Figure 3: Identification of AMPK protein in human sperm by Western blotting. (a) Human sperm proteins (10 μg) from different donors were analyzed by Western blot using anti-AMPKα1/2 as the primary antibody. (b) Different patterns of AMPK cross-reacted bands in several donors of human sperm. Arrows indicate cross-reactive bands of AMPK protein recognized by the antibody. Molecular weight markers are indicated in KDa. Loading control was performed for each experiment in the same membranes using the anti-α tubulin antibody and is shown on the lower panels. Each experiment was performed seven times and representative films are shown.