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  Indian J Med Microbiol
 

Figure 4: Effect of CNP on the human sperm acrosome reaction by the Coomassie brilliant blue staining method. (a) Data showing the percentage of acrosome-reacted cells at different incubation times under different concentrations of CNP (0, 10−7 and 10−6 mol l−1). (X̄ standard deviation, n = 10, *P < 0.05, **P < 0.01, compared with the control group). (b) The acrosome region is stained blue in the acrosome-intact sperm but unstained or stained light purple in the acrosome-reacted sperm, Original magnification 1000. CNP: C-type natriuretic peptide.

Figure 4: Effect of CNP on the human sperm acrosome reaction by the Coomassie brilliant blue staining method. (a) Data showing the percentage of acrosome-reacted cells at different incubation times under different concentrations of CNP (0, 10−7 and 10−6 mol l−1). (X̄  standard deviation, <i>n</i> = 10, *<i>P</i> < 0.05, **<i>P</i> < 0.01, compared with the control group). (b) The acrosome region is stained blue in the acrosome-intact sperm but unstained or stained light purple in the acrosome-reacted sperm, Original magnification 1000. CNP: C-type natriuretic peptide.