|Year : 2014 | Volume
| Issue : 6 | Page : 817-823
Testosterone regulates keratin 33B expression in rat penis growth through androgen receptor signaling
Yan-Min Ma1, Kai-Jie Wu2, Qiang Dang1, Qi Shi1, Yang Gao1, Peng Guo3, Shan Xu3, Xin-Yang Wang3, Da-Lin He1, Yong-Guang Gong2
1 Department of Urology, First Affiliated Hospital of Medical School, Xi'an Jiaotong University; Oncology Research Lab, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of the People's Republic of China, Xi'an 710061, China
2 Department of Urology, First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710061, China
3 Oncology Research Lab, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of the People's Republic of China, Xi'an 710061, China
|Date of Submission||19-Oct-2013|
|Date of Decision||21-Dec-2013|
|Date of Acceptance||19-Feb-2014|
|Date of Web Publication||24-Jun-2014|
Department of Urology, First Affiliated Hospital of Medical School, Xi'an Jiaotong University; Oncology Research Lab, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of the People's Republic of China, Xi'an 710061
Department of Urology, First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710061
Source of Support: None, Conflict of Interest: None
Androgen therapy is the mainstay of treatment for the hypogonadotropic hypogonadal micropenis because it obviously enhances penis growth in prepubescent microphallic patients. However, the molecular mechanisms of androgen treatment leading to penis growth are still largely unknown. To clarify this well-known phenomenon, we successfully generated a castrated male Sprague Dawley rat model at puberty followed by testosterone administration. Interestingly, compared with the control group, testosterone treatment stimulated a dose-dependent increase of penis weight, length, and width in castrated rats accompanied with a dramatic recovery of the pathological changes of the penis. Mechanistically, testosterone administration substantially increased the expression of androgen receptor (AR) protein. Increased AR protein in the penis could subsequently initiate transcription of its target genes, including keratin 33B (Krt33b). Importantly, we demonstrated that KRT33B is generally expressed in the rat penis and that most KRT33B expression is cytoplasmic. Furthermore, AR could directly modulate its expression by binding to a putative androgen response element sequence of the Krt33b promoter. Overall, this study reveals a novel mechanism facilitating penis growth after testosterone treatment in precastrated prepubescent animals, in which androgen enhances the expression of AR protein as well as its target genes, such as Krt33b.
Keywords: androgen receptor; keratin 33B; penis; testosterone
|How to cite this article:|
Ma YM, Wu KJ, Dang Q, Shi Q, Gao Y, Guo P, Xu S, Wang XY, He DL, Gong YG. Testosterone regulates keratin 33B expression in rat penis growth through androgen receptor signaling. Asian J Androl 2014;16:817-23
|How to cite this URL:|
Ma YM, Wu KJ, Dang Q, Shi Q, Gao Y, Guo P, Xu S, Wang XY, He DL, Gong YG. Testosterone regulates keratin 33B expression in rat penis growth through androgen receptor signaling. Asian J Androl [serial online] 2014 [cited 2019 Apr 22];16:817-23. Available from: http://www.ajandrology.com/text.asp?2014/16/6/817/129935 - DOI: 10.4103/1008-682X.129935
Yan-Min Ma and Kai-Jie Wu. These authors contributed equally to this work.
| Introduction|| |
Penis formation and growth are both androgens dependent, and androgen-dependent penile growth is believed to be responsible for 70%-75% of the adult penile length. , It is supposed that androgens may affect penis formation and growth in different time windows, and this effect only occurs at 3 time points: in late gestation, the first 4 years after birth, and at puberty.  Hence, androgen therapy with testosterone has been the mainstay of clinical treatment for the hypogonadotropic hypogonadal micropenis since the 1970s. Testosterone treatment usually successfully enhances penile growth in prepubescent microphallic patients, although most boys with a micropenis ultimately have a smaller than average penis in adulthood. , Overall, a normal penis length/size depends critically on androgens, but how androgens mediate this process is still unclear.
