ORIGINAL ARTICLE
Ahead of print publication  

Influence of in vitro capacitation time on structural and functional human sperm parameters


1 Department of Biotechnology, Faculty of Science, University of Alicante, Alicante 03080, Spain
2 FISABIO - University Hospital of San Juan de Alicante, Service of Gynecology and Obstetrics, San Juan de Alicante 03550, Spain
3 Department of Cell Biology and Histology, Faculty of Medicine, University of Murcia and IMIB-Arrixaca, Murcia 30100, Spain
4 IVF Spain, Reproductive Medicine, Alicante 03540, Spain
5 University Hospital of San Juan de Alicante, Service of Gynecology and Obstetrics, San Juan de Alicante 03550, Spain
6 Human Fertility Cathedra, University of Alicante, Alicante 03080, Spain

Date of Submission06-Mar-2019
Date of Acceptance04-Jul-2019
Date of Web Publication15-Oct-2019

Correspondence Address:
María José Gómez-Torres,
Department of Biotechnology, Faculty of Science, University of Alicante, Alicante 03080; Human Fertility Cathedra, University of Alicante, Alicante 03080
Spain
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/aja.aja_104_19

PMID: 31621655

  Abstract 


A cascade of dramatic physiological events is linked to the sperm acrosome reaction and binding to the oocyte's zona pellucida during human sperm capacitation. However, structural and functional sperm changes during capacitation currently remain poorly defined. Here, we performed a multibiomarker approach based on the utilization of sperm concentration, motility, viability, morphology, acrosome reaction, tyrosine phosphorylation, DNA fragmentation, and lectin-binding sites to analyze the impact caused by swim-up selection times (uncapacitated, 1 h capacitated, and 4 h capacitated) on sperm function and structure in normozoospermic samples. We found that a 4 h swim-up capacitation increased sperm quality, because a large number of cells with normal morphology and lower DNA fragmentation rates were recovered. Furthermore, the long-term capacitation induced a higher percentage of cells with tyrosine phosphorylation of the principal piece as well as a redistribution of lectin-binding sites. Overall, the multivariate biomarkers analyzed showed a less variable distribution on spermatozoa recovered after 4 h capacitation than that with the shorter capacitation time. These findings stress the importance of capacitation time as a relevant factor in sperm quality with potential biological reproductive implications both for basic research and in assisted reproduction techniques.

Keywords: acrosome reaction; capacitation; DNA fragmentation; glycocalyx; lectin-binding sites; membrane integrity; protein phosphorylation; sperm selection


Article in PDF

How to cite this URL:
Sez-Espinosa P, Huerta-Retamal N, Robles-Gómez L, Avilés M, Aizpurua J, Velasco I, Romero A, Gómez-Torres MJ. Influence of in vitro capacitation time on structural and functional human sperm parameters. Asian J Androl [Epub ahead of print] [cited 2020 Jul 14]. Available from: http://www.ajandrology.com/preprintarticle.asp?id=269238




  Introduction Top


The success of mammalian fertilization depends largely on the spermatozoon's ability to acquire functionality through the female reproductive tract.[1] This process, known as capacitation,[2],[3],[4] refers to biochemical and physiological changes permitting the acrosome reaction and interaction with the oocyte.[5],[6] Early reports showed that capacitation can also be achieved in vitro in several species,[7] including humans.[8]

Examples of the most outstanding changes in human sperm capacitation are the efflux of membrane cholesterol;[9] the redistribution of glycoconjugates;[10] the entry of calcium into the cytoplasm followed by the increase of tyrosine phosphorylation;[11] and the acquisition of hyperactivated motility.[12] This cascade of changes in biomarkers associated with sperm capacitation has recently been outlined.[13],[14] Nevertheless, in vitro capacitation time in human spermatozoa covers an extensive range from 3 h to 24 h.[15] In this context, a study has reported that spermatozoa need to undergo capacitation in vitro for at least 4 h in order to recognize the oocyte's zona pellucida.[16] Wider capacitation ranges have been linked to the vast heterogeneity of semen samples, which result in sperm subpopulations with varying degrees of functionality and membrane cholesterol content.[17],[18] Finally, other reports have shown that capacitation timing in human spermatozoa differs among men despite being reproducible within each man.[19]

Standard semen analysis results (e.g., sperm concentration, motility, morphology, and viability) have limited predictive power for fertilization success.[20],[21],[22] Thus, new potential sperm biomarkers have been developed. These include the acrosome reaction, DNA damage, and oocyte's binding molecules.[23],[24],[25] The World Health Organization (WHO) stipulates times for conventional sperm selection protocols with a capacitating medium (for selection and capacitation), 1 h for swim-up and 15–30 min for discontinuous density gradients.[26] Therefore, studies on the influence of preparation time on sperm biomarkers only differ in longer incubation time after selection in the capacitation medium.[27],[28],[29] Optimal incubation times have been obtained for tyrosine phosphorylation,[27] DNA fragmentation,[28] and the acrosome reaction,[29] among others. However, studies that have considered sperm biomarkers after a longer combined sperm selection/capacitation time are particularly scarce.[30] This paper shows the first application of a novel multivariate approach to analyze the impact that different swim-up selection times with a capacitating medium (1 h and 4 h) have on quality and structural sperm biomarkers.


