Figure 1: Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) examination of SETDB1 expression in prostate cancer (PCa) tissues, adjacent normal tissues and cell lines. (a) SETDB1 was overexpressed in PCa tissue compared with adjacent normal tissues (n = 19, P = 0.008). Expression of SETDB1 is higher in paired normal tissues than in PCa tissue (n = 6, P = 0.011). (b) SETDB1 expression was significantly higher in PCa tissues than in benign prostate hyperplasia (BPH) tissues. For androgen-dependent PCa (ADPC) (n = 12) vs BPH, the average fold increase is 2.34 (P = 0.006), whereas, for androgen-independent PCa (AIPC) (n = 5) vs BPH, the increase is 6.21-fold (P < 0.001). AIPC vs ADPC is 1.78-fold higher (P = 0.001). (c) SETDB1 expression was higher in PCa cell lines (22RV-1, LNCaP-AI, LNCaP-AD, C4-2, DU145 and PC3) than in normal prostate epithelial cell lines (RWPE-1).