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Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats


1 Department of Animal Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, Gyeonggi-do 17546, Korea
2 National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Chungcheongbuk-do 28116, Korea
3 Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Chungcheongbuk-do 28116, Korea
4 Animal Biotechnology, Graduate School of Future Convergence Technology, Department of Animal Life Science, Institute of Genetic Engineering, Hankyong National University, Anseong, Gyeonggi-do 17579, Korea.
5 Present address of Bang-Jin Kim: Department of Cancer Biology, University of Pennsylvania, Philadelphia, PA 19104, USA

Correspondence Address:
Buom-Yong Ryu,
Department of Animal Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, Gyeonggi-do 17546, Korea

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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/aja.aja_80_18

PMID: 30319135

Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a high success rate. SSCs in juvenile rats were transduced in vivo with high titers of lentivirus harboring enhanced green fluorescent protein and mated with wild-type females to create founder rats. These founder rats expressed the transgene and passed on the transgene with an overall success rate of 50.0%. Subsequent generations of progeny from the founder rats both expressed and passed on the transgene. Thus, direct modification of SSCs in juvenile rats is an effective means of generating transgenic rats through the male germline. This technology could be adapted to larger animals, in which existing methods for gene modification are inadequate or inapplicable, resulting in the generation of transgenic animals in a variety of species.


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    -  Kim BJ
    -  Kim YH
    -  Oh MG
    -  Kim KJ
    -  Jung SE
    -  Jin JH
    -  Kim SU
    -  Min KS
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