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  Indian J Med Microbiol
 

Figure 1: PGAM1 expression in prostate cancer tissues and prostate cancer cell lines. (a) The immunohistochemistry image of the whole TMA (scale bar = 1.5 mm). Representative immunohistochemistry images of PGAM1 protein expression from (a) with high intensity (b) in prostate cancer tissue (D7, scale bar = 0.2 mm), with intermediate intensity (c) in prostate cancer tissue (H7, scale bar = 0.2 mm), and with low intensity (d) in prostate cancer tissue (E4, scale bar = 0.2 mm). The percentage of PGAM1 positive cells in D7 is 90%, in H7 is 70%, and in E4 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 × 1 = 1. (e) Part of C14 from (a) with a magnification of 400× (scale bar = 50 μm). (f) Western blotting of PGAM1 protein expression in prostate cancer cell lines and normal prostate epithelial cell lines. PGAM1: phosphoglycerate mutase 1; TMA: tissue microarray.

Figure 1: PGAM1 expression in prostate cancer tissues and prostate cancer cell lines. (a) The immunohistochemistry image of the whole TMA (scale bar = 1.5 mm). Representative immunohistochemistry images of PGAM1 protein expression from (a) with high intensity (b) in prostate cancer tissue (D7, scale bar = 0.2 mm), with intermediate intensity (c) in prostate cancer tissue (H7, scale bar = 0.2 mm), and with low intensity (d) in prostate cancer tissue (E4, scale bar = 0.2 mm). The percentage of PGAM1 positive cells in D7 is 90%, in H7 is 70%, and in E4 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 × 1 = 1. (e) Part of C14 from (a) with a magnification of 400× (scale bar = 50 μm). (f) Western blotting of PGAM1 protein expression in prostate cancer cell lines and normal prostate epithelial cell lines. PGAM1: phosphoglycerate mutase 1; TMA: tissue microarray.