|Ahead of print publication
MicroRNA expression profile in chronic nonbacterial prostatitis revealed by next-generation small RNA sequencing
Li Zhang1,2,3, Yi Liu1,2,3, Xian-Guo Chen1,2,3, Yong Zhang1, Jing Chen1, Zong-Yao Hao1,2,3, Song Fan1,2,3, Li-Gang Zhang1, He-Xi Du1, Chao-Zhao Liang1,2,3
1 Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China
2 Institute of Urology, Anhui Medical University, Hefei 230022, China
3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230022, China
|Date of Submission||25-Apr-2018|
|Date of Acceptance||28-Sep-2018|
|Date of Web Publication||25-Dec-2018|
Department of Urology, The First Affiliated Hospital of Anhui Medical University; Institute of Urology; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230022
Source of Support: None, Conflict of Interest: None
MicroRNAs (miRNAs) are considered to be involved in the pathogenic initiation and progression of chronic nonbacterial prostatitis (CNP); however, the comprehensive expression profile of dysregulated miRNAs, relevant signaling pathways, and core machineries in CNP have not been fully elucidated. In the current research, CNP rat models were established through the intraprostatic injection of carrageenan into the prostate. Then, next-generation sequencing was performed to explore the miRNA expression profile in CNP. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) bioinformatical analyses were conducted to reveal the enriched biological processes, molecular functions, and cellular components and signaling pathways. As a result, 1224, 1039, and 1029 known miRNAs were annotated in prostate tissues from the blank control (BC), normal saline injection (NS), and carrageenan injection (CAR) groups (n = 3 for each group), respectively. Among them, 84 miRNAs (CAR vs BC) and 70 miRNAs (CAR vs NS) with significantly different expression levels were identified. Compared with previously reported miRNAs with altered expression in various inflammatory diseases, the majority of deregulated miRNAs in CNP, such as miR-146b-5p, miR-155-5p, miR-150-5p, and miR-139-5p, showed similar expression patterns. Moreover, bioinformatics analyses have enriched mitogen-activated protein kinase (MAPK), cyclic adenosine monophosphate (cAMP), endocytosis, mammalian target of rapamycin (mTOR), and forkhead box O (FoxO) signaling pathways. These pathways were all involved in immune response, which indicates the critical regulatory role of the immune system in CNP initiation and progression. Our investigation has presented a global view of the differentially expressed miRNAs and potential regulatory networks containing their target genes, which may be helpful for identifying the novel mechanisms of miRNAs in immune regulation and effective target-specific theragnosis for CNP.
Keywords: chronic nonbacterial prostatitis; immune; inflammation; miRNA; next-generation sequencing
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|How to cite this URL:|
Zhang L, Liu Y, Chen XG, Zhang Y, Chen J, Hao ZY, Fan S, Zhang LG, Du HX, Liang CZ. MicroRNA expression profile in chronic nonbacterial prostatitis revealed by next-generation small RNA sequencing. Asian J Androl [Epub ahead of print] [cited 2019 Mar 20]. Available from: http://www.ajandrology.com/preprintarticle.asp?id=248810
| Introduction|| |
Inflammation is regarded as the driving factor in many diseases, including atherosclerosis, cancer, autoimmunity, and infections. It is broadly defined as a protective response by the organism in the presence of invaded pathogens or endogenous stress signals that subsequently results in the elimination of initial injury causes, necrotic cell clearance, and eventually facilitates tissue repair. Immunological processes are often involved in inflammatory responses. The pro-inflammatory cytokines and chemokines released by host cells such as tumor necrosis factor (TNF) and interleukin-1β (IL-1β) have autocrine and paracrine effects, leading to the local activation of macrophages and neutrophils. Meanwhile, cytokine-mediated activation of endothelial cells can increase vascular permeability and elicit the entrance of immune cells into tissues. Next, infiltrated type 1 helper T (TH1) cells secrete interferon-γ (IFN-γ) to activate neutrophils. Meanwhile, IL-17-producing helper T cells (TH17 cells) and innate lymphoid cells could secrete IL-22 to further stimulate the production and release of antimicrobial peptides. For instance, autoimmune diseases such as rheumatoid arthritis and inflammatory skin diseases caused by immunological imbalances have been frequently linked with chronic lesion inflammation. Likewise, pro-inflammatory cytokines can promote the progression of immune diseases by generating pathogenic B- and T-cells. Therefore, immune responses are important parts of inflammation.
Chronic nonbacterial prostatitis (CNP) is a commonly observed distress in male patients that is characterized by genital or pelvic discomfort, voiding and pain symptoms, and even by sexual dysfunction; it is much more common than chronic bacterial prostatitis (CBP), comprising more than 90% of chronic prostatitis. To date, the etiology of CNP remains largely unknown. During the past decades, several hypotheses have been proposed to explain CNP pathogenesis including defective urothelial integrity and function, cryptic infections, autoimmunity, endocrine imbalances, neuroplasticity, and psychosocial conditions. Recently, accumulating evidences from studies in patients and animal models have raised the possibility that CNP is a consequence of dysregulated inflammation associated with autoimmune disorders. Immunization with prostate gland homogenates or purified prostate proteins such as prostate–steroid binding protein and prostate acid phosphatase or peptides could induce prostatic inflammation and chronic pelvic pain. Consistently, T-cells with self-reactivity to prostate-specific antigen (PSA), prostatic acid phosphatase, and other prostatic and seminal plasma proteins have been successfully isolated from CNP patients.
