Close
  Indian J Med Microbiol
 

Figure 2: Pyrosequencing to evaluate repetitive-element class-specific methylation in rat spermatozoa. Pyrosequencing of CpG sites in three types of satellite repeats, LINE elements and IAP retrotransposon LTRs revealed the DNA methylation state of each in the spermatozoa of obese and control rats. Percentages displayed for each repetitive element type are from one CpG site in SATI, ISAT and 91ES8, an average of 7 CpG sites in LINE elements and an average of 3 CpG sites in the LTR of an IAP element. Data are mean ± s.d. SATI and 91ES8 Control n = 7, HFD n = 9; ISAT Control n = 8, HFD n = 8; LINE Control n = 8, HFD n = 9. IAP LTR Control n = 8, HFD n = 8

Figure 2: Pyrosequencing to evaluate repetitive-element class-specific methylation in rat spermatozoa. Pyrosequencing of CpG sites in three types of satellite repeats, LINE elements and IAP retrotransposon LTRs revealed the DNA methylation state of each in the spermatozoa of obese and control rats. Percentages displayed for each repetitive element type are from one CpG site in SATI, ISAT and 91ES8, an average of 7 CpG sites in LINE elements and an average of 3 CpG sites in the LTR of an IAP element. Data are mean ± s.d. SATI and 91ES8 Control <i>n</i> = 7, HFD <i>n</i> = 9; ISAT Control <i>n</i> = 8, HFD <i>n</i> = 8; LINE Control n = 8, HFD <i>n</i> = 9. IAP LTR Control <i>n</i> = 8, HFD <i>n</i> = 8