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  Indian J Med Microbiol
 

Figure 1: Determination of the optimal experimental conditions for BioPORTER®. (a) BioPORTER® transfer efficiency of anti-inositol 1,4,5-trisphosphate type-1 receptor at different treatment times. The FITC-labeled anti-IP3R1 was transferred into spermatozoa by BioPORTER®, and the transfer efficiency is represented as the FITC-positive spermatozoa. (b) BioPORTER® transfer efficiency of anti-inositol 1,4,5-trisphosphate type-1 receptor at different concentrations. The actual transfer efficiency is represented as the difference between the groups with or without BioPORTER® treatment. The data are expressed as the mean ± s.d., n = 3. (c) BioPORTER® transfer efficiency of inositol 1,4,5-trisphosphate in different proportions. The data are expressed as the mean ± s.d., n=3. (d) Fluorescent images of sperm. Scale bars = 50 μm. FITC: fluorescein isothiocyanate; IP3R1: inositol 1,4,5-trisphosphate type-1 receptor; s.d.: standard deviation.

Figure 1: Determination of the optimal experimental conditions for BioPORTER<sup>®</sup>. (a) BioPORTER<sup>®</sup> transfer efficiency of anti-inositol 1,4,5-trisphosphate type-1 receptor at different treatment times. The FITC-labeled anti-IP3R1 was transferred into spermatozoa by BioPORTER<sup>®</sup>, and the transfer efficiency is represented as the FITC-positive spermatozoa. (b) BioPORTER<sup>®</sup> transfer efficiency of anti-inositol 1,4,5-trisphosphate type-1 receptor at different concentrations. The actual transfer efficiency is represented as the difference between the groups with or without BioPORTER<sup>®</sup> treatment. The data are expressed as the mean ± s.d., <i>n</i> = 3. (c) BioPORTER<sup>®</sup> transfer efficiency of inositol 1,4,5-trisphosphate in different proportions. The data are expressed as the mean ± s.d., <i>n</i>=3. (d) Fluorescent images of sperm. Scale bars = 50 μm. FITC: fluorescein isothiocyanate; IP3R1: inositol 1,4,5-trisphosphate type-1 receptor; s.d.: standard deviation.