MicroRNAs (miRNAs) are posttranscriptional repressors of gene expression that play critical roles in a variety of physiological and pathological processes including development, differentiation, aging, and cancer. Notably, miRNAs are also widely accepted to participate in inflammation and lymphocyte homeostasis and allergic responses through coordinated modulation of multiple target genes. For example, miRNAs can execute the functional regulation of immune cells such as Th cells or inflammatory signal pathways such as the nuclear factor kappa-light-chain enhancer of activated B-cell (NF-κB) network in inflammatory or autoimmune diseases, indicating the crucial role of miRNAs in both inflammation and immune responses.
Recently, few studies have focused on the miRNA in CNP. One study revealed a decreased risk of erectile dysfunction (ED) in patients with CNP who were carriers of the miR-146a rs2910164 C allele, while another study found that N-acetylcysteine could ameliorate carrageenan-induced prostatitis and prostatic pain through miR-141-regulated Kelch-like ECH-associated protein-1/nuclear factor erythroid-2-related factor 2 (Keap1/Nrf2) signaling pathways. In 2018, a study evaluated the miRNA expression profile in expressed prostatic secretion (EPS). However, EPS is limited when illustrating the integral immune response and inflammation status of prostate tissue. Therefore, we comprehensively evaluated the dysregulated expression profile of miRNAs in CNP prostate samples using next-generation sequencing (NGS) technology, aiming to fully understand the role of miRNAs in CNP initiation and progression. Furthermore, bioinformatics analyses were applied to enrich immune-related biological processes, molecular functions, cellular components, and signaling pathways, the efforts of which would help explore the immune regulatory mechanisms in CNP.
| Materials and Methods|| |
Eighteen healthy adult male Wistar rats (aged 8 weeks, 250 ± 20 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd., Beijing, China. The animals were housed in groups of three in plastic cages with soft bedding and free access to food and water under a 12/12 h reversed light-dark cycle. All animals were acclimated for 1 week before experimental procedures. All experimental protocols were approved by the Animal Research Ethical Committee of Anhui Medical University, Hefei, China. All surgeries were performed under chloral hydrate anesthesia, and all efforts were made to minimize suffering.
Carrageenan-induced CNP model establishment
Nine Wistar rats were randomly divided into the following three groups: (1) blank control (without any interference, BC) group, (2) normal saline injection (NS) group, and (3) carrageenan injection (CAR) group. For injection of carrageenan, rats were anesthetized with an intraperitoneal injection of 4% chloral hydrate (A600288; Sangon Biotech, Shanghai, China) and fixed in a supine position. Then, the lower abdomen above the rats' penis was shaved and the skin in this area was disinfected using three applications of 10% povidone-iodine solution (A606166; Sangon Biotech). A small midline incision was made in the disinfected area, and the bladder and prostate were carefully exposed. Using a microsyringe, 50 μl of sterile suspension of 5% carrageenan suspension (22049; Sigma-Aldrich, St. Louis, MO, USA) was injected into each ventral lobe in the prostate gland. A commensurate amount of sterile normal saline was injected in the NS group and nothing was injected in the BC group. After checking for bleeding at the dissection site, the wound was closed in layers.
One week later, all the rats were deprived of food and water for 12 h and then sacrificed under anesthesia with chloral hydrate. Prostate samples were collected and rinsed with phosphate-buffered saline (PBS) to remove adherent blood and fat tissues. One ventral lobe was formalin fixed for hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining and the other ventral lobe was cut into pieces, stored in RNAlater solution (AM7020; Ambion, Carlsbad, CA, USA), and frozen at −80°C according to a standard operation procedure.
The rat prostate tissues were fixed in 10% formalin, dehydrated, and then embedded in paraffin. Sections about 4-μm thickness were sliced from each embedded tissue and stained with HE. For HE staining, the standard protocol was followed. Briefly, 4 μm-sized paraffin-embedded tissue slides were dried at 65°C for 2 h and de-paraffinized with xylene (Sinopharm Chemical Reagent, Shanghai, China) for four consecutive times (10 min each time). The sections were then dipped into 100%, 95%, 80%, and 70% ethanol for 5 min each time. The sections were rinsed with tap water for 2 min to remove ethanol and then stained with hematoxylin for 5 min, followed by tap water washing for 3 min. Subsequently, the slides were dipped into 0.1% HCl (Sinopharm Chemical Reagent) for three times and washed with tap water, followed by 50°C water incubation for 5 min. Later on, the slides were stained with eosin for 2 min and then washed with tap water for 2 min. Finally, after dehydration and dipping the slides into xylene, 2–3 drops of mountant were added onto the slide for observation under a microscope (IX 71; Olympus, Tokyo, Japan).
