (a) The apoptosis in PCa cell lines (PC3, DU145, JCA-1, TsuPr1 and LNCaP) and 293T under different concentrations (0 µmol l−1, 10 µmol l−1, 20 µmol l−1, 30 µmol l−1) of Andro treatment with or without TRAIL (20 ng ml−1) for 24 h. TRAIL vs TRAIL (0), ***P < 0.05. The apoptosis of (b) PC3, (c) DU145, (d) JCA-1, (e) TsuPr1 and (f) LNCaP treated with 20 µmol l−1 Andro, 20 ng ml−1 TRAIL, or both for 0, 8, 12, 16, 24 and 30 h, and untreated cells that comprised as the blank control group. (g) Effects of Andro and TRAIL on the apoptosis of PCa cells and normal cells. PC3, normal prostate epithelial cell line RWPE-1, human prostate stromal cells PS30, human hepatocytes HL7702 and 293T cells were treated with 30 µmol l−1 Andro, 20 ng ml−1 TRAIL or both for 24 h, and untreated cells comprised the blank control group. TRAIL + Andro vs control or TRAIL or Andro, ***P < 0.05. Andro: andrographolide; TRAIL: tumor necrosis factor-related apoptosis-inducing ligand; ns: not significant.