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  Indian J Med Microbiol
 

Figure 8: The influence of NODAL in human Sertoli cell proliferation and cell cycle proteins. (a): CCK-8 assay showed that NODAL promoted human Sertoli cell growth. OD values of Sertoli cells under 100 ng ml−1 human recombinant NODAL treatment were remarkably higher than those of other groups from 24 h to 72 h of culture. "*" indicates that 100 ng ml−1 human recombinant NODAL treatment displayed the highest OD values when compared with those in other groups at the same time points (P < 0.05). "#" indicates that OD values of each group at that time point was higher than that of the 24 h of culture. (b and c), Western blots reflected the effect of NODAL on the expression of CYCLIN A, CYCLIN D1, and CYCLIN E in human Sertoli cells from OA and SCO patients. Notes: In b and c, 1, 2, and 3 represented proteins changes of OA and SCO Sertoli cells with the control, NODAL treatment, and NODAL and SB431542 treatment, respectively, for 24 h; 4, 5, and 6 represented proteins changes of OA and SCO Sertoli cells with control, NODAL treatment, and NODAL and SB431542 treatment, respectively, for 48 h. OA: obstructive azoospermia; SCO: Sertoli cell-only syndrome.

Figure 8: The influence of NODAL in human Sertoli cell proliferation and cell cycle proteins. (a): CCK-8 assay showed that NODAL promoted human Sertoli cell growth. OD values of Sertoli cells under 100 ng ml−1 human recombinant NODAL treatment were remarkably higher than those of other groups from 24 h to 72 h of culture. P < 0.05). "#" indicates that OD values of each group at that time point was higher than that of the 24 h of culture. (b and c), Western blots reflected the effect of NODAL on the expression of CYCLIN A, CYCLIN D1, and CYCLIN E in human Sertoli cells from OA and SCO patients. Notes: In b and c, 1, 2, and 3 represented proteins changes of OA and SCO Sertoli cells with the control, NODAL treatment, and NODAL and SB431542 treatment, respectively, for 24 h; 4, 5, and 6 represented proteins changes of OA and SCO Sertoli cells with control, NODAL treatment, and NODAL and SB431542 treatment, respectively, for 48 h. OA: obstructive azoospermia; SCO: Sertoli cell-only syndrome.">