For cyclooxygenase-2 (COX-2) expression evaluation, IHC analysis was performed with COX-2 antibody (E303; Spring Bioscience, Pleasanton, CA, USA) on whole-tissue sections. Briefly, after routine deparaffinization and rehydration, the slides were treated with 10 mmol l−1 sodium citrate buffer (pH = 6.0; ZLI-9065; Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China) and boiled for 10 min to retrieve antigen. The endogenous peroxidase activity was quenched by 3% hydrogen peroxide (Sinopharm Chemical Reagent) for 15 min. After PBS (E607008; Sangon Biotech) washing for 15 min, the slides were blocked by bovine serum albumin (BSA, 37520; Thermo Fisher Scientific, Waltham, MA, USA) in a wet box and incubated for 10 min. BSA was then removed and the slides were incubated with primary COX-2 antibody with 1:100 dilutions overnight at 4°C. The next day, the incubated slides were washed with PBS for three times (5 min each time) and further incubated with poly-horseradish peroxidase (HRP) goat IgG (SP-9000; Zhongshan Golden Bridge Biotechnology Co., Ltd) for 15 min. After 15-min PBS washing (5 min each time), streptavidin/HRP was added to incubate for 15 min, and then washed with PBS for 15 min (5 min each time). Subsequently, detection was made using 3, 3′-diaminobenzidene as instructed (DAB kit, ZLI-9018; Zhongshan Golden Bridge Biotechnology Co., Ltd). Finally, the slides were counterstained with hematoxylin before microscopic observation. Cytoplasmic staining was scored as negative (-), weak (+), moderate (++), or strong (+++). A positive result required both cytoplasmic staining in >10% of cells and moderate-to-strong intensity, as previously described. Quantitative analysis of the brown positive staining was performed using Image-Pro Plus 6.0 (Media Cybernetics, Rockville, MD, USA).
Enzyme-linked immunosorbent assay (ELISA)
Serum IFN-γ concentrations were determined by using rat ELISA kit assay (E-EL-R0009C; Elabscience, Wuhan, China) according to the manufacturer's protocol. Briefly, 48-well plates were incubated with 100 μl standard solution or serum samples obtained from rats (three rats for each group) at 37°C for 90 min and the liquid was removed. After 100-μl biotinylated detection antibody incubation for 1 h at 37°C, each well was washed three times. Subsequently, 100-μl HRP conjugate was added and incubated at 37°C for 30 min, and then washed five times. Then, 90-μl substrate reagent was added and reacted at 37°C for 15 min. Finally, 50-μl stop solution was added. Absorbances were measured at 450 nm immediately. The sensitivity limit of the method was 18.75 pg ml−1.
RNA quality check, amplification, and NGS
Total RNA was collected from the three groups of tissues using the TRIzol reagent (15596-026; Life Technologies, Carlsbad, CA, USA). RNA quality check was assessed using an A260/A280 ratio (A: absorbance) between 1.8 and 2.2, an OD260/230 radio (OD: optical density) more than 2.0, a sample concentration >200 ng μl−1, and an RIN over 7 using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then, ligation of 3'- and 5'-adapters was completed for each qualified RNA with T4 RNA Ligases (Thermo Fisher Scientific) with the presence of a phosphate group on the 5'-end and a hydroxyl group on the 3'-end of mature miRNAs. Next, reverse transcription (RT) was performed to make cDNA with the SuperScript IV revertase (Thermo Fisher Scientific), followed by PCR amplification. The acquired miRNA library was separated and purified by high-resolution polypropylene acyl ammonia gel electrophoresis (PAGE), and finally the miRNA library quality check was completed with a Qubit and an Agilent 2100 Bioanalyzer before NGS (Hiseq 2500; Illumina, San Diego, CA, USA).
NGS data analyses and novel miRNA identification
The miRNA sequencing data were further analyzed according to the methods described in a previous publication. Briefly, clean reads were acquired via the following steps: removing adaptor sequences, deleting sequences without 3'-adapters and inserting sequences, and omitting sequences with a Q20 ratio <60% and a length >18–36 bp using the FASTX-Toolkit software (http://hannonlab.cshl.edu/fastx_toolkit/). The qualified sequences were analyzed based on their length peak and underwent homogeneous BLAST alignment (http://blast.ncbi.nlm.nih.gov/) to mature miRNAs in miRBase (http://www.mirbase.org/), as well as reference genome alignment using Rfam (http://rfam.xfam.org/). Identification of known miRNAs was conducted by using the miRDeep2 tool (https://www.mdc-berlin.de/8551903/en/), which was also routinely used for novel miRNA exploration.
Bioinformatics analyses for differentially expressed miRNAs
The approaches described for the enrichment of functions and signaling pathways were frequently used in the present study. Briefly, target genes of differentially expressed miRNAs from three groups were predicted using RNAhybrid (http://bibiserv.techfak.uni-bielefeld.de/rnahybrid/) and miRanda (http://www.microrna.org/microrna/home.do) and analyzed with R package clusterProfiler (http://bioconductor.org/packages/release/bioc/html/clusterProfiler.html) before undergoing Gene Ontology (GO; http://www.geneontology.org) and Kyoto Encyclopedia of Genes and Genomes (KEGG; http://www.genome.jp/kegg/) enrichment analyses. After mapping the potential target genes to the dataset of GO annotations and KEGG pathway datasets, the enriched biological processes, molecular functions, cellular components, and signaling pathways were eventually sorted.