  Materials and Methods Top


Experimental design

Semen samples were obtained from 15 normozoospermic donors with their written informed consent. The research was approved by the Ethical Committee of the University of Alicante (Alicante, Spain) based on the Declaration of Helsinki principles. Each sperm sample was divided to obtain the three selected physiological conditions: uncapacitated (T0), 1 h selected under capacitating conditions (T1), and 4 h selected under capacitating conditions (T4). T1 was chosen in accordance with the WHO swim-up protocol [26] and T4 was based on previous protocols.[16] On the basis of these different physiological conditions, we examined a set of biomarkers, namely, basic sperm parameters, tyrosine phosphorylation, spontaneous and induced acrosome reactions, lectin-binding sites, and DNA fragmentation.

Preparation of semen samples

Donor semen samples were obtained by masturbation after 3–4 days of abstinence and subsequently analyzed in the laboratory of the Department of Biotechnology at the University of Alicante. The samples were allowed to liquefy for 15 min at room temperature, and basic seminogram analysis was performed by following the WHO guidelines.[26] Sperm concentration and motility assessment was undertaken using a Makler® (BioCare Europe, Rome, Italy) counting chamber; Papanicolaou staining (Panreac Química S.L.U., Barcelona, Spain) served for the evaluation of morphology, and vitality was studied by eosin–nigrosin staining (Projectes i Serveis R+D S.L., Paterna, Spain).

Sperm capacitation

Semen aliquots were selected/capacitated by swim-up in human tubal fluid medium (HTF, Origio®, Måløv, Denmark) supplemented with 5 mg ml −1 bovine serum albumin (BSA, Sigma-Aldrich®, Saint Louis, MO, USA) at 37°C and 5% (v/v) CO2 for 1 h (T1) and 4 h (T4). The supernatant fraction was subsequently collected and washed three times for 5 min in phosphate-buffered saline without calcium or magnesium, pH 7.4 (PBS, Biowest, Nuaillé, France) by centrifugation (250g, 10 min). Concentration, motility, morphology, and viability for recovery of each mobile spermatozoon (REM) were estimated. Cells from the different times considered (T0, T1, and T4) were fixed in 2% (w/v) paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 45 min at 4°C, after which paraformaldehyde was replaced with PBS and the samples were stored at 4°C.

Assessment of spontaneous and inducted acrosomal reaction

An aliquot of capacitated cells was induced with 0.01 mmol l −1 of calcium ionophore A23187 (Sigma-Aldrich) and 2 mmol l −1 calcium chloride (Panreac Química S.L.U., Barcelona, Spain) for 1 h at 37°C and 5% (v/v) CO2 following previous protocols.[31] Only calcium chloride was added to the controls for the purpose of detecting spontaneous acrosomal reaction. We also evaluated the percentage of spontaneously reacted cells in the T0 condition.

A total of 5 μl sperm suspension from each physiological condition was placed on coverslips and fixed in methanol for 30 min for the assessment of acrosomal status. After drying the smear, the cells were washed three times in PBS and unspecific binding was blocked by 2% (w/v) BSA-PBS for 30 min. The smears were then incubated in the dark with Pisum sativum lectin conjugated to fluorescein-5-isothiocyanate (PSA-FITC, Sigma-Aldrich) 50 μg ml −1 for 30 min. After three washes in PBS, the samples were mounted with Vectashield® and 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI, Vector Laboratories, Burlingame, CA, USA). DAPI helped with identification of the cell nucleus [Figure 1]a. The whole process took place at room temperature.
Figure 1: Sperm biomarker fluorescence. (a) PSA-label. Spermatozoa that fluoresced in the acrosomal region were considered not reacted (right) and those with labeling of the equatorial band were deemed as reacted (left). (b) PY20 fluorescent patterns. The equatorial segment, the principal piece, and the combined principal piece plus equatorial segment. (c) TUNEL assay. Green staining indicated DNA fragmentation. The sperm nucleus was detected by means of DAPI (blue). Scale bars = 20 μm. PSA: Pisum sativum lectin; TUNEL: terminal-deoxynucleotidyl transferase-mediated nick end labeling; DAPI: 4',6-diamidino-2-phenylindole.

Click here to view


Immunolocalization of tyrosine phosphorylation

A total of 5 μl of sperm suspension from each paraformaldehyde-fixed condition was deposited on a coverslip. When dry, the cells were washed three times in PBS and permeabilized by incubation in 0.1% (v/v) Triton X-100 (Sigma-Aldrich) for 10 min. In an attempt to prevent unspecific binding, spermatozoa were blocked with 2% (w/v) BSA-PBS for 30 min. Tyrosine phosphorylation was detected using an anti-phosphotyrosine primary antibody produced in mice (PY20, Sigma-Aldrich) at a 1:500 dilution for 1 h and a secondary anti-mouse IgG (H + L) antibody conjugated to Cyanine ™3 (Jackson ImmunoResearch, Ely, UK) at a 1:300 dilution for 1 h in the dark, taking previous protocols as reference.[11] Slides were rinsed with PBS between both incubations. Finally, coverslips were washed again three times with PBS and subsequently mounted with Vectashield and DAPI.

