Figure 1: (a) Proteins from adult testes (T) or cauda epididymidal spermatozoa (S) immunoprecipitated by an anti-basigin antibody. T-1, T-2, S-1 and S-2 bands were cut from the gel and analyzed by LC-MS/MS. (b) Amino acid sequence of basigin. The peptides corresponding to basigin protein detection by LC-MS/MS spectra were analyzed using Mascot Server, and matched peptides are shown in red. 1-21: signal peptide, 24-103: Ig-like C2-type domain, 105-203: Ig-like V-type domain, 210-233: transmembrane, 234-273: cytoplasmic.