Impact of antioxidants on seminal vesicles function and fertilizing potential in diabetic rats
Panagiota Tsounapi1, Masashi Honda1, Fotios Dimitriadis2, Bunya Kawamoto2, Katsuya Hikita2, Kuniyasu Muraoka2, Motoaki Saito3, Nikolaos Sofikitis3, Atsushi Takenaka3
1 Division of Urology, Department of Surgery, Tottori University School of Medicine, 36-1 Nishimachi, Yonago 683-8503, Japan
2 Department of Urology, University of Ioannina, School of Medicine, P.O. Box 1186, 45110, Ioannina, Greece
3 Department of Pharmacology, Kochi University , Kochi Medical School, Nankoku, 783-8505, Japan
Division of Urology, Department of Surgery, Tottori University School of Medicine, 36-1 Nishimachi, Yonago 683-8503
Source of Support: None, Conflict of Interest: None
Diabetes mellitus significantly affects the male reproduction and sexual function. In the present study, we investigated the diabetes-induced dysfunction of seminal vesicles (SVs) in the diabetes-rat model and the role of antioxidants. Streptozotocin-induced diabetes after 4 weeks caused smaller size of the organs, hypercontractility, histological abnormalities, increased concentrations of malondialdehyde in the serum and tissue, overexpression of oxidative stress markers, and cleaved caspase-3 as identified by immunohistochemistry in the SVs. In addition, diabetes resulted in deceased levels of serum testosterone and no newborns after the mating studies. Antioxidants significantly normalized all the above parameters, except for the severely decreased serum testosterone levels and the negative outcome of the mating studies. The present study gives evidence for the important role of diabetes-induced oxidative stress in the function and structure of these androgen-dependent organs. Antioxidants may be a promising supplementary therapy for diabetic male patients to alleviate ejaculatory disorders but alone is not efficient treatment for the mitigation of infertility.