Assessment of DNA fragmentation

A terminal-deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit: Fluorescein according to the manufacturer's guidelines (Roche Diagnostics GmbH, Mannheim, Germany). A total of 5 μl fixed cell suspension from each experimental group (T0, T1, and T4) was first deposited on the slides and then rinsed in PBS and permeabilized with 0.2% (v/v) Triton X-100 for 5 min. The TdT-labeled nucleotide mixture was added and incubated at 37°C for 1 h in a dark, humid chamber, after which the slides were washed three times in PBS and mounted with Vectashield with DAPI.

Lectin cytochemistry

As lectins bind to the carbohydrate group of glycoconjugates, we used several lectins to characterize the spermatozoa surface carbohydrate distribution by means of cytofluorescence. More specifically, four lectinswereconjugated with FITC (Vector Laboratories): Aleuria aurantia agglutinin (AAA), Concanavalin A (ConA), Peanut agglutinin (PNA), and Wheat germ agglutinin (WGA). Each lectin presents a high specificity for a different carbohydrate. AAA recognizes fucose residues;[32] ConA has a high affinity for mannose;[33] PNA for galactose;[34] and WGA for sialic acid.[35] A total of 5 μl sperm suspension from each paraformaldehyde-fixed condition was deposited on a coverslip. Once dry, the smear was rehydrated three times with PBS and incubated in a 2% (w/v) BSA-PBS block solution for 1 h. The cells were subsequently incubated with each FITC-conjugated lectin at a final concentration of 20 μg ml −1 for 1 h, at room temperature, and in a dark, humid chamber as in previous studies.[36] Finally, spermatozoa were rinsed three times in PBS and mounted with Vectashield with DAPI.

The data related to the acrosome reaction, tyrosine phosphorylation, the TUNEL assay, and lectin-binding sites were examined using Leica TCS-SP2 confocal microscopy (Leica Microsystems GmBH, Wetzlar, Germany), and Leica Confocal Software helped obtain (1024 × 1024 pixel) images. At least 200 cells were evaluated for each biomarker and physiological condition (T0, T1, and T4). Added to this, the appropriate negative controls served to corroborate the specificity of each biomarker.

Statistical analyses

The Shapiro–Wilk test performed with the aim of testing the distribution and equal variance in the biomarkers under study showed that 81.5% of the biomarkers did not have a normal distribution (W = 0.540–0.946; P < 0.05). The nonparametric Kruskal–Wallis test was used to assess differences on the effect of uncapacitated (T0) and 1–4 h (T1–T4) capacitated sperm samples within each biomarker. A principal component analysis (PCA) on the variance–covariance matrix allowed calculation of individual specific biomarker patterns that accounted for most of the variability observed among uncapacitated and capacitated conditions. Descriptive and statistical results were obtained by means of IBM SPSS Statistics 19.0 (IBM, Armonk, NY, USA) and SYSTAT® 11 (Systat Software, Inc., Point Richmond, CA, USA). Two-sided P < 0.05 was deemed statistically significant.


  Results Top


Effects of capacitation time on sperm parameters

A significant decrease in sperm concentration (P < 0.001) along with an increase in motility and vitality (P < 0.001) became visible after both capacitation times (T1 and T4), compared with uncapacitated cells [Table 1]. The results showed an increase up to 26.6% in normal forms (P < 0.001) after 4 h capacitation compared with T0. No statistical significance corresponded to normal sperm morphology differences between T0 (9.7%) and T1 (19.3%).
Table 1: Descriptive and statistical results of sperm biomarkers in uncapacitated and swim-up capacitated conditions

Click here to view


Effects of capacitation time on spontaneous and induced acrosomal reactions

The spermatozoa that fluoresced in the acrosomal region were regarded as not having reacted. Those with a label in the equatorial segment were considered reacted [Figure 1]a. We did not find any differences after the induction of acrosome reaction using the calcium ionophore either at T1 or at T4 (P > 0.05). Furthermore, a high percentage of spontaneously acrosome-reacted cells was identified at T4 compared with T0 (P = 0.028) together with a lack of significant differences between T1 and T4 [Table 1].

Effects of capacitation time on sperm tyrosine phosphorylation

We defined three fluorescent tyrosine phosphorylation patterns, located at the equatorial segment (ES), the principal piece (PP), and the combined equatorial segment plus principal piece (ES + PP) [Figure 1]b. We generally found a significant growing percentage (P < 0.05) of cells with tyrosine phosphorylation at every sperm location (ES, PP, and ES + PP) when a comparison was made between capacitation times (T1 and T4) and T0. The most prominent increase between T1 and T4 corresponded to phosphorylation of the principal piece, which showed a significant increase from 17.2% to 25.3% (P = 0.004) [Table 1].