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) verification for altered miRNA and gene expression
cDNA synthesis was performed using a FastQuant RT kit (with gDNAase) following the manufacturer's instructions (KR10; Tiangen Biotech, Beijing, China). The abundance of individual miRNA was detected via an Applied Biosystems 7500 PCR System (Applied Biosystems, Foster City, CA, USA) using SuperReal PreMix Plus (SYBR Green; FP215, Tiangen Biotech). The specific RT and qPCR primers for all miRNAs and genes were purchased from Sangon Biotech and listed in [Supplementary Table 1] [Additional file 1]. The qPCR procedures were performed according to the protocols provided by the manufacturers. Briefly, triplicate reactions were performed at 95°C for 15 min, and the subsequent 40 amplification cycles were conducted at 95°C for 10 s and 60°C for 32 s. Meanwhile, U6 snRNA or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as an internal normalized control. Relative miRNA abundances were calculated using 2−ΔΔCt (threshold cycle) formula, where Δ Ct = CtmiRNA/gene− CtU6 snRNA/GAPDH and ΔΔ Ct =Δ CtCAR−Δ CtBC/NS. The miRNA/gene abundance differences between CAR and BC or NS group were analyzed by using unpaired t-tests. P < 0.05 indicated a statistically significant difference.
The differences among groups were analyzed using a randomized block design analysis of variance with SPSS version 10.0 software (SPSS Inc., Chicago, IL, USA). P < 0.05 was considered statistically significant.
| Results|| |
Inflammatory cell accumulation and COX-2 expression in CNP tissues
Intraprostatic carrageenan injection induced prostatic edema and increased inflammatory cell accumulation. The majority of inflammatory cells were neutrophils and presented to the interstitial space in the CAR group, while no inflammatory cell infiltration was observed in the BC or NS group [Supplementary Figure 1] [Additional file 9]. Consistently, COX-2 protein levels were significantly higher (P < 0.01) in the prostates from the CAR group compared with those of the BC and NS groups [Supplementary Figure 2] [Additional file 10]. The HE and IHC staining results indicated that the CNP models were successfully established.
Serum IFN-γ levels were elevated in CNP rats
IFN-γ has been widely recognized as a signature pro-inflammatory cytokine that plays a central role in inflammation, and several studies have suggested a crucial role for IFN-γ in the pathogenesis of experimental prostatitis in animal models., Thus, we have evaluated the serum concentration of the pro-inflammatory cytokine in our carrageenan-induced prostatitis model. As a result, concentrations of IFN-γ in BC and NS groups were 24.41 ± 1.50 pg ml−1 and 19.55 ± 1.13 pg ml−1, respectively, while CAR group showed a significantly increased level of IFN-γ at 44.11 ± 1.22 pg ml−1 (mean ± standard error of the mean [s.e.m.]; CAR vs BC, P = 0.0005; CAR vs NS, P = 0.0001). The elevated serum levels verified that CAR injection indeed elicited CNP in rats [Supplementary Figure 3] [Additional file 11].
Overview of miRNA-NGS data from CNP prostate specimens
The majority of the clean sequencing reads were 22 nucleotides (nt) in size, followed by 21-nt or 23-nt RNA fragments, which were within the typical size range of registered miRNAs [Figure 1]a, [Figure 1]b, [Figure 1]c. The 18–36-nt qualified sequences were further analyzed based on their length peak. The counts of mature miRNAs aligned to miRBase for each sample were as follows: 626, 602, and 564 for the BC group; 243, 632, and 608 for the NS group; and 425, 453, and 525 for the CAR group. After comparing the sequences from each sample to the Rfam database, the miRNA sequence number and proportion of the genome for miRNAs are presented in [Figure 1]d, [Figure 1]e, [Figure 1]f, revealing that the ratio of miRNAs in each sample was >85%.
|Figure 1: Length distribution of clean reads from small RNA NGS and Rfam alignment analyses in different sequencing samples. Length distribution (nucleotides) of clean reads from small RNA NGS of prostatic tissues (a) without any treatment (BC), (b) treated with NS, and (c) treated with CAR. The ratio of different smRNAs (miRNAs, rRNAs, scRNAs, snoRNAs, snRNAs, and tRNAs) compared with Rfam database from small RNA NGS of prostatic tissues (d) without any treatment (BC), (e) treated with NS, and (f) treated with CAR. NGS: next-generation sequencing; BC: blank control group; NS: normal saline group; CAR: carrageenan group.|
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Characteristics of top abundant known and novel miRNAs
To analyze the most abundant miRNAs in the prostate tissues, we listed the top twenty highly expressed miRNAs with relatively more read count in decreasing order from the known miRNAs in the CAR, NS, and BC groups, respectively, whose expression profiles were indicated by the average read counts. In the inflammatory prostate tissues, rno-miR-10a-5p, rno-miR-148a-3p, rno-miR-21-5p, rno-miR-10b-5p, rno-miR-143-3p, rno-miR-99a-5p, rno-miR-99b-5p, rno-miR-200b-3p, rno-miR-100-5p, and rno-miR-183-5p were the top ten abundant miRNAs. The highly expressed miRNAs in the NS group were rno-miR-148a-3p, rno-miR-10a-5p, rno-miR-200b-3p, rno-miR-143-3p, rno-miR-99a-5p, rno-miR-10b-5p, rno-miR-21-5p, rno-miR-100-5p, rno-miR-183-5p, and rno-miR-99b-5p. Correspondingly, the miRNAs possessing the highest expression levels in the BC group were rno-miR-148a-3p, rno-miR-200b-3p, rno-miR-10a-5p, rno-miR-143-3p, rno-miR-99a-5p, rno-miR-21-5p, rno-miR-10b-5p, rno-miR-152-3p, rno-miR-100-5p, and rno-miR-200c-3p. From these data, we found that the most abundantly expressed miRNAs in the prostate tissues from the three groups were approximately the same [Supplementary Table 2][Additional file 2], indicating the identical histological origin of the paired samples undergoing NGS. Furthermore, 115 novel miRNAs were identified and presented in [Supplementary Table 3] [Additional file 3], whose roles in the development and progression of prostatitis require further investigation.
