|LETTER TO THE EDITOR
|Ahead of print publication
Chromosome heteromorphisms: do they entail a reproductive risk for male carriers?
Ester Anton1, Elena Garcia-Guixé2, Mireia Ramos-Muntada1, Anna Godo1, Mireia Sandalinas2, Joan Blanco1
1 Genetics of Male Fertility Group, Cell Biology Unit, Faculty of Biosciences, Autonomous University of Barcelona, Bellaterra (Cerdanyola del Vallès) 08193, Spain
2 Reprogenetics Spain, Barcelona 08006, Spain
|Date of Submission||05-Jun-2019|
|Date of Acceptance||29-Sep-2019|
|Date of Web Publication||10-Jan-2020|
Genetics of Male Fertility Group, Cell Biology Unit, Faculty of Biosciences, Autonomous University of Barcelona, Bellaterra (Cerdanyola del Vallès) 08193
Source of Support: None, Conflict of Interest: None
Article in PDF
|How to cite this URL:|
Anton E, Garcia-Guixé E, Ramos-Muntada M, Godo A, Sandalinas M, Blanco J. Chromosome heteromorphisms: do they entail a reproductive risk for male carriers?. Asian J Androl [Epub ahead of print] [cited 2020 Sep 24]. Available from: http://www.ajandrology.com/preprintarticle.asp?id=275506
Chromosome heteromorphisms are described as variations in size and morphology at specific regions that can be detected through classical banding methods. They are mitotically stable variants usually present in a heterozygous state (only one of the homologous chromosomes is heteromorphic). In humans, the most commonly detected heteromorphisms involve the heterochromatic regions of chromosomes 1, 9, 16, and Y (designated as 1qh, 9qh, 16qh, and Yqh, respectively), and the short-arms, satellites, or stalks of the acrocentric chromosomes 13, 14, 15, 21, and 22 (e.g., for chromosome 13 designated as 13p, 13ps, and 13pstk, respectively). Pericentric inversions involving the heterochromatic region of chromosomes 1, 9, and Y are also frequently observed.
Heteromorphisms are not associated with any phenotypic alteration. Nevertheless, several pieces of data suggest an association between the presence of heteromorphic variants and infertility. Moreover, the presence of a heteromorphism in one member of an infertile couple appears to have a detrimental effect on the outcome of assisted reproduction treatments and has been related to an increased frequency of miscarriage.
The underlying mechanisms behind the relationship between male infertility and the presence of such chromosomal variants are not fully understood. One of the current hypotheses has been related to a possible deleterious effect of the heteromorphisms on meiotic chromosome pairing and segregation. In this sense, some studies have shown that heteromorphisms in a heterozygous state can disturb homologous chromosome pairing during prophase I.,, This situation has been related to abnormal recombination that could promote chromosome missegregation during meiosis,, leading to higher frequencies of sperm aneuploidies.,,,
In this study, we provide additional information about the impact of chromosomal variants on the production of sperm chromosomal aneuploidies in carriers of different chromosome heteromorphisms.
Semen samples from 16 infertile heteromorphism carriers were obtained [Table 1]. Written informed consent was obtained from all carriers, and the study was approved by the Ethics Committee on Animal and Human Experimentation of the Autonomous University of Barcelona, Bellaterra (Cerdanyola del Vallès), Spain (reference number: 1883). Spermatozoa were spread on slides and processed for fluorescence in situ hybridization (FISH) following the four main steps of the methodology: sperm chromatin decondensation (5 mmol l−1 dithiothreitol solution, Merck, Darmstadt, Germany), DNA denaturation (70% formamide solution, Merck), probe and target DNA hybridization, and posthybridization washes (sodium citrate solution, Abbott Molecular Inc., Des Plaines, IL, USA). In each case, sperm diploidies and aneuploidies for chromosomes 1, 9, 13, 18, 21, 22, X, and Y were evaluated by performing three hybridizations in parallel with different combinations of probes (Abbott Molecular Inc., Des Plaines, IL, USA). The first probe combination included three Chromosome Enumeration Probes (CEP): CEP18 (D18Z1, Spectrum Aqua), CEPX (DXZ1, Spectrum Green), and CEPY (DYZ3, Spectrum Orange); the second probe combination included two Locus-Specific Identifier (LSI) probes: LSI 13 (13q14, Spectrum Green) and LSI 21 (21q22.13–21q22.2, Spectrum Orange); and probe combination three included Tel1q (VIJyRM2123, Spectrum Orange), CEP9 (9p11q11 alpha satellite, Spectrum Aqua), and LSI22 BCR (22q11.2, Spectrum Green). This broad panel was established to cover those chromosomes affected by heteromorphisms in the study population, as well as the most prone chromosomes to produce sperm with numerical imbalances in infertile patients. Sperm chromosome analysis was done using an Olympus BX60 epifluorescence microscope (Olympus Iberia, L'Hospitalet de Llobregat, Spain) equipped with filter sets for fluorescein isothiocyanate (FITC), Texas Red, Aqua, and 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)/Texas Red/FITC using standard assessment criteria. We analyzed 2000 spermatozoa per probe combination giving a total number of 6000 spermatozoa per individual. Sperm aneuploidy and diploidy data from each heteromorphic carrier were compared using a Fisher's exact test with internal cutoff values obtained from a control population constituted by six fertile individuals with normal karyotypes and normal seminal parameters [Table 1].
