Figure 3: siRNA-induced down-regulation of TIMELESS in PC3M cells reduces cell migration. ( a ) Treatment of PC3M cells with siTIMELESS leads to a marked reduction in TIMELESS protein levels, ( b ) but have no effect on cell proliferation. ( c ) A monolayer of PC3M cells was scratched to examine the rate of cell migration into the wounded area. The bar graph represents the percentage of cell-recovered wound areas after 24 h of incubation (*P < 0.01). Representative images of the wound captured at different time points are shown (at right). Results are representative of three individual experiments (mean ± S.D.). S.D., standard deviation.