Figure 5: Effect of the AMPK inhibition by compound C (CC) for 20 h in human sperm motility parameters. After isolation by density gradient human sperm were incubated at 37°C in the absence (unfilled bars) or presence (filled bars) of AMPK inhibitor compound C (CC 30 μmol l−1) during 20 h. Following sperm motility parameters were evaluated by the ISAS system: (a) Motile spermatozoa, expressed as % of total spermatozoa, (b) curvilinear velocity, (c) average velocity and (d) straight-line velocity expressed as μm s−1. This experiment was performed at least six times and results express the mean ± standard error of the mean. Statistical differences were considered when P < 0.05: histograms with different letters (a-c) are statistically different from each other. ISAS: integrated semen analysis system.