Figure 2: Steps of sperm freezing with the LSL straw. (a) Prepare micro-drop of mixed semen and cryoprotectant in a culture dish. (b) Move up the mental cover of LSL straw. (c) Suction of mixed sample into the LSL straw using 1 ml syringe attached rubber tube. (d) Placed LSL straw to a freezing stent for fumigating with liquid nitrogen.