Physically, testosterone is converted into 5α-dihydrotestosterone (DHT) by the enzyme delta  -3-ketosteroid-5α-redudase (5α-reductase), which binds to a specific androgen receptor (AR) protein. , This steroid receptor complex translocates into the cell nucleus and initiates gene transcription, thus resulting in the expression of androgen target genes, which may play critical roles not only in the prostate, but also in penis growth. ,
In this study, we clearly showed that testosterone treatment resulted in a dose-dependent stimulation of penis growth using a castrated rat model and found that it dramatically induced AR protein expression with an increased penis growth. To the best of our knowledge, we are the first to apply microarrays to screen the potential genes involved in penis growth at puberty, and KRT33B was identified as a novel AR target gene during penis growth after testosterone administration. Taken together, we demonstrated that androgens are critical for penis growth during puberty through upregulation of AR protein. In addition, we discovered KRT33B, a novel AR target gene, as a new candidate gene for rat penis growth. These findings may improve the understanding of mechanisms leading to penis development, thus providing potential molecular markers for optimal androgen treatment.
| Materials and Methods|| |
Chemicals and reagents
Testosterone (25 mg ml−1 ) was purchased from Shanghai Tongyong Pharmaceutical Co. Ltd. (Shanghai, China). Rabbit polyclonal AR (N-20) and AR (N-20) X antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal KRT33B antibody (11048-1-AP) was obtained from Proteintech Group (Chicago, IL, USA). Primers for KRT33B and β-actin were designed by TaKaRa Biotechnology (Dalian, China). Horseradish peroxidase-or tetramethylrhodamine isothiocyanate-conjugated secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). The enhanced chemiluminescence (ECL) detection system was obtained from Amersham (Pittsburgh, PA, USA). The chromatin immunoprecipitation (ChIP) assay kit was purchased from Cell Signaling Technology.
All animal procedures were approved by the Institutional Animal Care and Use Committee of the Medical School of Xi'an Jiaotong University. A total of 80 males Sprague Dawley (SD) rats aged 3 weeks old and weighing 40-50 g were obtained from the Laboratory Animal Center of Xi'an Jiaotong University and randomly assigned to eight groups (n = 10 for each group). All rats except the intact controls were castrated. Briefly, the animals were anesthetized with xylazine (10 mg kg−1 ) and ketamine (90 mg kg−1 ) by intraperitoneal (ip) injection and then subjected to a midline scrotal incision, and the testicles were removed. Four days after castration, all rats except the intact and castrated controls received various amounts of testosterone propionate (0.1, 0.5, 1, 5, 10, and 50 mg kg−1 , ip, daily) for 7 days, while intact and castrated controls received vehicle only. At the end of the experiment, all animals were sacrificed to obtain the penis samples for measurements and histological assessments. Considering the inhomogeneity of the penis, we separated the body regions for molecular biology analysis ( Supplementary Figure S1). In addition, penile bones were dissolved prior to sectioning and homogenization.
Tissues were washed twice with cold phosphate-buffered saline (PBS) and then fixed immediately in 10% polyformaldehyde. Forty-eight hours after fixation, dehydration, and paraffin embedding were conducted according to standard histological protocols. Sections (5-μm each) were evaluated according to standard protocols for staining with hematoxylin-eosin (HE) or Masson's trichrome (showing fibrosis). 
Since no commercial microarray for rat has been available, until now, we generated an alternate castrated mouse model to screen the potential genes involved in penis growth at puberty. Briefly, a total of 48 pubescent (age = 21 days) and 36 adult (age = 90 days) male Chinese Kun Ming mice were castrated and randomly divided into the control (saline) or testosterone (1 mg kg−1 , ip, daily) group, respectively. Seven days after treatment, the penis samples were collected for microarray analysis. The microarray experiments were performed by KangChen Bio-tech (Shanghai, China) using a custom-made Agilent 4 × 44 k microarray, which consisted of more than 41,000 mouse genes and transcripts. Genes with a two-fold change were considered significant.
