Figure 4: Knockdown of PGAM1 inhibited prostate cancer cell migration and invasion. (a) Transwell migration and transwell invasion assays showed that the cell numbers were markedly decreased in si-PGAM1 transfected cells (scale bars = 50 μm). (b) Statistics analysis of the mean migration and invasion cell numbers as compared with the negative control (n = 3). All experiments were performed three times independently. (c) Western blotting shown that PGAM1 knockdown decreased the expression of MMP-2 and MMP-9. **P < 0.01; ***P < 0.001. NC: negative controls; PGAM1: phosphoglycerate mutase 1. MMP: matrix metallopeptidase.