|Table 1: Individuals included in the study. In every case, karyotype, seminogram, age, and frequencies of chromosome anomalies detected are indicated|
Click here to view
Among the 16 heteromorphism carriers analyzed, 11 (68.8%) presented significantly increased rates of numerical abnormalities for at least one of the analyzed chromosomes [Table 1]. This result suggests the existence of a genetic reproductive risk associated with this population, supporting previously published data.,,, We would like to highlight the high heterogeneity regarding the chromosomes with significant aneuploidy increases among the carriers of the same chromosome heteromorphism. This is clearly observable in inv(9) carriers, which include cases without any significant increase (i.e., 940z) and carriers presenting significant increases affecting up to three chromosomes (i.e., 929z) [Table 1].
It is also noticeable that in 4 (36.4%) of the 11 individuals with increased frequencies of numerically abnormal sperm, one of the chromosomes involved in the aneuploidies was the heteromorphic chromosome itself (cases 923z, 911z, 931z, and 929z; [Table 1]. Similar to our results, a previous sperm-FISH study performed on a carrier of a heteromorphic chromosome 9 inversion detected increased frequencies of sperm with numerical abnormalities for several chromosomes also including the rearranged chromosome itself. All these data reinforce the hypothesis that in some cases, the establishment of heterosynapsis during prophase I might entail subsequent nondisjunction events at anaphase I that would affect both the segregation of the rearranged chromosomes and the segregation of other bivalents. This bidirectional phenomenon has been previously described in carriers of chromosomal translocations,, and this article extends its occurrence to heteromorphism carriers.
In the remaining 7 (63.6%) individuals with altered sperm FISH results in which the heteromorphic chromosome was unaffected by these increases, chromosomes 18 and 22 were the most frequent sperm anomalies detected [Table 1]. This finding argues in favor of the participation of other factors besides heterosynapsis in the production of such numerical anomalies. That is, in a scenario of meiotic disturbances produced by the heteromorphism with other unsynapsed regions, X and Y chromosomes (which contain large nonhomologous segments) would be among the most suitable candidates involved. Thus, a preferential increase in the incidence of sex chromosome aneuploidies should be expected. Nevertheless, only one individual (919z) showed increased incidences of sex chromosome aneuploidies.
Other factors besides heterosynapsis may also influence the frequency of chromosome imbalances in sperm. For example, abnormal seminal parameters have been generally associated with the presence of increased frequencies of sperm aneuploidies. Accordingly, one could say that the increased sperm aneuploidies observed in some cases would be the consequence of an abnormal seminogram, rather than a heterosynapsis phenomenon derived from the presence of a heteromorphic form. However, in our study, only two of the 11 individuals (i.e., 935z, 929z) that displayed increased frequencies of abnormal sperm had an altered seminogram [Table 1]. This low incidence of seminal anomalies among the carriers analyzed also agrees with other previous studies which indicated that the presence of heteromorphisms is not something directly related to the presence of altered seminal parameters.
Another factor that has been suggested to have a possible influence in the production of aneuploid/diploid sperm is paternal age. Up to present, controversial data have been obtained in this area with some studies reporting a correlation of age with certain types of numerical abnormalities while others do not observe such effect. In our study, individuals without altered FISH results ranged 32–39 years old, while individuals with increased frequencies of aneuploid/diploid sperm ranged 27–48 years old. The presence of such a broader age range in individuals with higher ratios of abnormal sperm does not support an effect of this factor on the production of sperm chromosomal abnormalities by heteromorphisms carriers.
Regardless of the origin of the observed sperm alterations, published data suggest a detrimental effect of heteromorphisms on assisted reproductive treatments,,, [20, Ultimately, this means that in reproductive counseling, significant differences in sperm chromosome anomaly rates should be taken into consideration. Accordingly, we suggest that a risk assessment through sperm FISH should be offered to these individuals, at least for cautionary purposes. Future studies examining larger populations of heteromorphism carriers would be of high interest to divide the individuals into “at-risk” and “without-risk” categories and clarify the effects of these variants over assisted reproductive treatments.
| Author Contributions|| |
EA, MS, and JB conceived, designed, and coordinated the study. EGG and MS were involved in sample collection. MRM and AG carried out the sperm FISH analysis. EA, EGG, MRM, AG, MS, and JB were involved in data analysis and interpretation; EA and JB wrote the manuscript with support from EGG and MS. All authors provided critical feedback, read, and approved the final manuscript.
| Competing Interests|| |
All authors declared no competing interests.
| Acknowledgments|| |
This work was supported by the 2017/SGR-503 project (Agency for Management of University and Research Grants, Spain) and UAB CF-180034 grant (Autonomous University of Barcelona).
| References|| |
Gardner R, Amor DJ. Chromosome Abnormalities and Genetic Counseling. 5th
ed. New York: Oxford University Press; 2018.