Reverse transcription polymerase chain reaction
For reverse transcription polymerase chain reaction (RT-PCR), penis samples from each group were dissected and washed twice with cold PBS. After homogenization, the total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA synthesis and PCR were performed using Superscript™ RNase H-free Reverse Transcriptase and a cDNA cycle kit (Invitrogen) in a 25-μl volume according to the manufacturer's instructions. The following primer sequences were used: Ar, 5′-CCTATCCCAGTCCCAGTTGTGTTA-3΄ (forward) and 5′-TCCACAGATCAGGCAGGTCTTC-3′ (reverse), 570 bp; Krt33b, 5′-GTGCAGATCGACAATGCCAAG-3′ (forward) and 5′-CCATTGAG-GTCTGACTCCACCA-3′ (reverse), 98 bp; β-actin, 5′-GGAGATTACTGCCC-TGGCTCCTA-3′ (forward) and 5′-GACTCATCGTACTCCTGCTTGCTG-3′ (reverse), 150 bp. The PCR conditions were as follows: 28 cycles of 95°C for 45 s, 59°C for 30 s, and 72°C for 30 s for amplification. The PCR program was initiated with a denaturation step at 95°C for 5 min and terminated with an extension step at 72°C for 5 min. The PCR products were electrophoresed on 1.5% agarose gels and analyzed using the GIS-1000 digital gel image analysis system (Shanghai, China).
Western blotting analysis
For immunoblot analyses, 30-μg samples of total protein from rat penises were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis at 4°C and 120 V for 90 min, and separated proteins were transferred onto nitrocellulose membranes for western blotting. The membranes were then blocked using Tris buffer solution with 0.05% Tween 20 containing 5% nonfat milk for 1 h at room temperature. Membranes were incubated with AR or KRT33B primary antibodies overnight at 4°C followed by peroxidase-conjugated secondary antibody for 1 h at room temperature. The bands of proteins of interest were visualized using the ECL detection system followed by exposure to X-ray film. The relative intensity of each band was determined by using Glyko BandScan software (Glyko, Novato, USA).
Indirect immunofluorescence staining
Indirect immunofluorescence staining (IIF) staining was performed on cryostat sections of a normal pubescent rat penis. Briefly, cryostat sections were fixed in acetone at − 20°C for 10 min. After rinsing in PBS, the samples were permeabilized for 1 min in PBS containing 0.1% Triton X-100. The samples were incubated with primary antibody overnight at 4°C, and then with secondary antibody for 30 min at 37°C in a water bath. Next, the sections were incubated with DAPI (0.1 μg ml−1 in PBS) for 5 min at room temperature and then covered with coverslips. At the end of each incubation interval, the sections were washed with PBS (pH 7.4) 3 times. The IIF results were documented with a photomicroscope.
siRNA and cell transfection
Ar-specific siRNA and its control were purchased from GenePharma (Shanghai, China). Transient transfection of siRNA was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), following the manufacturer's instructions. Briefly, HepG2 and MHCC-97L cells were plated in Dulbecco's modified Eagle medium containing 10% fetal bovine serum to achieve 50%-60% confluence before transfection. For each sample, 100 pmol of siRNA oligomer and 5 μl of lipofectamine 2000 Transfection Reagent were diluted in 250 μl of Opti-MEM I reduced serum medium without serum (Life Technologies, Grand Island, NY, USA), respectively. After mixing and incubation, 500 μl of oligomer-lipofectamine 2000 complexes was added to each well. The medium was changed after 4-6 h. The cells were incubated at 37°C for 48 h in a CO 2 incubator.