Effects of capacitation time on DNA sperm fragmentation

Apoptotic cells were recognized by means of positive green fluorescence [Figure 1]c. Fourteen percent of the analyzed cells showed DNA fragmentation at T0. The comparison of T0 with capacitating conditions (P < 0.05) revealed that the percentage of cells with fragmented DNA decreased to 3.3% (P = 0.077) after 1 h of capacitation and up to 2.0% after 4 h of capacitation (P = 0.047; [Table 1]).

Effects of capacitation time on plasma membrane sugar-binding sites

The findings revealed a high degree of carbohydrate surface heterogeneity, because sperm subpopulations with different location patterns appeared in all three physiological conditions (T0, T1, and T4). This allowed us to characterize five clearly defined patterns [Figure 2]: Pattern 1 (P1), characterized by a highly stained acrosomal region; Pattern 2 (P2), defined by a highly stained acrosomal and postacrosomal region with no labeling in the equatorial segment; Pattern 3 (P3), with labeling in the equatorial segment; Pattern 4 (P4), which showed a dotted label in the whole head; and Pattern 5 (P5), with a faint labeling in the whole head. Patterns 1 to 5 (P1–5) were quantified for both AAA and ConA lectins. However, only three patterns (P1, P3, and P5) emerged for the PNA lectin and four for WGA (P1, P3, P4, and P5).
Figure 2: Superposition of bright field and lectin-binding fluorescent patterns. (a) Pattern 1, highly stained acrosomal region. (b) Pattern 2, highly stained acrosomal and postacrosomal region. (c) Pattern 3, highly stained equatorial segment. (d) Pattern 4, dotted staining over the head. (e) Pattern 5, faint staining over the head. Scale bar = 10 μm.

Click here to view


Significant differences in the redistribution of glycoconjugates from T0 to T1 only became visible in the AAA and WGA-labeling patterns [Table 1]. We detected a significant increase (P < 0.001) in AAA lectin-binding sites at both the acrosomal and the postacrosomal region (P2: from 6.0% to 32.4%), with decrease (P < 0.001) in the whole head dotted staining (P4: from 45.2% to 4.8%). Likewise, the percentage of cells with WGA-binding sites in the cap (P1) decreased from 59.7% to 41.3% (P = 0.005), while P4 increased to 47.4% from 23.1% (P < 0.001). We detected no differences after 1 h capacitation in the percentage of ConA and PNA-binding site patterns compared with T0.

Along with the differences in the redistribution of the sugar residues described for the T1 condition, changes were recorded after 4 h of capacitation in comparison with T0. The most noticeable was the increase from 26.0% to 45.0% (P = 0.02) of cells with ConA-labeling in the acrosomal as well as the postacrosomal region (P2). We additionally observed an increase in the number of cells that relocated the residues recognized by the AAA lectin to the acrosomal region (P1: from 28.7% to 59.3%; P < 0.001). No significant differences were found in the head spatial distribution of PNA-binding residues in any of the sperm capacitation times under study [Table 1].

Difference in sperm biomarkers

The PCA results provide strong evidence that the sperm biomarkers used can distinguish semen samples [Table 2] and [Figure 3]. Eight principal components were obtained with eigenvalues >1 accounting for 89.3% of the total variance. Nonetheless, little variation appeared after the first two components (≤10% of variance), which is why we considered only the first two principal components (PC1 and PC2), which accounted for 57.1% of the total variance. Similarly, significant differences (Kruskal–Wallis; P < 0.05) were only observed for the first (PC1: χ2 = 27.289; P < 0.001) and second (PC2: χ2 = 7.023; P = 0.008) components when comparing uncapacitated and capacitated samples.
Table 2: Factor loadings corresponding to the first two PCs based on sperm biomarkers within uncapacitated and swim-up capacitated samples

Click here to view
Figure 3: Swim-up capacitation time changes on sperm biomarkers. (a) Binary plot of the first two principal components (PC1 and PC2) accounting for 57.1% of total variance. The ellipses include 95% confidence regions of within-subpopulation sample variability. (b) The labeled rays show the loadings for lectin patterns onto PC1 and PC2 axes (see Table 2 for additional biomarker correlation details). (c) Glycocalyx sketch based on strong lectin correlations in different physiological conditions according to b data. T0 is composed of P4AAA, P1ConA, P1PNA, and P1WGA; T1 is composed of P2AAA, P1ConA, P1PNA, and P4WGA; T4 is composed of P1AAA, P2ConA, P1PNA, and P4WGA. AAA: Aleuria aurantia agglutinin; ConA: Concanavalin A; PNA: Peanut agglutinin; WGA: Wheat germ agglutinin.