Differentially expressed profile of miRNAs in CNP tissues
When comparing the distinct levels of miRNA abundance among the three groups, two quantities were of great importance: one is the P value and another is the log2(fold change) value. The P value responses to whether the difference in expression levels was statistically significant, while the log2(fold change) value could illustrate the magnitude of the miRNA abundance differences. A log2(fold change) value above 0 indicates upregulation and a log2(fold change) value below 0 indicates decreased expression. Notably, even very large log2(fold change) values may have insignificant P values caused by considerable variabilities. Similarly, relatively small magnitude in the differences may also possess tiny P values due to the highly consistent technical replicates within each sample. Thus, a useful visualization method called a “volcano plot,” which can simultaneously exhibit log2(fold change) and P values on the X- and Y-axes, respectively, has been introduced to conduct our analysis. Generally, points located in the upper-left or upper-right regions of the plot and indicated with green or red color possess both small P values (<0.05) and high log2(fold change). In this comparative study, the volcano plot generated by the miRNA profiles from the three groups is shown in [Figure 2]a and [Figure 2]b. As a result, 84 (CAR vs BC) and 70 (CAR vs NS) significantly dysregulated known miRNAs were found after adjusting for multiple testing. Among them, the top twenty upregulated and downregulated miRNAs are shown in [Table 1] (CAR vs BC) and [Table 2] (CAR vs NS), respectively. Meanwhile, hierarchical clustering analysis was performed to cluster the same or similar differentially expressed miRNAs [Figure 2]c and [Figure 2]d.
|Figure 2: The overview of the volcano plot generated by miRNA profile and clustering heat maps of differentially expressed miRNA. The volcano plot generated by miRNA expression profile from small RNA NGS between prostatic tissues (a) treated with CAR or without any treatment (BC) and (b) treated with CAR or NS. The red points in the plot represent significantly upregulated miRNAs and the green points represent significantly downregulated miRNAs, while the blue points show no statistically significant differences due to either small fold change (<2) or insignificant P values. The heat map of miRNA expression profile between prostatic tissues (c) treated with CAR or without any treatment (BC) and (d) treated with CAR or NS. For c and d, each column represents a certain sample and lines represent different genes; the corresponding color represents the amount of gene expression in the sample: red color indicates higher expression and green represents lower expression. DEG: differentially expressed genes; BC: blank control group; NS: normal saline group; CAR: carrageenan group.|
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|Table 1: A collection of top twenty upregulated and downregulated miRNAs detected by next-generation sequencing in prostate tissues (carrageenan group vs blank control group)|
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|Table 2: A collection of top twenty upregulated and downregulated miRNAs detected by next.generation sequencing in prostate tissues (carrageenan group vs normal saline group)|
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As expected, the twenty upregulated and downregulated miRNAs between the CAR versus BC groups and the CAR versus NS groups mostly overlapped [Table 1] and [Table 2], verifying the significant regulatory roles of miRNAs in the progression of inflammation in CNP. Meanwhile, the normalized expression levels of aforementioned top differentially expressed miRNAs in BC, NS, and CAR groups are shown in [Supplementary Table 4] [Additional file 4]. The most abundantly upregulated miRNAs in the prostate tissues were rno-miR-155-5p, rno-miR-501-3p, rno-miR-1956-3p, rno-miR-212-5p, and rno-miR-146a-3p for CAR versus BC and rno-miR-155-5p, rno-miR-184, rno-miR-132-3p, rno-miR-501-3p, and rno-miR-1956-3p for CAR versus NS. Meanwhile, rno-miR-33-5p, rno-miR-136-5p, rno-miR-19a-3p, rno-miR-150-5p, and rno-miR-130a-3p were the top five most abundantly downregulated miRNAs in the prostate tissues for CAR versus BC, while rno-miR-33-5p, rno-miR-136-5p, rno-miR-130a-3p, rno-miR-19a-3p, and rno-let-7g-3p were the five most decreased miRNAs for CAR compared with NS. Besides, we have compared the differential expression between BC and NS groups, the results showed almost no significant difference of the miRNA expression levels between the two groups except for rno-miR-124-3p and rno-miR-150-5p, indicating that the altered expression between CAR versus BC and CAR versus NS was reliable [Supplementary Table 5] [Additional file 5] and [Supplementary Table 6] [Additional file 6].