Chromatin immunoprecipitation assay
Chromatin immunoprecipitation assays were performed using a ChIP kit, according to the manufacturer's instructions. Briefly, the cells were cross-linked, lysed, and sonicated using a Sonic Vibra Cell VC × 130PB (Sonics and Materials, Newtown, CT, USA). After sonication, the lysate was centrifuged and 1% volume of the supernatant was diluted in 900 μl of dilution buffer. The solution was precleared with 30 μl of ChIP-Grade Protein G Agarose Beads for 2 h at 4°C with rotation. The agarose beads were pelleted, and 2% volume of the supernatant was removed for input. The remaining supernatant was immunoprecipitated with the following antibodies: 10 μg of Histone H3, 10 μg of AR (N-20) X, and 1 μg of normal rabbit IgG, overnight at 4°C with rotation. Eluted protein-DNA complexes were digested with ribonuclease A and proteinase K. DNA fragments were purified by using spin columns. Purified DNA was analyzed by standard PCR methods using SimpleChIP; Human RPL30 Extron 3 Primer and androgen response element (ARE) primer (from − 2389 to − 2232): forward, 5′-GGGACGTAGAAGAAGGAGAGAAGA-3′ and reverse, 5′-CCACTATCAGTGAGATGTGGCATG-3′. PCR products were resolved by 1.5% agarose gel electrophoresis and visualized by ethidium bromide staining.
The quantitative data were presented as mean ± standard error of the mean. Statistical analyses were carried out by analysis of variance when more than two means had to be compared, or by the Student's t-test to compare paired data. In all cases, P < 0.05 was considered as statistically significant. Statistical analyses were performed using SPSS 15.0 (SPSS Inc., Chicago, IL, USA).
| Results|| |
Androgen modulates penis growth in pubescent rats
It is well-known that androgen plays an important role in the development of the male genital system; however, the molecular mechanisms are largely unknown. Initially, we generated an animal model to investigate whether androgen could affect penis growth during puberty. Indeed, our animal study showed a dose-dependent stimulation for the penis weight, length, and width by testosterone (0-1.0 mg kg−1 ) treatment in castrated male SD rats (P < 0.05), while a higher dose of testosterone (5-50 mg kg−1 ) did not further increase these parameters ([Figure 1] a and 1b ).
|Figure 1: Effects of androgen on rat penis development. ( a ) Gross morphology of the rat penis by light microscopy with or without testosterone treatment. ( b ) Difference of the rat penis weight, length, and width after testosterone treatment. Values in each column that do not share the same letter indicate statistical significance; P < 0.05. ( c ) Histopathological assessment by H and E staining as indicated; *represents a blood vessel, represents interstitial fibrosis. Scale bars = 100 μm. ( d ) Masson's trichrome staining of rat cavernous tissue. Smooth muscle and interstitial fibrotic tissues are stained in red and blue, respectively. Scale bars = 50 μm. ( e ) Quantitative changes in smooth muscle and interstitial fibrotic tissue contents were analyzed by Image-Pro Plus 6.0 software (Media Cybernetics, Rockville, MD, USA); n = 4 for each group. *P < 0.05.|
Click here to view
In addition, HE and Masson's trichrome staining were performed to investigate the pathological changes of the penis after castration and testosterone treatment. Compared with the intact control, more hyperplastic interstitial fibrotic tissue and slit-like blood vessels were extensively observed in the corpus cavernosum of the castrated control group. After testosterone administration, the number of hyperplastic interstitial fibrotic tissues and slit-like blood vessels gradually restored to normal levels ([Figure 1] c-1e ).
Androgen increases AR protein expression in the penis
AR is known as a key factor in the androgen signaling pathway. To assess the effect of androgen on AR expression in the rat penis, western blotting and RT-PCR were performed. Our western blotting data showed that AR expression was dramatically reduced after castration and then gradually increased in a dose-dependent manner after testosterone treatment ([Figure 2] a , P < 0.05), while the RT-PCR results showed that Ar mRNA levels decreased after testosterone supplementation ([Figure 2]b). [Additional file 1] Furthermore, the IIF results clearly showed that androgen deprivation (castration) decreased AR (red color) expression in both the urethra and cavernosum and that testosterone supplementation could restore its expression ([Figure 2] c ).
|Figure 2: Ligand-dependent androgen receptor (AR) protein expression in the rat penis. Protein or mRNA levels of AR in the rat penis (n = 3-4 for each group) was determined using western blotting (a) or RT-PCR (b) with β-actin as an internal control, respectively; images are representative of three separate western blotting and PCR experiments. Quantitative analyses were carried out, and error bars represent mean ± standard error of the mean values in each column that do not share the same letter indicate statistical significance. P < 0.05. (c) Detection of AR protein expression in rat penis tissues by IIF. Cross cryostat sections of a penis probed with an anti-AR antibody (red) and DAPI (blue). Scale bars = 100 μm.|
Click here to view
Together, the results presented in [Figure 2]a-c suggested that the androgen-manipulated rat penis growth at puberty was mediated by the androgen-AR signaling pathway.
