ORIGINAL ARTICLE
Year : 2018  |  Volume : 20  |  Issue : 6  |  Page : 539-544

Region-specific microRNA signatures in the human epididymis


1 Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, OH 44106, USA
2 Human Molecular Genetics Program, Lurie Children's Research Center, Chicago, IL 60614, USA
3 Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
4 Section of Urology, University of Chicago Medical Center, Chicago, IL 60611, USA

Correspondence Address:
Ann Harris
Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, OH 44106, USA; Human Molecular Genetics Program, Lurie Children’s Research Center, Chicago, IL 60614, USA; Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA

Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/aja.aja_40_18

Rights and Permissions

The epithelium of the human epididymis maintains an appropriate luminal environment for sperm maturation that is essential for male fertility. Regional expression of small noncoding RNAs such as microRNAs contributes to segment-specific gene expression and differentiated functions. MicroRNA profiles were reported in human epididymal tissues but not specifically in the epithelial cells derived from those regions. Here, we reveal miRNA signatures of primary cultures of caput, corpus, and cauda epididymis epithelial cells and of the tissues from which they were derived. We identify 324 epithelial cell-derived microRNAs and 259 tissue-derived microRNAs in the epididymis, some of which displayed regionalized expression patterns in cells and/or tissues. Caput cell-enriched miRNAs included miR-573 and miR-155. Cauda cell-enriched miRNAs included miR-1204 and miR-770. Next, we determined the gene ontology pathways associated with in silico predicted target genes of the differentially expressed miRNAs. The effect of androgen receptor stimulation on miRNA expression was also investigated. These data show novel epithelial cell-derived miRNAs that may regulate the expression of important gene networks that are responsible for the regionalized gene expression and function of the epididymis.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1205    
    Printed156    
    Emailed0    
    PDF Downloaded205    
    Comments [Add]    

Recommend this journal