Click here to view


PC1 (38.4% of the variance) clearly separated uncapacitated (PC1 negative values) and capacitated samples mainly clustered together [Figure 3]a. Therefore, the sperm biomarkers with the strongest correlations reported significant changes during the capacitation process [Table 2] and [Figure 3]b. PC1 was marked by uniformly high and positive values (r > 0.4; P < 0.01) associated with changes in motility, viability, tyrosine phosphorylation, and spontaneous acrosomal reaction at both capacitation times. Other biomarkers, including sperm concentration and DNA fragmentation, showed significant negative correlations (r > −0.6; P < 0.01) because lower values were recorded during capacitation for both T1 and T4. It became obvious too that several lectin patterns (P4WGA, P1-P2AAA, P1PNA, and P2ConA) were represented mostly on the PC1 axis during capacitation time (r = 0.3–0.7; P < 0.05). Instead, others such as P1WGA, P4AAA, P5AAA, and P5PNA underwent a significant reduction (r = −0.3–−0.8; P < 0.05) [Table 2].

Moreover, sample distribution along PC2 (18.7% of the variance) was mainly affected by interindividual lectin changes after 1 h of capacitation. Interestingly, the significant changes in lectins that occurred during capacitation showed a clearly less variable distribution after 4 h of capacitation [Figure 3]a and [Figure 3]b. In turn, P1 and P5 patterns were conversely distributed, affecting the four different lectins and capacitated sample variability. On the whole, we found that PC2 drew a distinction where P4-5WGA, P2 and P5AAA, P5PNA, and P5ConA patterns received positive loadings (r ≥ 0.4; P < 0.05), as opposed to P1WGA, P1AAA, P1PNA, and P1ConA, which carried negative loadings (r ≥ −0.3; P < 0.05) [Figure 3]b and [Figure 3]c.


  Discussion Top


Although the physiological phenomenon of sperm capacitation was described more than half a century ago,[1] contradictory information still exists about the molecular basis for orchestration of human sperm capacitation. A wide variety of conditions have been employed for capacitation time or medium composition, which, in turn, trigger a great diversity in the elucidation of molecular events.[37] Added to this, the high heterogeneity of human sperm populations, together with the subjectivity of conventional semen analytical techniques, leads to incorrect predictions of sperm functional status.[20],[38] Hence, there is a lack of studies that recognize selection/capacitation time as an influential factor in the fertilization process. Here, we have been the first to undertake a multivariable study of several biomarkers and aspects of sperm structure and function before capacitation and after 1 h and 4 h of swim-up selection under capacitating conditions.

In agreement with previous studies,[39] our findings, based on the use of standard biomarkers, showed that 1 h of capacitation sufficed to recover a sperm subpopulation with high levels of motility and viability. The swim-up recovery of motile sperm directly implies reduction in the total number of recovered spermatozoa (<20%).[26] Our results match this, because the sperm recovery after swim-up was approximately 15% after 1 h and 4 h. Under our experimental conditions, 4 h of capacitation is required to recover a subpopulation with a significant increase of normal sperm forms. In contrast, a previous study recorded a significant increase in normal sperm forms just after 1 h of capacitation.[40] Such a discrepancy is probably related to staining methods.[41] Interestingly, previous observations have highlighted the importance of normal sperm morphology in the binding to the zona pellucida;[42] our results stressed that longer sperm selection times under capacitating conditions enrich the normal forms of morphology.

The ability to undergo the acrosome reaction after induction constitutes a parameter related to sperm quality. Moreover, only the spermatozoa that release their acrosomal content will be able to cross the zona pellucida and fuse with the oocyte.[43] In this context, our results showed that approximately 80% of spermatozoa were incubated to undergo the acrosome reaction by calcium ionophore, regardless of their selection/capacitation time (1 h or 4 h). Therefore, the number of acrosome-reacted spermatozoa depends on the induction time [29] rather than on the sperm selection time in capacitating media. For the spontaneous acrosome reaction, the percentage of reacted spermatozoa clearly increased with the swim-up selection/capacitation time. Likewise, reports have also proved the time-dependent characteristic of the spontaneous acrosome reaction, after 1 h, 3 h, and 5 h of sperm selection/capacitation.[30]

The key sperm capacitation marker is protein phosphorylation on tyrosine residues, because associations exist with hyperactivation, cumulus oophorus penetration, and zona pellucida binding.[11] After 4 h of capacitation, tyrosine phosphorylation was found to increase significantly in different sperm regions. In particular, the principal piece (25.3%) registered a higher percentage of phosphorylation than uncapacitated (3.2%) and 1 h capacitated (17.2%) cells. Likewise, a previous report linked sperm hyaluronic acid binding with time-related increases of tyrosine phosphorylation in the sperm neck and principal piece.[27] Our values suggest that the sperm selection/capacitation process was adequate, because tyrosine phosphorylation increases in a time-related manner.[44]

One of the more popular indicators of sperm function is the detection of fragmented sperm DNA,[23] which impairs function and is a useful predictor of reproductive outcome.[45] The evaluation of DNA damage has also become a complementary test in recent clinical techniques such as magnet-activated cell sorting (MACS), developed additionally to eliminate apoptotic cells.[46] According to one study, DNA fragmentation rates increase significantly with incubation time, from 3.6% (0 h) to 6.2% (4 h), after swim-up sperm selection/capacitation.[47] However, our results showed that extending the selection/capacitation time up to 4 h made it possible to recover a sperm subpopulation with significantly lower DNA damage, from 14.0% in uncapacitated spermatozoa to 2.0% in 4 h capacitated cells. Our findings, thus, provide new insights into the potential of sperm selection time in a capacitating medium with DNA integrity.