Validation of differentially expressed miRNAs revealed by NGS
To better evaluate the accuracy of NGS data, we have established a new batch of CNP model rats and corresponding control groups. Validation of altered miRNA expression in CNP tissues profiled by NGS was performed by qRT-PCR in BC, NS, and CAR groups. We randomly picked five upregulated and five downregulated miRNAs among the differentially expressed miRNAs to conduct the analysis, and each expression abundance was presented using the normalized fold change of CAR group versus BC and NS groups. As a result, the altered expression tendency of all the ten deregulated miRNAs was consistent with the data obtained from NGS technology. Besides, overwhelming majority of the tested miRNAs showed significant differences between CAR versus BC and CAR versus NS. Among all the compared miRNAs, significant differences were only not found in miR-130a-3p and miR-26a-3p between CAR and NS, which together indicated that the expression levels of miRNAs detected by NGS were reliable [Figure 3].
|Figure 3: Relative expression of (a) five upregulated and (b) five downregulated miRNAs in BC, NS, and CAR groups at day 7 after model establishment (n =3 for each group). Data are presented as mean ± s.e.m.*P < 0.05,**P < 0.01 BC: blank control group; NS: normal saline group; CAR: carrageenan group; s.e.m: standard error of the mean.|
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GO enrichment analysis of differentially expressed genes in the CNP prostate tissues
The essential biological functions of the putative target genes were classified via the GO analysis. To better understand the biological behaviors of the known deregulated miRNAs, potential target genes and biological functions targeted by these miRNAs were predicted. The top ten most enriched GO terms for biological processes (BPs), cellular components (CCs), and molecular functions (MFs) are presented in [Supplementary Table 7] [Additional file 7], [Supplementary Table 8] [Additional file 8] and [Supplementary Figure 4] [Additional file 12]. Of these genes, the putative target genes of deregulated miRNAs appeared to be mostly enriched for CC such as cell, cell part, intracellular, intracellular part, and organelle, when comparing the CAR group with either the BC or NS group. Meanwhile, the most enriched MFs were binding, protein binding, ion binding, organic cyclic compound binding, and heterocyclic compound binding, and the most enriched BPs were cellular process, single-organism process, metabolic process, organic substance metabolic process, and cellular metabolic process for the CAR versus BC or CAR versus NS comparison. Correspondingly, the putative target genes of deregulated miRNAs between CAR and NS appeared to mostly overlap with the CAR versus BC comparison.
KEGG pathway analysis of differentially expressed genes in the CNP prostate tissues
After GO analysis, the putative target genes of the differentially expressed miRNAs in the prostate specimens were examined by KEGG for pathway enrichment analysis. The top ten enriched KEGG pathways are presented in [Figure 4]. Based on the proportion of differentially expressed genes in all the annotated genes, Q-values (adjusted P values), and number of genes enriched in specific pathways, we have annotated the most enriched pathways. When comparing CAR with BC, these pathways were tightly linked to cancer, endocytosis, proteoglycans in cancer, axon guidance, and forkhead box O (FoxO). When comparing CAR with NS, these pathways were tightly linked to cancer, mitogen-activated protein kinase (MAPK), proteoglycans in cancer, cyclic adenosine monophosphate (cAMP), and calcium signaling pathways. Furthermore, to confirm that the aforementioned deregulated miRNAs could contribute to affecting the enriched KEGG pathways, we have selected two essential genes cAMP and mammalian target of rapamycin (mTOR) for mRNA expression validation from “cAMP signaling pathway” and “mTOR signaling pathway.” As a result, cAMP showed significant increased expression in CAR group, while mTOR presented significant decreased expression in CAR group, compared with BC and NS groups (P < 0.01; [Supplementary Figure 5] [Additional file 13]). These results indicated that miRNA expression changes in fact caused altered gene expression in enriched pathway, which may further initiate the activation or depression of the following signaling transduction involved in inflammation and immune response.
|Figure 4: KEGG enrichment dot plot for target genes of differentially expressed miRNAs (CAR vs BC and CAR vs NS). The top ten enriched KEGG pathways are presented. The X-axis represents enrichment factor, indicating the proportion of differentially expressed genes in all annotated genes on certain signaling pathway. The Y-axis represents log10(Q-values). Enriched pathways with higher X and Y values possessed better statistical significances. (a) CAR versus BC; (b) CAR versus NS. BC: blank control group; NS: normal saline group; CAR: carrageenan group; KEGG: Kyoto Encyclopedia of Genes and Genomes; FoxO: forkhead box O; AGE-RAGE: advanced glycation end products-receptor for advanced glycation end products; MAPK: mitogen-activated protein kinase; cAMP: cyclic adenosine monophosphate; mTOR: mammalian target of rapamycin; Wnt: wingless/int 1; Q-values: adjusted P values.|
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| Discussion|| |
CNP is a complex and frustrating syndrome caused by unknown pathological mechanisms with a lack of effective therapeutics. Massive efforts have been made to uncover the pivotal factors that promote the occurrence and development of CNP. Although only one-third have inflammation on biopsy, and how chronic pelvic pain develops and whether it could be mechanistically linked with inflammation were still controversial, the crucial role of inflammation in CNP and symptoms such as chronic pelvic pain should not be unnoticed, as unresolved chronic inflammation may potentiate tissue injury resulting in central sensitization and eventually cause chronic pelvic pain.,, Recently, researches in biomedical science studies have indicated that inflammation and immune responses coexist in various kinds of diseases. Correspondingly, immune mechanisms have been reported to be involved in CNP etiology, pathogenesis, and symptom development. However, full understanding of the exact molecular mechanisms, especially the immune regulatory networks involved in CNP, remains largely absent. Notably, an increasing number of studies have shown that microRNAs, a class of evolutionally conserved and endogenously expressed RNAs, could be considered as critical regulators of gene expression at posttranscriptional levels. MiRNAs are involved in the pathogenesis of a broad spectrum of human disorders, including metabolic and inflammatory diseases. Accordingly, as CNP has shown complex symptom features such as prostate inflammation and immune disorders, it is of great importance to generate the differentially expressed miRNA profiles in prostatitis tissues to further understand the miRNA regulatory networks in CNP initiation and progression, especially to clearly understand the possible role of the immune system in CNP.
