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  Indian J Med Microbiol
 

Figure 2: Effect of anti-inositol 1,4,5-trisphosphate type-1 receptor/BioPORTER® on progesterone-, zona pellucida-, and calcium ionophore-induced calcium influx. (a) Summary graph. The calcium influx curves in this figure represent the change in the average calcium concentration of the groups. Black arrows indicate when inducers were added. The raw intensity values were normalized as ΔF, ΔF = (F − Fbaseline)/Fbaseline. “F” represent the mean FITC fluorescence intensity of each gate and “Fbaseline” represents of the mean FITC fluorescence intensity of the initial 2 min. The data are expressed as the mean, n = 3. (b) Effect of anti-inositol 1,4,5-trisphosphate type-1 receptor/BioPORTER® on progesterone-induced calcium influx. The “*” represents this curve, and the versus curve treated with inducer only was significantly different. The data are expressed as the mean, n = 3. (c) Effect of anti-inositol 1,4,5-trisphosphate type-1 receptor/BioPORTER® on zona pellucida-induced calcium influx. The data are expressed as the mean, n = 3. (d) Effect of anti-inositol 1,4,5-trisphosphate type-1 receptor/BioPORTER® on calcium ionophore-induced calcium influx. The data are expressed as the mean, n = 3. (e) Description of the legends in a, b, c and d. Figure legends are given a series of two-letter groupings. The first letter indicates the pretreatment reagent, and the second letter represents the inducers. For the first letter, “C” indicates Earle's balanced salt solution supplemented with 0.0% BSA (EBSS [0.0% BSA]), and “O” represent anti-IP3R1/BioPORTER®. For the second letter, “P” indicates progesterone, “T” represents ZP, “A” indicates A23187 and “E” indicates EBSS (0.0% BSA). IP3R1: inositol 1,4,5-trisphosphate type-1 receptor; ZP: zona pellucida.

Figure 2: Effect of anti-inositol 1,4,5-trisphosphate type-1 receptor/BioPORTER<sup>®</sup> on progesterone-, zona pellucida-, and calcium ionophore-induced calcium influx. (a) Summary graph. The calcium influx curves in this figure represent the change in the average calcium concentration of the groups. Black arrows indicate when inducers were added. The raw intensity values were normalized as ΔF, ΔF = (F − F<sub>baseline</sub>)/F<sub>baseline</sub>. “F” represent the mean FITC fluorescence intensity of each gate and “F<sub>baseline</sub>” represents of the mean FITC fluorescence intensity of the initial 2 min. The data are expressed as the mean, <i>n</i> = 3. (b) Effect of anti-inositol 1,4,5-trisphosphate type-1 receptor/BioPORTER<sup>®</sup> on progesterone-induced calcium influx. The “<sup>*</sup>” represents this curve, and the versus curve treated with inducer only was significantly different. The data are expressed as the mean, <i>n</i> = 3. (c) Effect of anti-inositol 1,4,5-trisphosphate type-1 receptor/BioPORTER<sup>®</sup> on zona pellucida-induced calcium influx. The data are expressed as the mean, <i>n</i> = 3. (d) Effect of anti-inositol 1,4,5-trisphosphate type-1 receptor/BioPORTER<sup>®</sup> on calcium ionophore-induced calcium influx. The data are expressed as the mean, <i>n</i> = 3. (e) Description of the legends in a, b, c and d. Figure legends are given a series of two-letter groupings. The  first letter indicates the pretreatment reagent, and the second letter represents the inducers. For the  first letter, “C” indicates Earle's balanced salt solution supplemented with 0.0% BSA (EBSS [0.0% BSA]), and “O” represent anti-IP3R1/BioPORTER®. For the second letter, “P” indicates progesterone, “T” represents ZP, “A” indicates A23187 and “E” indicates EBSS (0.0% BSA). IP3R1: inositol 1,4,5-trisphosphate type-1 receptor; ZP: zona pellucida.