The redistribution of glycoconjugates stands out as a major feature of sperm capacitation [10] and plays a prominent role in sperm–zona pellucida binding.[48] Nevertheless, to our knowledge, no information exists on the distribution of lectin-binding patterns during longer combined sperm selection/capacitation times. Our results revealed different sugar redistribution levels depending on the lectin used, as well as a high degree of heterogeneity in the patterns defined (P1–P5). In relation to the sugars recognized by the AAA lectin, a strong relocation in the acrosomal region was observed after 4 h of capacitation. This pattern was shown by approximately 60% of spermatozoa. Similarly, the sugars linked to the ConA lectin underwent a dramatic relocation in both the acrosomal and the postacrosomal region (45%) after a 4 h selection with a capacitating medium. ConA-binding sugar relocation acquires physiological importance, because the presence of those sugars in the postacrosomal region has been linked to proper sperm functionality.[49] For the PNA label pattern, the acrosomal region clearly appears as the principal residue position, regardless of the capacitation time, reaching almost 70% after 4 h. Regarding WGA lectin, the selection/capacitation process also influenced the positioning of its recognized sugar residues. In turn, we found redistribution from the acrosomal region, the most common pattern in the uncapacitated condition, to clusters after just 1 h of capacitation. The lack of differences in WGA pattern percentages at both capacitation times could be related to the WGA-specific sugar recognition, such as sialic acid, and removal during earlier capacitation stages, thus unmasking molecules involved in recognition.[50]

AAA, ConA, PNA, and WGA lectins recognize sugars involved in capacitation and zona pellucida recognition.[48] Therefore, differences in the location of glycocalyx residues during selection/capacitation could explain the lower capacity of spermatozoa to recognize and bind to the zona pellucida when incubated for a relatively short capacitation time (1 h).[16],[51] We additionally identified strong lectin pattern correlations during 4 h capacitation, e.g., P1 in AAA, P2 for ConA, P1 with PNA, and P4 for WGA. The role played by these sugar relocation patterns could be related to proper sperm function and adequate oocyte recognition. Functional tests such as the zona pellucida test would be necessary to confirm these findings.


  Conclusion Top


We found a functional sperm improvement after 4 h swim-up capacitation characterized by an increase in morphologically normal forms; a decrease in DNA fragmentation; a principal piece tyrosine phosphorylation increase; and a wide redistribution of glycocalyx sugars. Even though a shorter sperm preparation time may be more desirable in clinical routines, short-term sperm selection/capacitation may lead to fertilization failures in clinics owing to the lack of time-dependent events that orchestrate capacitation. In short, our data point at 4 h swim-up capacitation as an alternative to obtain a more homogeneous sperm subpopulation with a higher level of structural and functional differentiation. Four hours sperm swim-up capacitation has important potential implications for semen assessment in the future, both for basic research and assisted reproduction techniques in clinical practice.


  Author Contributions Top


PSE, NHR, LRG, and MJGT performed the experiments and collected the data. PSE, AR, IV, and MJGT performed data analyses. PSE, MA, JA, AR, and MJGT conceived the experimental design. All authors contributed to drafting the manuscript. All authors read and approved the final manuscript.


  Competing Interests Top


All authors declared no competing interests.


  Acknowledgments Top


This research was funded by the Human Fertility Chair, the Department of Biotechnology of the University of Alicante (VIGROB-186), and the project of the Spanish Ministry of Economy and Competitiveness (AGL2015-70159-P).



 
  References Top

1.
Austin CR, Bishop MW. Capacitation of mammalian spermatozoa. Nature 1958; 181: 851.  Back to cited text no. 1
    
2.
Chang MC. Fertilizing capacity of spermatozoa deposited into the fallopian tubes. Nature 1951; 168: 697–8.  Back to cited text no. 2
    
3.
Austin CR. Observations on the penetration of the sperm in the mammalian egg. Aust J Sci Res B 1951; 4: 581–96.  Back to cited text no. 3
    
4.
Austin CR. The capacitation of the mammalian sperm. Nature 1952; 170: 326.  Back to cited text no. 4
    
5.
Stival C, Puga Molina LC, Paudel B, Buffone MG, Visconti PE, et al. Sperm capacitation and acrosome reaction in mammalian sperm. Adv Anat Embryol Cell Biol 2016; 220: 93–106.  Back to cited text no. 5
    
6.
Bailey JL. Factors regulating sperm capacitation. Syst Biol Reprod Med 2010; 56: 334–48.  Back to cited text no. 6
    
7.
Chang MC. The meaning of sperm capacitation. A historical perspective. J Androl 1984; 5: 45–50.  Back to cited text no. 7
    
8.
Edwards RG, Bavister BD, Steptoe PC. Early stages of fertilization in vitro of human oocytes matured in vitro. Nature 1969; 221: 632–5.  Back to cited text no. 8
    
