Figure 3: Ketotifen fumarate does not induce changes in cell mediated immunity in vivo. Rats with EAO treated with saline (vehicle) or ketotifen fumarate were tested for DTH to testicular antigens during (a) treatment 1 and (b) treatment 2. Two days before euthanasia (day 54), TH was injected into rats' left footpad. The right footpad was injected with saline. Footpad swelling was measured after 48 h and results are expressed as the mean difference between the thickness of the HT-injected footpad and that of the saline-injected footpad ± standard error of the mean (s.e.m.) in each group (nonparametric one-way ANOVA Kruskal–Wallis test). EAO: experimental autoimmune orchitis; DTH: delayed-type hypersensitivity.