Krt33b is identified as a candidate gene for androgen-regulated penis growth
To screen the growth-associated genes in the penis that are activated by testosterone, we analyzed the gene expression profiles of both pubescent and adult mice penises after treatment with or without testosterone using an oligonucleotide microarray. A total of 734 differentially expressed transcripts showed a two-fold induction in response to testosterone in the puberty group. Among these transcripts, 33 genes were more active in response to testosterone (>4-fold change) in the puberty group, but were less active (<4-fold change) in the adult group (Supplementary Table 1). [Additional file 2] Of these genes, 6 out of the 33 were keratin or keratin associated, which indicated that these genes may be crucial for penis growth during puberty. Therefore, we selected the most responsive gene (Krt33b) for further study.
Next, using fresh penis samples, we found substantially increased Krt33b mRNA levels after testosterone administration in the puberty group by RT-PCR. These results paralleled those obtained by microarray analysis ([Figure 3]).
|Figure 3: Differential gene expression of Krt33b in the mouse penis. ( a ) Relative gene expression of the Krt33b gene in the penis of mice at puberty and adulthood based on a gene expression microarray. ( b ) RT-PCR was carried out to confirm the changes of the Krt33b gene in the penis of mice at puberty and adulthood.|
Click here to view
Krt33b is a typical AR target gene that is expressed in the rat penis
To learn about the expression characteristics of KRT33B and AR, double-labeling IIF studies using anti-KRT33B and anti-AR antibodies were carried out. Our data showed that KRT33B (red color) and AR (green color) were co-expressed in the penis urethra epithelial cells and cavernosum interstitial cells, in which most of the AR staining was nuclear but KRT33B staining was cytoplasmic ([Figure 4]). Meanwhile, IIF studies using anti-smooth muscle actin and anti-von Willebrand factor antibodies were performed to delineate the tissue architecture of the rat penis (Supplementary Figure S2).
|Figure 4: Co-expression of androgen receptor (AR) and keratin 33B (KRT33B) proteins in the rat penis by indirect immunofluorescence staining. Cross cryostat sections of a penis probed with anti-KRT33B antibody (red) and anti-AR antibody (green). Scale bars = 100 μm.|
Click here to view
Next, we extracted total proteins from penis samples from rats with various treatments and confirmed that KRT33B expression was regulated by androgen in a dose-independent manner ([Figure 5] a ). Furthermore, treatment with the anti-androgen agent flutamide (50 mg kg -1 , po, daily), which blocks the AR signaling pathway, resulted in a dramatic reduction of KRT33B expression ([Figure 5]b).
|Figure 5: Effects of androgen/AR on Krt33b expression. The levels of KRT33B after testosterone ( a ) or flutamide ( b ) administration were determined using western blotting with β-actin as an internal control; the levels of KRT33B in HepG2 ( c ) and MHCC-97L ( d ) cells at 48 h after transient transfection of AR siRNA were determined using western blotting with β-actin as an internal control; western blots are representative of three independent experiments. *P < 0.05 (NC vs siAR in each group).|
Click here to view
To investigate whether KRT33B was directly mediated by the androgen-AR signaling pathway, we selected HepG2 and MHCC-97L cells, which expressed both AR and KRT33B, for further study in vitro. Using siRNA transfection methodology, we found that the expression of KRT33B was obviously reduced by knocking down AR (P < 0.05), in both HepG2 and MHCC-97L cells ([Figure 5]c and 5d, respectively).
