Table of Contents  
INVITED RESEARCH HIGHLIGHT
Year : 2015  |  Volume : 17  |  Issue : 6  |  Page : 925-928

New therapies for relapsed castration-resistant prostate cancer based on peptide analogs of hypothalamic hormones


1 Veterans Affairs Medical Center and South Florida Veterans Affairs Foundation for Research and Education, Miami, FL 33125; Department of Pathology; Department of Medicine, Division of Hematology/Oncology; Department of Medicine, Division of Endocrinology; Department of Medicine, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA
2 Veterans Affairs Medical Center and South Florida Veterans Affairs Foundation for Research and Education, Miami, FL 33125; Department of Pathology; Department of Medicine, Division of Hematology/Oncology; University of Miami Miller School of Medicine, Miami, FL 33136, USA
3 Veterans Affairs Medical Center and South Florida Veterans Affairs Foundation for Research and Education, Miami, FL 33125; Department of Urology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, 33174, USA

Date of Web Publication26-Jun-2015

Correspondence Address:
Andrew V Schally
Veterans Affairs Medical Center and South Florida Veterans Affairs Foundation for Research and Education, Miami, FL 33125; Department of Pathology; Department of Medicine, Division of Hematology/Oncology; Department of Medicine, Division of Endocrinology; Department of Medicine, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136
USA
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1008-682X.152819

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  Abstract 

It is a pleasure to contribute our presentation at the International Prostate Forum of the Annual Meeting of the American Urological Association (AUA) to this special issue of the Asian Journal of Andrology.


How to cite this article:
Schally AV, Block NL, Rick FG. New therapies for relapsed castration-resistant prostate cancer based on peptide analogs of hypothalamic hormones. Asian J Androl 2015;17:925-8

How to cite this URL:
Schally AV, Block NL, Rick FG. New therapies for relapsed castration-resistant prostate cancer based on peptide analogs of hypothalamic hormones. Asian J Androl [serial online] 2015 [cited 2020 Sep 21];17:925-8. Available from: http://www.ajandrology.com/text.asp?2015/17/6/925/152819 - DOI: 10.4103/1008-682X.152819

This article is based on a presentation delivered on the International Prostate Forum at the Annual Meeting of the American Urological Association, Orlando, Fl, USA, May 18, 2014.


We are gratified that the method developed in our laboratories [1] based on agonistic analogs of hypothalamic luteinizing hormone-releasing hormone (LHRH), also called gonadotropin-releasing hormone, which was discovered and characterized by one of us (AVS) in the 1970s, [2],[3],[4],[5],[6] has been used since the early 1980s for the treatment of hormone-dependent prostate cancer. [7],[8],[9],[10],[11] Since that time our laboratory has been trying to develop therapies for relapsed androgen-independent, castration-resistant prostate cancer (CRPC). These new therapies are also based on various analogs of hypothalamic hormones and are sorely needed when the patients with hormone-dependent prostate cancer undergo loss of effect of androgen deprivation therapy and relapse. New drugs such as abiraterone, enzalutamide (MDV 3100), cabazitaxel, sipuleucel-T, zoledronic acid, and radium-223 [12],[13],[14],[15],[16],[17] are welcome additions to the oncological-urological armamentarium but after an initial response, resistance to most of these agents appears to develop.

Consequently, in our view, synthetic antagonistic analogs of peptides such as growth hormone-releasing hormone (GHRH), LHRH, bombesin/gastrin releasing peptide (BN/GRP) that inhibit growth factors such as epidermal growth factor, vascular endothelial growth factor, insulin-like growth factor-I, II or analogs with cytotoxic moieties, which can target receptors on tumors, must be developed. [8],[9],[10],[18],[19],[20]

As we know, therapies for metastatic hormone-sensitive prostate cancer, as introduced by Huggins and Hodges [21] and others include: orchiectomy, estrogens, adrenalectomy, corticosteroids, hypophysectomy, and anti-androgens. Each can induce palliation in advanced prostate cancer; however, each has its pros and cons, morbidity and mortality, and limited long-term efficacy. Our laboratory many years ago introduced LHRH agonists, which in chronic application downregulate pituitary LHRH receptors. In turn, this downregulation of LHRH receptors leads to an inhibition of the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), producing a suppression of androgen and estrogen levels in blood. [1],[7],[8],[9],[10],[22] The treatment of patients with prostate cancer has been greatly facilitated by the introduction of sustained delivery systems [10],[22] as the administration of microcapsules containing the agonist can be performed only every 3-6 months.

