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  Indian J Med Microbiol
 

Figure 1: Primers for the GSTM1 gene were: 5′-CTGCCCTACTTGATTGATGGG-3′ and 5′-CTGGATTGTAGCAGATCATGC-3′; those for GSTT1 were: 5′-TTCCTTACTGGTCCTCACATCTC-3′ and 5′-TCACCGGATCATGGCCAGCA-3′; primers for human β-globin (110-bp amplicon), used as an internal control, were: 5′-ACACAACTGTGTTCACTAGC-3′ and 5′-CAACTTCATCCACGTTCACC-3′. All primers were manufactured by Sangon Biotech. ( a ) Detection of the GSM1 and GSTT1 alleles by multiplex polymerase chain reaction. M: 100 bp DNA ladder (Sangon Biotech, Shanghai, China); lanes 3, 4, 11, 12, 14: Even internal control is missing, these are to be retested; lane 5, 16: GSTM1 present and GSTT1 present; lanes 6, 10: GSTT1 present; lanes 1, 2, 7-9, 15: GSTM1 present; lane 13: GSTM1 null and GSTT1 null. ( b ) Detection of GSTP1 by restriction fragment length polymorphism. M: DL 1,000 DNA Marker (Takara Biotechnology Co., Ltd., Dalian, China); lanes 1, 5, 9, 11, 16-18, 23, 24: GSTP1 (Ile/Val); lanes 2-4, 6-8, 10, 13-15, 20, 22: GSTP1 (Ile/Ile); lanes 12, 19, 21: GSTP1 (Val/Val).

Figure 1: Primers for the <i>GSTM1</i> gene were: 5′-CTGCCCTACTTGATTGATGGG-3′ and 5′-CTGGATTGTAGCAGATCATGC-3′; those for <i>GSTT1</i> were: 5′-TTCCTTACTGGTCCTCACATCTC-3′ and 5′-TCACCGGATCATGGCCAGCA-3′; primers for human β-globin (110-bp amplicon), used as an internal control, were: 5′-ACACAACTGTGTTCACTAGC-3′ and 5′-CAACTTCATCCACGTTCACC-3′. All primers were manufactured by Sangon Biotech. ( a ) Detection of the <i>GSM1</i> and <i>GSTT1</i> alleles by multiplex polymerase chain reaction. M: 100 bp DNA ladder (Sangon Biotech, Shanghai, China); lanes 3, 4, 11, 12, 14: Even internal control is missing, these are to be retested; lane 5, 16: <i>GSTM1</i> present and <i>GSTT1</i> present; lanes 6, 10: <i>GSTT1</i> present; lanes 1, 2, 7-9, 15: <i>GSTM1</i> present; lane 13: <i>GSTM1</i> null and <i>GSTT1</i> null. ( b ) Detection of <i>GSTP1</i> by restriction fragment length polymorphism. M: DL 1,000 DNA Marker (Takara Biotechnology Co., Ltd., Dalian, China); lanes 1, 5, 9, 11, 16-18, 23, 24: <i>GSTP1</i> (Ile/Val); lanes 2-4, 6-8, 10, 13-15, 20, 22: <i>GSTP1</i> (Ile/Ile); lanes 12, 19, 21: <i>GSTP1</i> (Val/Val).