To clarify the mechanism of AR regulating Krt33b expression, a ChIP assay was carried out to identify the putative AREs in the KRT33B gene promoter. An approximately 2.4-kb sequence region upstream of the TATA box of the Krt33b gene is schematically shown in [Figure 6]a, and a homology screen for the semipalindromic AR consensus binding sequence GG A / T ACAnnnTGTTCT revealed the presence of one putative ARE. Interestingly, in our study, the ChIP assay clearly demonstrated that AR could directly bind onto the promoter AREs of the Krt33b gene in both HepG2 and MHCC-97L cell lines ([Figure 6] b and 6c , respectively).
Taken together, the results presented in [Figure 4], [Figure 5] and [Figure 6] implied that Krt33b gene expression could be regulated by androgen through AR directly targeting it's ARE sequence.
| Discussion|| |
Penis size among normal men varies considerably, and men's anxiety regarding the "normality" of their penis size is widespread. , Boys with a micropenis due to hypogonadotropic hypogonadism are frequently treated with testosterone to induce penis growth. , A proportion show a poor growth response, and most boys with a micropenis ultimately have a smaller than average penis in adulthood.  Recent animal studies have also identified that fetal or postnatal androgen treatment only advances the penis growth rate but does not increase its ultimate length.  Hence, besides the action of androgen, there may be other factors that play different functions to coordinate the progression of penis growth.
To understand this process, an animal model of 21-day-old castrated male SD rats exposed with testosterone was generated. Testosterone administration (0-1.0 mg kg−1 ) resulted in a dose-dependent stimulation of penis weight, length, and width increases. Consistently, hyperplastic interstitial fibrosis and aberrant slit-like blood vessels appeared after castration and were restored after testosterone supplementation. However, a higher dose of testosterone (5.0-50 mg kg−1 ) did not increase these parameters further.
In addition, we also found that the AR protein levels in the rat penis increased in a dose-dependent manner after testosterone treatment (0-1.0 mg kg−1 ), while Ar mRNA levels were decreased after testosterone administration. In fact, these inconsistent conclusions have also been reported by others. ,,, One study found that testosterone and DHT were not the major factors in the physiological downregulation of Ar in the rat corpora cavernosa.  In addition, Takane et al. have reported that DHT accelerates the decline of penile Ar mRNA levels;  while Gonzalez-Cadavid et al. have demonstrated that androgen can upregulate the levels of Ar mRNA in rat penile smooth-muscle cells.  Furthermore, Lin et al. have hypothesized that the in vivo AR downregulation in penile smooth muscle by androgen is mediated indirectly by a paracrine mechanism. Meanwhile, Zhou et al. have reported that the stabilization of AR protein requires its receptor to be occupied with androgens and that the AR NH 2 -terminal domain has a specific role in stabilization by slowing the rate of ligand dissociation and AR degradation. Therefore, in our study, we assumed that androgen may modulate AR protein expression in the rat penis through posttranscriptional regulation.
Taken together, these results imply the importance of AR modulation in the setting of hypogonadotropic hypogonadism treatment. Furthermore, in clinical practice, high-dose testosterone should not be preferred, because compared with a lower dose; such doses of testosterone bring about more side-effects but no further stimulative effect on penis growth.
As described previously, AR exerts its transcriptional activity through activating its target genes, which may execute their biological function alone or together with other factors. Interestingly, using a gene expression array, we found that Krt33b mRNA levels in the pubescent rat penis were obviously upregulated after androgen treatment. Furthermore, we provided evidence that the transcription of this gene was directly controlled by AR directly binding onto its gene promoter. Whether it is a truth or a coincidence, recent research has shown that pubic hair growth is highly relevant to penis growth.  Sexual hairs such as pubic hair, a beard, and axillary hair arise from small vellus, unmedullated hairs during puberty under the influence of circulating androgens and represent particularly coarse, strongly pigmented, and medullated hairs in adults. , According to their similar growth environment, human pubic hair and the penis seem to share a common growth mechanism.