Following elucidation of the effects of LHRH agonists on prostate cancer and recognition of its associated effects, we turned our attention to LHRH antagonists. We and others synthesized antagonists of LHRH that block pituitary receptors for LHRH and produce an immediate cessation of secretion of LH, FSH and sex steroids. [8],[9],[10] Early LHRH antagonists had some in edematogenic activities due to histamine release. In the LHRH antagonist, cetrorelix, developed by us, these activities were greatly reduced, and it was possible to carry out clinical studies in patients with prostate cancer and benign prostatic hyperplasia. [8],[9],[10],[23] Modern LHRH antagonist, degarelix, developed by Ferring, is the current drug of choice in this category. [24],[25],[26],[27] Degarelix has no serious adverse effects and avoids the flare phenomenon of LHRH agonists. Degarelix provides fast and effective control of testosterone, prostate specific antigen, LH, and FSH levels. It produces lower FSH levels than leuprolide, eliminates microsurges of testosterone, and leads to fewer cardiovascular and metabolic consequences than the LHRH agonists. [24],[25],[26],[27]

Luteinizing hormone-releasing hormone analogs provide effective palliative therapy for patients with advanced prostate carcinomas resulting in objective stable disease, partial remission and occasionally long-term complete remission. [8],[9] However, all hormonal therapies aimed at androgen deprivation, including orchiectomy, anti-androgens, LHRH agonists or antagonists, with or without anti-androgens, usually provide remissions of only limited duration. [8],[9] Most patients who live long enough with advanced prostatic carcinoma relapse. The prognosis of these patients with androgen-independent prostate cancer is very poor. [8],[9] The latest therapeutic drugs for prostate cancer, like abiraterone, extend the survival only by a few months. [12],[15],[16]

Continuing our studies, it became clear that some effects of LHRH analogs are exerted directly, rather than indirectly, on prostate cancer cells. [8],[9],[10],[28] We were able to demonstrate the presence of high levels of receptors for LHRH, BN/GRP, and somatostatin, as well as mRNAs for their receptor subtypes, in human prostate cancer specimens. [8],[9],[10],[28] In experimental studies on human prostate cancer lines xenografted into nude mice, analogs of LHRH, [10] analogs of somatostatin, [29] and analogs of GRP [30] each inhibited prostate tumors growth and in vitro reduced their proliferation. [8],[9],[10],[28] In vitro effects demonstrated a direct effect of these analogs on tumors. [10] Following the example with Estracyt (estramustine) consisting of nitrogen mustard coupled to carrier estrogen, we developed cytotoxic analogs of LHRH, somatostatin, and GRP which actively target respective receptors on prostate cancers. [18],[19],[20],[31],[32],[33],[34],[35],[36],[37] Thus, we have synthesized a targeted cytotoxic LHRH analog, denoted AN-152 (commercially AEZS-108, zoptarelin-doxorubicin), which combines a D-Lys-6 LHRH agonist moiety (necessary for binding to LHRH receptors on cancer cell surfaces) with the cytotoxic doxorubicin. [19] This analog delivers doxorubicin to only those cells with LHRH receptors on the cell membrane, thus avoiding cytotoxic side effects in normal cells. We found that after binding to LHRH receptors, AN-152 (AEZS-108) is internalized into the tumor cells. [18],[19],[32],[37]

AN-152 (AEZS-108) was extensively tested in vitro and also in vivo in nude mice with xenografted human cancer lines expressing LHRH receptors. [8],[9],[37] This drug inhibited the growth of a wide range of carcinomas (including kidney, prostate, urothelium) and sarcomas. These included DU-145 human androgen-independent prostate cancer as well as HT-1376, J82, RT-4 and HT-1197 bladder cancer lines. [8],[9],[37],[38],[39] The administration of AN-152 (AEZS-108) produced a powerful inhibition of tumor growth, greater than that induced by doxorubicin alone. The hybrid was found to be more efficacious and less toxic than doxorubicin. [37] These studies were followed by Phase I and II human trials in women with endometrial and ovarian cancer expressing LHRH receptors. [40],[41]