Recently, a newly developed NGS technology has been widely used for miRNA quantitative studies, due to the higher test specificity and sensitivity to detect extremely low-abundance miRNAs that may not be detected by the traditional quantitative assays. Based on NGS, bioinformatics analyses of deregulated miRNA expression signatures have further enriched several significant GO terms and signaling pathways, which are considered to be related to inflammatory diseases such as osteoarthritis and rheumatoid arthritis (RA). However, no systematic studies have been conducted on miRNAs profiling in CNP tissues by NGS.
In the present study, we used NGS technology to identify miRNAs prepared from prostate tissues from a CNP animal model. We quantified a total of 20 048 744, 15 070 023, and 9 183 863 sequencing read counts with typical 18–36 nts length for the BC, NS, and CAR groups, respectively, following alignment to the rat genome sequence dataset, which represented 15 526 924, 12 752 646, and 7 918 591 read counts aligned to miRBase for each group (n = 3), respectively. Among these alignments, 1224, 1039, and 1029 genome-wide expressed known miRNAs accompanied by 257, 204, and 190 novel miRNAs were in BC, NS, and CAR groups, respectively. As the histological origins of paired samples undergoing NGS were identical, most of the top twenty highly expressed miRNAs in the three groups showed similar expression pattern, while rno-miR-146a-5p was found to be differentially expressed between BC and CAR groups (P < 0.001) and also between NS and CAR groups (P < 0.001). Interestingly, [Figure 3]showed that CAR treatment group had a far higher abundance of tRNAs identified (7.54%) than BC (2.60%) or NS (1.48%) groups. tRNAs can be processed to distinct small RNA species commonly referred to as tRNA-derived fragments (tRFs or tDRs). Based on the origin from 5' or 3' end, tRFs were divided into tRF-5 and tRF-3. It has been revealed that a particular tRF-3 (named CU1276 or tRF-3027) was downregulated in human malignant B-cells and lymphoma biopsies compared with naive, germinal, and memory B-cells. It interacted with argonaute (AGO) proteins, and its overexpression could repress the endogenous abundance of the tumor suppressor replication protein A1 (RPA1) in a miRNA-like manner. These results indicated that CAR treatment might cause the deregulated expression of several posttranscriptional modulators including not only miRNAs but also some tRNAs.
Furthermore, based on a “volcano plot” that simultaneously defined small P values and high fold changes, we focused on exploring miRNAs that showed significantly different expression patterns. The comparative analysis identified 84 and 70 dysregulated miRNAs between the CAR group and BC group, and between CAR group and NS group, respectively. Notably, of the top twenty differentially upregulated or downregulated miRNAs in CAR versus BC and CAR versus NS comparisons, 14 miRNAs were overlapped either in upregulated or downregulated group, indicating the consistent tendencies after the injection of carrageenan.
To explore the concrete regulatory roles of the overlapped and dysregulated miRNAs in CNP, we firstly compared the expression patterns between our results and those of previous publications. As mentioned before, it has been reported that miR-146a rs2910164 C allele could compromise miR-146a expression and thereby increase the expression of nitric oxide synthase 1 (NOS1) for decreased risk of ED. In consideration of that ED was substantially increased in patients with CP, these findings were consistent with our data that miR-146a-5p/3p was upregulated in carrageenan-induced prostatitis. Besides, miR-141 was addressed to be downregulated in CP patients, again verifying our similar findings in carrageenan-induced prostatitis model [Supplementary Table 4]. Later on, we have also attempted to link our results to those reports regarding other inflammatory diseases. For example, miR-146b-5p has been reported to be highly expressed in inflamed temporal artery biopsies from patients with giant cell arteritis; it was also significantly elevated in CNP tissues in our NGS data. miR-511-3p, which is associated with macrophage-mediated pro-inflammatory cytokine production and contributed to inflammation, was significantly upregulated in our investigations. A similar result was obtained for upregulated miR-155-5p in endomyocardial patients with inflammatory cardiomyopathy. Notably, miR-155-5p was regarded as a critical component in an inflammation-induced regulatory loop that controlled T-cell function and peripheral immune tolerance. Thus, upregulated miR-155-5p may promote the progression of CNP inflammation through the enhancement of peripheral immune tolerance.
Among the representative downregulated miRNAs in the CAR group, miR-139-5p was also significantly decreased in clinically advanced primary biliary cholangitis. It was also found that miR-200b was significantly downregulated in early pulmonary inflammation and fibrosis development, which was the same as its expression pattern reported in CNP. Specifically, miR-150-5p, which was decreased in enterovirus-induced inflammatory injury in central nervous system, was significantly downregulated in the CAR group. Given that extracellular vesicles released by CD4+ T helper (Th) lymphocytes such as T regulatory (Treg) cells contained enriched miR-150-5p levels to inhibit CD4+ T-cell proliferation, the decreased exocrine miR-150-5p levels in Treg cells could be one of the possible mechanisms leading to inflammation. More importantly, we have identified several new miRNAs with altered expression, including miR-1956-3p, miR-6215, miR-883-5p, and miR-1247-5p, whose roles in inflammation were largely unknown before.