During hair growth, numerous keratins organize into filaments to participate in the production of the hair shaft.  The keratin multigene family includes the keratins, which are expressed in various types of soft keratins, and the hard keratins, which are involved in the formation of hard-keratinized structures such as hairs, nails, claws, and beaks. ,, The best-known function of keratins and keratin filaments is to provide a scaffold for epithelial cells and tissues to sustain mechanical stress, maintain their structural integrity, ensure mechanical resilience, protect against variations in hydrostatic pressure, and establish cell polarity.  Previous studies have confirmed that some keratins can be regulated by androgens. Jave-Suarez et al. have shown that the expression of human hair keratin 7 is directly regulated by androgens. Similarly, Yoshida et al. also have reported that androgen regulates hair keratin 37 expression in the human occipital hair medulla. In the present study, we found that KRT33B was generally expressed in urethra epithelial cells and cavernosum interstitial cells of the rat penis, and its expression was modulated by androgen. Meanwhile, one limitation of this study was that we did not separate the penile urethra from the corpus cavernosum for analyses.
| Conclusion|| |
The present study initially provided evidence that androgen-induced KRT33B is generally expressed in the rat penis and its gene transcription is directly controlled by AR. Therefore, we hypothesized that KRT33B may participate in the progression of penis growth during puberty. Thus, advanced biological techniques should be applied to investigate the potential functions of this gene in the future.
| Author Contributions|| |
YMM and KJW participated in the design of the trial, conducted the data acquisition, interpreted and analyzed the data, and drafted and revised the manuscript. QD and QS designed the study and contributed the study materials. YG, PG, SX, and XYW conducted the data acquisition and analyzed the data. YGG and DLH designed the trial, interpreted the data, and drafted the manuscript. All authors read and approved the final manuscript.
| Competing Interests|| |
The authors declare that they have no competing financial interests.
| Acknowledgments|| |
This study was partially supported by the National Natural Science Foundation of China (NSFC 81270688 to YGG).
| References|| |
|1.||Boas M, Boisen KA, Virtanen HE, Kaleva M, Suomi AM, et al. Postnatal penile length and growth rate correlate to serum testosterone levels: a longitudinal study of 1962 normal boys. Eur J Endocrinol 2006; 154: 125-9. |
|2.||Camurdan AD, Oz MO, Ilhan MN, Camurdan OM, Sahin F, et al. Current stretched penile length: cross-sectional study of 1040 healthy Turkish children aged 0 to 5 years. Urology 2007; 70: 572-5. |
|3.||Husmann DA. Micropenis: an animal model and its human correlates. Adv Exp Med Biol 2002; 511: 41-54. |
|4.||Bin-Abbas B, Conte FA, Grumbach MM, Kaplan SL. Congenital hypogonadotropic hypogonadism and micropenis: effect of testosterone treatment on adult penile size why sex reversal is not indicated. J Pediatr 1999; 134: 579-83. |
|5.||Tietjen DN, Uramoto GY, Tindall DJ, Husmann DA. Micropenis in hypogonadotropic hypogonadism: response of the penile androgen receptor to testosterone treatment. J Urol 1998; 160: 1054-7. |
|6.||Zhang YL, Zhou ZX, Zhang YD, Parker MG. Expression of androgen receptors and prostatic steroid-binding protein during development of the rat ventral prostate. J Endocrinol 1988; 117: 361-6. |
|7.||Chang C, Saltzman A, Yeh S, Young W, Keller E, et al. Androgen receptor: an overview. Crit Rev Eukaryot Gene Expr 1995; 5: 97-125. |
|8.||Gonzalez-Cadavid NF, Swerdloff RS, Lemmi CA, Rajfer J. Expression of the androgen receptor gene in rat penile tissue and cells during sexual maturation. Endocrinology 1991; 129: 1671-8. |
