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Sperm DNA damage has a negative effect on early embryonic development following in vitro fertilization


1 Reproductive Medicine Center, Family Planning Research Institute of Guangdong Province, Guangzhou 510060, China
2 Key Laboratory of Male Reproductive and Genetics, Family Planning Research Institute of Guangdong Province, Guangzhou 510060, China

Correspondence Address:
Wei-Wei Zheng,
Reproductive Medicine Center, Family Planning Research Institute of Guangdong Province, Guangzhou 510060, China; Key Laboratory of Male Reproductive and Genetics, Family Planning Research Institute of Guangdong Province, Guangzhou 510060, China

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Source of Support: None, Conflict of Interest: None

Sperm DNA damage is recognized as an important biomarker of male infertility. To investigate this, sperm DNA damage was assessed by the sperm chromatin dispersion (SCD) test in semen and motile spermatozoa harvested by combined density gradient centrifugation (DGC) and swim-up in 161 couples undergoing in vitro fertilization (IVF). Semen analysis and sperm DNA damage results were compared between couples who did or did not achieve pregnancy. The sperm DNA damage level was significantly different between the two groups (P < 0.05) and was negatively correlated with IVF outcomes. Logistic regression analysis confirmed that it was an independent predictor for achieving clinical pregnancy. The effects of different levels of sperm DNA damage on IVF outcomes were also compared. There were significant differences in day 3 embryo quality, blastocyst formation rate, and implantation and pregnancy rates (P < 0.05), but not in the basic fertilization rate between the two groups. Thus, sperm DNA damage as measured by the SCD appears useful for predicting the clinical pregnancy rate following IVF.


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    -  Zheng WW
    -  Song G
    -  Wang QL
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    -  Tan YM
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