9.
Gadella BM, Boerke A. An update on post-ejaculatory remodeling of the sperm surface before mammalian fertilization. Theriogenology 2016; 85: 113–24.  Back to cited text no. 9
    
10.
Liu M. Capacitation-associated glycocomponents of mammalian sperm. Reprod Sci 2016; 23: 572–94.  Back to cited text no. 10
    
11.
Sakkas D, Leppens-Luisier G, Lucas H, Chardonnens D, Campana A, et al. Localization of tyrosine phosphorylated proteins in human sperm and relation to capacitation and zona pellucida binding. Biol Reprod 2003; 68: 1463–9.  Back to cited text no. 11
    
12.
Mortimer ST, Schevaert D, Swan MA, Mortimer D. Quantitative observations of flagellar motility of capacitating human spermatozoa. Hum Reprod 1997; 12: 1006–12.  Back to cited text no. 12
    
13.
Puga Molina LC, Luque GM, Balestrini PA, Marin-Briggiler CI, Romarowski A, et al. Molecular basis of human sperm capacitation. Front Cell Dev Biol 2018; 6: 72.  Back to cited text no. 13
    
14.
Fraser LR. The “switching on” of mammalian spermatozoa: molecular events involved in promotion and regulation of capacitation. Mol Reprod De v 2010; 77: 197–208.  Back to cited text no. 14
    
15.
De Jonge C. Biological basis for human capacitation-revisited. Hum Reprod Update 2017; 23: 289–99.  Back to cited text no. 15
    
16.
Baibakov B, Boggs NA, Yauger B, Baibakov G, Dean J. Human sperm bind to the N-terminal domain of ZP2 in humanized zonae pellucidae in transgenic mice. J Cell Biol 2012; 197: 897–905.  Back to cited text no. 16
    
17.
Buffone MG, Doncel GF, Marin Briggiler CI, Vazquez-Levin MH, Calamera JC. Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation. Hum Reprod 2004; 19: 139–46.  Back to cited text no. 17
    
18.
Buffone MG, Verstraeten SV, Calamera JC, Doncel GF. High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies. J Androl 2009; 30: 552–8.  Back to cited text no. 18
    
19.
Ostermeier GC, Cardona C, Moody MA, Simpson AJ, Mendoza R, et al. Timing of sperm capacitation varies reproducibly among men. Mol Reprod Dev 2018; 85: 387–96.  Back to cited text no. 19
    
20.
Lamb DJ. Semen analysis in 21st century medicine: the need for sperm function testing. Asian J Androl 2010; 1: 64–70.  Back to cited text no. 20
    
21.
Wang C, Swerdloff RS. Limitations of semen analysis as a test of male fertility and anticipated needs from newer tests. Fertil Steril 2014; 6: 1502–7.  Back to cited text no. 21
    
22.
Patel AS, Leong JY, Ramasamy R. Prediction of male infertility by the World Health Organization laboratory manual for assessment of semen analysis: a systematic review. Arab J Urol 2017; 1: 96–102.  Back to cited text no. 22
    
23.
Khatun A, Rahman MS, Pang MG. Clinical assessment of the male fertility. Obstet Gynecol Sci 2018; 61: 179–91.  Back to cited text no. 23
    
24.
Barratt CL, Mansell S, Beaton C, Tardif S, Oxenham SK. Diagnostic tools in male infertility-the question of sperm dysfunction. Asian J Androl 2011; 13: 53–8.  Back to cited text no. 24
    
25.
Oehninger S, Franken DR, Ombelet W. Sperm functional tests. Fertil Steril 2014; 102: 1528–33.  Back to cited text no. 25
    
26.
World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen. Geneva: World Health Organization; 2010.  Back to cited text no. 26
    
27.
Sati L, Cayli S, Delpiano E, Sakkas D, Huszar G. The pattern of tyrosine phosphorylation in human sperm in response to binding to zona pellucida or hyaluronic acid. Reprod Sci 2014; 21: 573–81.  Back to cited text no. 27
    
28.
Cicare J, Caille A, Zumoffen C, Ghersevich S, Bahamondes L, et al. In vitro incubation of human spermatozoa promotes reactive oxygen species generation and DNA fragmentation. Andrologia 2015; 47: 861–6.  Back to cited text no. 28
    
29.
Sosa CM, Pavarotti MA, Zanetti MN, Zoppino FC, De Blas GA, et al. Kinetics of human sperm acrosomal exocytosis. Mol Hum Reprod 2015; 21: 244–54.  Back to cited text no. 29
    
30.
Mansour RT, Serour MG, Abbas AM, Kamal A, Tawab NA, et al. The impact of spermatozoa preincubation time and spontaneous acrosome reaction in intracytoplasmic sperm injection: a controlled randomized study. Fertil Steril 2008; 90: 584–91.  Back to cited text no. 30
    
31.
Cross NL, Overstreet JW. Glycoconjugates of the human sperm surface: distribution and alterations that accompany capacitation in vitro. Gamete Res 1987; 16: 23–35.  Back to cited text no. 31
    
