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  Indian J Med Microbiol
 

Figure 2: Vasectomy induces changes in mRNA and protein levels of HSP110 family members. (a) The level of HSP110 mRNA in the mouse testis was determined by RT-PCR. (b) Quantification of ORP150 and HSPA4l mRNA in vasectomized and control mice relative to β-actin. Control, one representative of sham-operated control (note that sham-operated controls at each time point postvasectomy had the same expression level for each HSP110 gene). *Statistically significant difference for vasectomy versus sham operation of the same day (P < 0.05). Results of one representative experiment of two others. (c) Expression of HSP110 in mouse testes at different time points postvasectomy detected by Western blot. GAPDH was assessed as an internal loading control. Control, one representative of sham-operated control (note that sham-operated controls at each time point postvasectomy had the same expression level for each HSP110 protein). Results of one representative experiment of two others.

Figure 2: Vasectomy induces changes in mRNA and protein levels of HSP110 family members. (a) The level of HSP110 mRNA in the mouse testis was determined by RT-PCR. (b) Quantification of ORP150 and HSPA4l mRNA in vasectomized and control mice relative to β-actin. Control, one representative of sham-operated control (note that sham-operated controls at each time point postvasectomy had the same expression level for each HSP110 gene). *Statistically significant difference for vasectomy versus sham operation of the same day (<i>P</i> < 0.05). Results of one representative experiment of two others. (c) Expression of HSP110 in mouse testes at different time points postvasectomy detected by Western blot. GAPDH was assessed as an internal loading control. Control, one representative of sham-operated control (note that sham-operated controls at each time point postvasectomy had the same expression level for each HSP110 protein). Results of one representative experiment of two others.