Figure 4: Effect of Panax ginseng on CatSper protein levels based on immunofluorescence with hematoxylin staining. CatSper1, 2, 3, and 4 levels are the results from normal (N) and P. ginseng-treated (100, 500 and 1000 mg kg−1 ) groups. CatSper proteins had a positive rounded form (a, d, g, j) in the mouse testes. All samples were counterstained with hematoxylin (b, e, h, k). Overlaid CatSper immunofluorescence and hematoxylin-counterstained images showing induction of CatSper proteins (c, f, i, l). In each column, a-c is the normal group and d-f, g-i and j-l are the P. ginseng-treated groups (100, 500 and 1000 mg kg−1 ), respectively. Images were obtained at a magnification of ×40. Scale bars = 50 μm.