Liehr T. Cytogenetically visible copy number variations (CG-CNVs) in banding and molecular cytogenetics of human; about heteromorphisms and euchromatic variants. Mol Cytogenet
2016; 9: 5.
Tempest HG, Simpson JL. Why are we still talking about chromosomal heteromorphisms? Reprod Biomed Online
2017; 35: 1–2.
Solari AJ, Ponzio R, Rey Valzacchi G. Synaptonemal complex karyotyping in an oligospermic patient with heterochromatin duplication in chromosome n. 9. Medicina (B Aires)
1991; 51: 217–21.
Garcia-Peiro A, Oliver-Bonet M, Navarro J, Abad C, Guitart M, et al
. Sperm DNA integrity and meiotic behavior assessment in an infertile male carrier of a 9qh+++ polymorphism. J Biomed Biotechnol
2011; 2011: 730847.
Codina-Pascual M, Navarro J, Oliver-Bonet M, Kraus J, Speicher MR, et al
. Behaviour of human heterochromatic regions during the synapsis of homologous chromosomes. Hum Reprod
2006; 21: 1490–7.
Morales R, Lledo B, Ortiz JA, Ten J, Llacer J, et al
. Chromosomal polymorphic variants increase aneuploidies in male gametes and embryos. Syst Biol Reprod Med
2016; 62: 317–24.
Yakin K, Balaban B, Urman B. Is there a possible correlation between chromosomal variants and spermatogenesis? Int J Urol
2005; 12: 984–9.
Collodel G, Moretti E, Capitani S, Piomboni P, Anichini C, et al
. TEM, FISH and molecular studies in infertile men with pericentric inversion of chromosome 9. Andrologia
2006; 38: 122–7.
Amiel A, Sardos-Albertini F, Fejgin MD, Sharony R, Diukman R, et al
. Interchromosomal effect leading to an increase in aneuploidy in sperm nuclei in a man heterozygous for pericentric inversion (inv9) and C-heterochromatin. J Hum Genet
2001; 46: 245–50.
Sarrate Z, Anton E. Fluorescence in situ
hybridization (FISH) protocol in human sperm. J Vis Exp
2009; 1: 1405.
Sarrate Z, Vidal F, Blanco J. Role of sperm fluorescent in situ
hybridization studies in infertile patients: indications, study approach, and clinical relevance. Fertil Steril
2010; 93: 1892–902.
Blanco J, Egozcue J, Vidal F. Incidence of chromosome 21 disomy in human spermatozoa as determined by fluorescent in-situ
hybridization. Hum Reprod
1996; 11: 722–6.
Godo A, Blanco J, Vidal F, Anton E. Accumulation of numerical and structural chromosome imbalances in spermatozoa from reciprocal translocation carriers. Hum Reprod
2013; 28: 840–9.
Godo A, Blanco J, Vidal F, Sandalinas M, Garcia-Guixe E, et al
. Altered segregation pattern and numerical chromosome abnormalities interrelate in spermatozoa from Robertsonian translocation carriers. Reprod Biomed Online
2015; 31: 79–88.
Burgoyne PS. Genetic homology and crossing over in the X and Y chromosomes of Mammals. Hum Genet
1982; 61: 85–90.
Fonseka KG, Griffin DK. Is there a paternal age effect for aneuploidy? Cytogenet Genome Res
2011; 133: 280–91.
Guo T, Qin Y, Gao X, Chen H, Li G, et al
. The role of male chromosomal polymorphism played in spermatogenesis and the outcome of IVF/ICSI-ET treatment. Int J Androl
2012; 35: 802–9.
Xiao Z, Zhou X, Xu W, Yang J. A preliminary study of the relationship between the long arm of the Y chromosome (Yqh+) and reproductive outcomes in IVF/ICSI-ET. Eur J Obstet Gynecol Reprod Biol
2012; 165: 57–60.
Xu X, Zhang R, Wang W, Liu H, Liu L, et al
. The effect of chromosomal polymorphisms on the outcomes of fresh IVF/ICSI-ET cycles in a Chinese population. J Assist Reprod Genet
2016; 33: 1481–6.
Ni T, Li J, Chen H, Gao Y, Gao X, et al
. Male chromosomal polymorphisms
reduce cumulative live birth rate for IVF couples. J Assist Reprod Genet
2017; 34: 1017–25.