Dose escalation studies established that the maximum tolerated dose of AEZS-108 is 267 mg m[2] . Dose-limiting leukopenia and neutropenia were observed at the highest dose. [40] Liu, Pinski et al. also subjected AN-152 (AEZS-108) to Phase II trials in patients with CPRC resistant to taxane. [42] AEZS-108 is currently in Phase III trials in USA and Europe in women with endometrial cancer. We have great expectations for it.

Another class of new and important antitumor peptides that could inhibit CRPC consists of antagonists of GHRH. [43],[44],[45],[46] The story behind these compounds is most interesting. One of us (AVS) was among the investigators who discovered GHRH in hypothalami of animals in the 1960s. [43] Because only extremely small quantities of GHRH are present in the hypothalamus, it was not possible to characterize GHRH structurally until the early 1980s. [43] At that time, the clinicians became aware of cases of paraneoplastic acromegaly in some patients with pancreatic carcinoma. These tumors were actually producing GHRH, which then induced GH release from the pituitary. Thus, GHRH can be produced by tumors themselves, and it thus functions in an autocrine/paracrine fashion as a growth factor. Samples of these tumors were used for the isolation and structural elucidation of GHRH, which was then synthesized. [43],[44],[45],[46] Over the past 30 years, we have been investigating the role of GHRH in tumor growth and found that many tumors produced GHRH and had GHRH receptors. [43],[44],[45],[46] We determined that human prostate cancer specimens and human prostatic cell lines express GHRH and GHRH receptors. [47] We also demonstrated the expression of splice variants of these receptors. [47],[48],[49] This presence of GHRH receptors provides the basis for a new approach to the treatment of CRPC based on antagonists of GHRH. [8],[9],[43] Thus, over the past 20 years we produced nearly 2000 synthetic antagonistic analogs of GHRH, with each step improving their potency and half-life. [43],[44],[45],[46],[50] We substituted some of the natural (coded) L-amino acids in the N-terminal 29 amino acid sequence of GHRH that has all the biological activity either with their "D" isomers or with totally synthetic amino acids. [43],[44],[45],[46],[50] The structure of one of our GHRH antagonists, MIA-602, the one we have chosen for clinical development, is shown in [Table 1]. We have found that these GHRH antagonists can block the growth of over 20 different human tumor types, as exemplified by over 60 human cancer cell lines xenografted into nude mice. [43],[44],[45],[46],[51],[52],[53],[54],[55],[56],[57],[58],[69] We showed that MIA-602 and our other GHRH antagonists inhibited growth of PC-3 and 22Rv1 human androgen-independent prostate cancer cell lines and also hormone-dependent prostate cancer lines. [58],[59],[60],[61],[62] Our GHRH antagonists also suppressed prostate, kidney, urothelial, breast, triple-negative breast, ovary, astrocytoma, melanoma, ENT tumors, esophagus, stomach, colon, lung, adrenal cortical, pheochromocytoma, uterus, osteosarcomas and multiple lymphoma types. In addition to their inherent effects on cancer, we found that the GHRH antagonists also potentiated the effects of cytotoxic chemotherapy without enhancing the toxicity! [70]
Table 1: Structure of growth hormone-releasing hormone antagonist, MIA-602


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The side effect/toxicity profile of GHRH analogs is minimal. Similarly, LHRH agonists and antagonists also have little or no toxicity, and it is really their anti-androgenic effect, not the drugs themselves that cause some adverse effects. [71],[72]


  Perspectives for The Improvement of Therapy for Castration-Resistant Prostate Cancer Top


Novel drugs are required for the treatment of CRPC. The best option may not be the agents that target androgen receptors or compounds which inhibit enzymes involved in androgen biosynthesis. This is because the androgen deprivation created by these compounds can be overcome by mutations in androgen receptors, the appearance of splice variants of these receptors or alternate biochemical pathways.