32.
Yamashita K, Kochibe N, Ohkura T, Ueda I, Kobata A. Fractionation of L-fucose-containing oligosaccharides on immobilized Aleuria aurantia lectin. J Biol Chem 1985; 260: 4688–93.  Back to cited text no. 32
    
33.
Brewer F, Bhattacharyya L, Brown RD 3rd, Koenig SH. Interactions of concanavalin A with a trimannosyl oligosaccharide fragment of complex and high mannose type glycopeptides. Biochem Biophys Res Commun 1985; 127: 1066–71.  Back to cited text no. 33
    
34.
Pereira ME, Kabat EA, Lotan R, Sharon N. Immunochemical studies on the specificity of the peanut (Arachis hypogaea) agglutinin. Carbohydr Res 1976; 51: 107–18.  Back to cited text no. 34
    
35.
Monsigny M, Roche AC, Sene C, Maget-Dana R, Delmotte F. Sugar-lectin interactions: how does wheat-germ agglutinin bind sialoglycoconjugates? Eur J Biochem 1980; 104: 147–53.  Back to cited text no. 35
    
36.
Gómez-Torres MJ, Avilés M, Girela JL, Murcia V, Fernandez-Colom PJ, et al. Characterization of the lectin binding pattern in human spermatozoa after swim-up selection. Histol Histopathol 2012; 27: 1621–8.  Back to cited text no. 36
    
37.
De Jonge C. Biological basis for human capacitation. Hum Reprod Update 2005; 11: 205–14.  Back to cited text no. 37
    
38.
Keel BA. How reliable are results from the semen analysis? Fertil Steril 2004; 82: 41–4.  Back to cited text no. 38
    
39.
Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol 2003; 1: 108.  Back to cited text no. 39
    
40.
Oehninger S, Acosta R, Morshedi M, Philput C, Swanson RJ, et al. Relationship between morphology and motion characteristics of human spermatozoa in semen and in the swim-up sperm fractions. J Androl 1990; 11: 446–52.  Back to cited text no. 40
    
41.
Henkel R, Schreiber G, Sturmhoefel A, Hipler UC, Zermann DH, et al. Comparison of three staining methods for the morphological evaluation of human spermatozoa. Fertil Steril 2008; 89: 449–55.  Back to cited text no. 41
    
42.
Liu DY, Garrett C, Baker HW. Low proportions of sperm can bind to the zona pellucida of human oocytes. Hum Reprod 2003; 18: 2382–9.  Back to cited text no. 42
    
43.
Mahi CA, Yanagimachi R. Capacitation, acrosome reaction, and egg penetration by canine spermatozoa in a simple defined medium. Gamete Res 1978; 1: 101–9.  Back to cited text no. 43
    
44.
Liu DY, Clarke GN, Baker HW. Tyrosine phosphorylation on capacitated human sperm tail detected by immunofluorescence correlates strongly with sperm-zona pellucida (ZP) binding but not with the ZP-induced acrosome reaction. Hum Reprod 2006; 21: 1002–8.  Back to cited text no. 44
    
45.
Muratori M, Marchiani S, Maggi M, Forti G, Baldi E. Origin and biological significance of DNA fragmentation in human spermatozoa. Front Biosci 2006; 11: 1491–9.  Back to cited text no. 45
    
46.
Said T, Agarwal A, Grunewald S, Rasch M, Baumann T, et al. Selection of nonapoptotic spermatozoa as a new tool for enhancing assisted reproduction outcomes: an in vitro model. Biol Reprod 2006; 74: 530–7.  Back to cited text no. 46
    
47.
Zhang XD, Chen MY, Gao Y, Han W, Liu DY, et al. The effects of different sperm preparation methods and incubation time on the sperm DNA fragmentation. Hum Fertil (Camb) 2011; 14: 187–91.  Back to cited text no. 47
    
48.
Tecle E, Gagneux P. Sugar-coated sperm: unraveling the functions of the mammalian sperm glycocalyx. Mol Reprod Dev 2015; 82: 635–50.  Back to cited text no. 48
    
49.
Bains HK, Sehgal S, Bawa SR. Human sperm surface mapping with lectins. Acta Anat (Basel) 1992; 145: 207–11.  Back to cited text no. 49
    
50.
Lassalle B, Testart J. Human zona pellucida recognition associated with removal of sialic acid from human sperm surface. J Reprod Fertil 1994; 101: 703–11.  Back to cited text no. 50
    
51.
Jansen CH, Elisen MG, Leenstra CW, Kaaijk EM, van Stralen KJ, et al. Longer time interval between semen processing and intrauterine insemination does not affect pregnancy outcome. Fertil Steril 2017; 108: 764–9.  Back to cited text no. 51
    


    Figures

  [Figure 1], [Figure 2], [Figure 3]
 
 
    Tables

  [Table 1], [Table 2]



 

 
Top
 
 
  Search
 
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)  

 
  In this article
Abstract
Introduction
Materials and Me...
Results
Discussion
Conclusion
Author Contributions
Competing Interests
Acknowledgments
References
Article Figures
Article Tables

 Article Access Statistics
    Viewed3951    
    PDF Downloaded554    

Recommend this journal