|9.||Tindall DJ, Chang CH, Lobl TJ, Rowley DR. Molecular properties of the androgen receptor in rat ventral prostate. Ann N Y Acad Sci 1984; 438: 39-53. |
|10.||Tomada I, Tomada N, Almeida H, Neves D. Androgen depletion in humans leads to cavernous tissue reorganization and upregulation of Sirt1-eNOS axis. Age (Dordr) 2013; 35: 35-47. |
|11.||Ponchietti R, Mondaini N, Bonafè M, Di Loro F, Biscioni S, et al. Penile length and circumference: a study on 3,300 young Italian males. Eur Urol 2001; 39: 183-6. |
|12.||Vardi Y. Is penile enlargement an ethical procedure for patients with a normal-sized penis? Eur Urol 2006; 49: 609-11. |
|13.||Husmann DA, Cain MP. Microphallus: eventual phallic size is dependent on the timing of androgen administration. J Urol 1994; 152: 734-9. |
|14.||Welsh M, MacLeod DJ, Walker M, Smith LB, Sharpe RM. Critical androgen-sensitive periods of rat penis and clitoris development. Int J Androl 2010; 33: e144-52. |
|15.||Shen R, Lin MC, Sadeghi F, Swerdloff RS, Rajfer J, et al. Androgens are not major down-regulators of androgen receptor levels during growth of the immature rat penis. J Steroid Biochem Mol Biol 1996; 57: 301-13. |
|16.||Takane KK, George FW, Wilson JD. Androgen receptor of rat penis is down-regulated by androgen. Am J Physiol 1990; 258: E46-50. |
|17.||González-Cadavid N, Vernet D, Fuentes Navarro A, Rodríguez JA, Swerdloff RS, et al. Up-regulation of the levels of androgen receptor and its mRNA by androgens in smooth-muscle cells from rat penis. Mol Cell Endocrinol 1993; 90: 219-29. |
|18.||Lin MC, Rajfer J, Swerdloff RS, González-Cadavid NF. Testosterone down-regulates the levels of androgen receptor mRNA in smooth muscle cells from the rat corpora cavernosa via aromatization to estrogens. J Steroid Biochem Mol Biol 1993; 45: 333-43. |
|19.||Zhou ZX, Lane MV, Kemppainen JA, French FS, Wilson EM. Specificity of ligand-dependent androgen receptor stabilization: receptor domain interactions influence ligand dissociation and receptor stability. Mol Endocrinol 1995; 9: 208-18. |
|20.||Soydan H, Akyol Ý, Ates F, Yilmaz O, Dursun F, et al. Cross-sectional analysis of penile length in males 13 to 15 years old according to pubertal development stages. J Urol 2012; 188: 1319-23. |
|21.||Randall VA. Androgens and human hair growth. Clin Endocrinol (Oxf) 1994; 40: 439-57. |
|22.||Inui S, Itami S. Androgen actions on the human hair follicle: perspectives. Exp Dermatol 2013; 22: 168-71. |
|23.||Thibaut S, Collin C, Langbein L, Schweizer J, Gautier B, et al. Hair keratin pattern in human hair follicles grown in vitro. Exp Dermatol 2003; 12: 160-4. |
|24.||Lynch MH, O'Guin WM, Hardy C, Mak L, Sun TT. Acidic and basic hair/nail ("hard") keratins: their colocalization in upper cortical and cuticle cells of the human hair follicle and their relationship to "soft" keratins. J Cell Biol 1986; 103: 2593-606. |
|25.||Heid HW, Werner E, Franke WW. The complement of native alpha-keratin polypeptides of hair-forming cells: a subset of eight polypeptides that differ from epithelial cytokeratins. Differentiation 1986; 32: 101-19. |
|26.||McLean WH, Moore CB. Keratin disorders: from gene to therapy. Hum Mol Genet 2011; 20: R189-97. |
|27.||Bragulla HH, Homberger DG. Structure and functions of keratin proteins in simple, stratified, keratinized and cornified epithelia. J Anat 2009; 214: 516-59. |
|28.||Jave-Suarez LF, Langbein L, Winter H, Praetzel S, Rogers MA, et al. Androgen regulation of the human hair follicle: the type I hair keratin hHa7 is a direct target gene in trichocytes. J Invest Dermatol 2004; 122: 555-64. |
|29.||Yoshida H, Taguchi H, Kitahara T, Takema Y, Visscher MO, et al. Keratins of the human occipital hair medulla: androgenic regulation of in vitro hair keratin K37 expression. Br J Dermatol 2013; 169: 218-21. |
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]