Figure 3: Enhanced cell apoptosis rate by downregulation of PGAM1. (a) PGAM1 knockdown increased the rate of apoptosis by flow cytometry. (b) Statistics analysis of the apoptosis rate in each group (n = 3, Student's t-test). (c) Western blot shown that PGAM1 knockdown increased the expression of cleaved caspase-3 and Bax, whereas the expression of Bcl-2 was decreased. **P < 0.01; ***P < 0.001. NC: negative controls; PGAM1: phosphoglycerate mutase 1; FITC-A: Fluorescein isothiocyanate-Annexin V.