The use of currently available cytotoxic analogs of LHRH, or somatostatin that can be targeted to prostate cancers may lead to an improvement in the treatment of CRPC and an increase in the survival rate. A new modality based on GHRH antagonists also appears to be useful for the treatment of metastatic CRPC.


  Acknowledgments Top


This material is based upon work supported in part by the Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development of the Miami VA Healthcare System; by the Departments of Pathology and Medicine, Sylvester Comprehensive Cancer Center; and Division of Hematology/Oncology of the Miller Medical School, Department of the Miller School of Medicine, University of Miami; by the South Florida Veterans Affairs Foundation for Research and Education (all to AVS); and by the L. Austin Weeks Endowment for Urologic Research (NLB). FGR received support from the Urology Care Foundation Research Scholars Program and the AUA Southeastern Section. The contents of this work do not represent the views of the Department of Veterans Affairs or the United States Government.

 
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Fahrenholtz CD, Rick FG, Garcia MI, Zarandi M, Cai RZ, et al. Preclinical efficacy of growth hormone-releasing hormone antagonists for androgen-dependent and castration-resistant human prostate cancer. Proc Natl Acad Sci U S A 2014; 111: 1084-9.  Back to cited text no. 61
    
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Pinski J, Schally AV, Groot K, Halmos G, Szepeshazi K, et al. Inhibition of growth of human osteosarcomas by antagonists of growth hormone-releasing hormone. J Natl Cancer Inst 1995; 87: 1787-94.  Back to cited text no. 62
    
63.
Kovács M, Schally AV, Hohla F, Rick FG, Pozsgai E, et al. A correlation of endocrine and anticancer effects of some antagonists of GHRH. Peptides 2010; 31: 1839-46.  Back to cited text no. 63
    
64.
Pozsgai E, Schally AV, Hocsak E, Zarandi M, Rick F, et al. The effect of a novel antagonist of growth hormone releasing hormone on cell proliferation and on the key cell signaling pathways in nine different breast cancer cell lines. Int J Oncol 2011; 39: 1025-32.  Back to cited text no. 64
    
65.
Perez R, Schally AV, Vidaurre I, Rincon R, Block NL, et al. Antagonists of growth hormone-releasing hormone suppress in vivo tumor growth and gene expression in triple negative breast cancers. Oncotarget 2012; 3: 988-97.  Back to cited text no. 65
    
66.
Jaszberenyi M, Schally AV, Block NL, Zarandi M, Cai RZ, et al. Suppression of the proliferation of human U-87 MG glioblastoma cells by new antagonists of growth hormone-releasing hormone in vivo and in vitro. Target Oncol 2013; 8: 281-90.  Back to cited text no. 66
    
67.
Seitz S, Rick FG, Schally AV, Treszl A, Hohla F, et al. Combination of GHRH antagonists and docetaxel shows experimental effectiveness for the treatment of triple-negative breast cancers. Oncol Rep 2013; 30: 413-8.  Back to cited text no. 67
    
68.
Szalontay L, Schally AV, Popovics P, Vidaurre I, Krishan A, et al. Novel GHRH antagonists suppress the growth of human malignant melanoma by restoring nuclear p27 function. Cell Cycle 2014; 13: 2790-7.  Back to cited text no. 68
    
69.
Hohla F, Winder T, Greil R, Rick FG, Block NL, et al. Targeted therapy in advanced metastatic colorectal cancer: current concepts and perspectives. World J Gastroenterol 2014; 20: 6102-12.  Back to cited text no. 69
    
70.
Rick FG, Seitz S, Schally AV, Szalontay L, Krishan A, et al. GHRH antagonist when combined with cytotoxic agents induces S-phase arrest and additive growth inhibition of human colon cancer. Cell Cycle 2012; 11: 4203-10.  Back to cited text no. 70
    
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Albertsen PC, Klotz L, Tombal B, Grady J, Olesen TK, et al. Cardiovascular morbidity associated with gonadotropin releasing hormone agonists and an antagonist. Eur Urol 2014; 65: 565-73.  Back to cited text no. 72
    



 
 
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