Intriguingly, there were certain dysregulated miRNAs identified in the current work that showed inconsistent expression patterns with published data. For example, miR-19b-3p was upregulated in plasma from knee osteoarthritis patients and was positively correlated with disease severity. However, the expression of miR-19b-3p in CNP was decreased. The probable explanation was that miRNAs might exert distinct expression levels in plasma or local tissue; additionally, the complex pathological mechanisms and regulatory networks of miRNAs may not be uniform in different kinds of inflammatory diseases.
Following the differential expression analysis, we performed GO and KEGG bioinformatics analyses. The significantly enriched GO terms revealed the participation of target genes in several biological processes such as regulation of immune system process, immune response, immune effector process, inflammatory response, innate immune response, and activation of immune response, indicating the regulatory effects of miRNAs in inflammation progression and immune responses. KEGG pathway analysis enriched several immune-related pathways, such as “MAPK signaling pathway,” “cAMP signaling pathway,” “endocytosis pathway,” “mTOR signaling pathway,” and “FoxO signaling pathway.” Generally, MAPK signaling pathway is thought to be involved in innate immune Toll-like receptor (TLR) expression and is important in the T-cell antigen receptor (TCR) signaling negative feedback loop. The cAMP signaling network is necessary for inducing the catalytic activity of protein kinase A (PKA) in splenocytes, lymphocytes, and thymocytes, which is important for regulating innate as well as adaptive immune sensitivities. cAMP was the first identified intracellular second messenger of extracellular ligand action. Within the immune system, elevated intracellular cAMP levels generally reduce pro-inflammatory mediators and increase anti-inflammatory factor production, while among T-cells, Treg produce and respond to prostaglandin E2(PGE2), which is modulated by COX-2 and acts as an autocrine factor to increase intracellular cAMP. It has been reported that nonsteroidal anti-inflammatory drugs (NSAIDs) and COX-2 inhibitors could block PGE2 synthesis, eventually downregulating intracellular cAMP levels in T-cells, suggesting the crucial role of cAMP in maintaining the immune balance. We found in our study that cAMP mRNA expression and pro-inflammatory miR-155-5p were both upregulated in CNP after CAR treatment, this was consistent with a previous report that cAMP and miR-155-5p may modulate inflammatory process. Similarly, the enriched endocytosis pathway also has been reported to participate in enhanced major histocompatibility complex II (MHC II) presentation mediated by CD4+ T-cells. mTOR complex 1 (mTORC1)-induced glycolytic reprograming can activate human monocytes and macrophages and subsequently promote pro-inflammatory cytokine production. mTOR is a serine/threonine kinase that regulates cellular growth, proliferation, inflammation, and immune in response to various environmental stimuli. Although the concrete roles of mTOR in inflammation and immune were largely controversial, a central role for mTOR in innate immunity has been recently defined by its ability to restrain pro-inflammatory factors. It has been reported that inhibition of mTOR by rapamycin could augment inflammation and pulmonary injury through NF-κB activity enhancement. Similarly, in myeloid immune cells, mTOR inhibition was demonstrated to effectively block the anti-inflammatory effects of glucocorticoids. In our current investigation, as expected, mTOR downregulation was observed with upregulated miR-155-5p in CAR-induced CNP, which was supported by the fact that miR-155-5p could inhibit mTOR expression, together indicating that mTOR and miR-155-5p may coordinately modulate CNP through complex network involved in immune cells. Meanwhile, the FoxO pathway is considered to promote immune activity by negatively regulating the expression of immunosuppressive proteins including programmed death receptor 1 ligand (PD-L1). Thus, our results suggest that immune factors are probably involved in CNP regulation networks facilitated by miRNAs.
| Conclusion|| |
We have obtained the first data for the comprehensive characterization of miRNAs in CNP prostate tissues with NGS technology and identified several differentially expressed miRNAs that are potentially associated with prostatic inflammation progression. We have also enriched several immune-associated pathways, indicating the possible immunological regulatory functions in CNP progression. Our data provide an important platform for future investigations aiming to characterize the concrete roles of specific miRNAs and immune factors in CNP initiation, progression, and the development of effective theragnosis.
| Author Contributions|| |
CZL and LZ designed this experiment. LZ, YL, XGC, YZ, JC, LGZ, and HXD performed the research and analyzed the data. YL and ZYH wrote the manuscript, and then LZ, SF, and CZL revised the manuscript. All authors read and approved the final manuscript.
| Competing Interests|| |
All authors declare no competing interests.
| Acknowledgments|| |
We are grateful to Dr. Yu Yin at theFirst Affiliated Hospital of Anhui Medical University (AHMU) for her technical assistance in histological research. This work was supported by the National Natural Science Foundation of China (Grant No. 81630019, 31430028, 81401518, and 81470986), Anhui Provincial Institutes for Translational Medicine (Grant No. 2017ZHYX02), Cultivation Project of Young Top-Notch Talent Support from Anhui Medical University (AHMU), and Funding for Distinguished Young Scientists of theFirst Affiliated Hospital of AHMU.
Supplementary Information is linked to the online version of the paper on the Asian Journal of Andrology website.
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[Figure 1], [Figure 2], [Figure 3], [Figure 4]
[Table 1], [Table 2]