Table of Contents  
ORIGINAL ARTICLE
Year : 2014  |  Volume : 16  |  Issue : 2  |  Page : 295-304

Metabolic syndrome and prostate abnormalities in male subjects of infertile couples


1 Sexual Medicine and Andrology Unit, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy
2 Sexual Medicine and Andrology Unit, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence; Endocrinology Unit, Maggiore-Bellaria Hospital, Bologna, Italy
3 Department of Urology, Careggi Hospital, University of Florence, Florence, Italy

Date of Submission17-May-2013
Date of Decision05-Jul-2013
Date of Acceptance19-Jul-2013
Date of Web Publication07-Jan-2014

Correspondence Address:
Mario Maggi
Sexual Medicine and Andrology Unit, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence
Italy
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1008-682X.122341

Rights and Permissions
  Abstract 

No previous study has evaluated systematically the relationship between metabolic syndrome (MetS) and prostate-related symptoms and signs in young infertile men. We studied 171 (36.5 ± 8.3-years-old) males of infertile couples. MetS was defined based on the National Cholesterol Education Program Third Adult Treatment Panel. All men underwent hormonal (including total testosterone (TT) and insulin), seminal (including interleukin-8 (IL-8), seminal plasma IL-8 (sIL-8)), scrotal and transrectal ultrasound evaluations. Because we have previously assessed correlations between MetS and scrotal parameters in a larger cohort of infertile men, here, we focused on transrectal features. Prostate-related symptoms were assessed using the National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) and the International Prostate Symptom Score (IPSS). Twenty-two subjects fulfilled MetS criteria. In an age-adjusted logistic ordinal model, insulin levels increased as a function of MetS components (Wald = 29.5, P < 0.0001) and showed an inverse correlation with TT (adjusted r = -0.359, P< 0.0001). No association between MetS and NIH-CPSI or IPSS scores was observed. In an age-, TT-, insulin-adjusted logistic ordinal model, an increase in number of MetS components correlated negatively with normal sperm morphology (Wald = 5.59, P< 0.02) and positively with sIL-8 levels (Wald = 4.32, P < 0.05), which is a marker of prostate inflammation, with prostate total and transitional zone volume assessed using ultrasound (Wald = 17.6 and 12.5, both P < 0.0001), with arterial peak systolic velocity (Wald = 9.57, P = 0.002), with texture nonhomogeneity (hazard ratio (HR) = 1.87 (1.05-3.33), P < 0.05), with calcification size (Wald = 3.11, P< 0.05), but not with parameters of seminal vesicle size or function. In conclusion, in males of infertile couples, MetS is positively associated with prostate enlargement, biochemical (sIL8) and ultrasound-derived signs of prostate inflammation but not with prostate-related symptoms, which suggests that MetS is a trigger for a subclinical, early-onset form of benign prostatic hyperplasia.

Keywords: infertile men; interleukin-8; metabolic syndrome; prostate-related symptoms and signs; semen analysis; transrectal ultrasound


How to cite this article:
Lotti F, Corona G, Vignozzi L, Rossi M, Maseroli E, Cipriani S, Gacci M, Forti G, Maggi M. Metabolic syndrome and prostate abnormalities in male subjects of infertile couples. Asian J Androl 2014;16:295-304

How to cite this URL:
Lotti F, Corona G, Vignozzi L, Rossi M, Maseroli E, Cipriani S, Gacci M, Forti G, Maggi M. Metabolic syndrome and prostate abnormalities in male subjects of infertile couples. Asian J Androl [serial online] 2014 [cited 2019 Jun 17];16:295-304. Available from: http://www.ajandrology.com/text.asp?2014/16/2/295/122341 - DOI: 10.4103/1008-682X.122341


  Introduction Top


Metabolic syndrome (MetS) is a diagnostic category based on a cluster of abnormalities (abdominal obesity, impaired glucose metabolism, hypertriglyceridemia, reduced high-density lipoprotein (HDL) cholesterol and hypertension) that identifies subjects at high risk for diabetes and cardiovascular diseases. [1],[2],[3],[4] Various diagnostic criteria for MetS have been proposed over recent years. Indeed, the parameters to be used for the diagnosis of MetS and their thresholds remains under debate. [4],[5] Nonetheless, insulin resistance has been recognized as the common feature underlying MetS, [6] with visceral adiposity being the driving force. [2],[3],[4],[5] In addition to diabetes and cardiovascular diseases, several other pathologic conditions are associated with MetS including nonalcoholic fatty liver disease, polycystic ovarian syndrome, obstructive sleep apnea and lipodystrophy. [2] In addition, in the male, hypogonadism, erectile dysfunction and psychological disturbances are often associated with MetS. [4],[7],[8],[9]

A possible association between MetS and male infertility has also been hypothesized. [1] Although several studies have evaluated the impact of being overweight and obesity on male reproductive health, [10],[11],[12],[13] only a few studies have assessed the influence of some MetS components on male fertility [1] and none have considered MetS to be a diagnostic category. We evaluated this topic specifically in a cohort of 351 males of infertile couples without known genetic abnormalities. [9] Even after adjusting for confounders, we noted that an increase in the number of MetS components associated with hypogonadism, poor sperm morphology (but not other sperm parameters), testis ultrasound nonhomogeneity, erectile dysfunction, somatization (expression of physical symptoms in the absence of medically explained physical illness) and depressive traits. [9]

In addition, in this last decade, a growing body of evidence has also documented an independent association between benign prostatic hyperplasia (BPH)/lower urinary tract symptoms (LUTS) and obesity/MetS. [14],[15],[16],[17],[18] In particular, we previously reported a positive correlation between MetS and BPH-related chronic inflammation in patients undergoing surgery for BPH. [19],[20] A recently published epidemiological survey of the Boston area (BACH) confirmed an association between MetS and LUTS; however, when subjects were stratified by age, the association was confirmed only in the youngest individuals. [21]

This study is aimed at investigating systematically the possible associations between MetS and prostate-related symptoms and signs in a cohort of young men in infertile unions and to establish whether these associations correlate with fertility.


  Materials and Methods Top


We retrospectively evaluated a consecutive series of 187 male patients (age: 36.5 ± 8.3 years) who attended our Outpatient Clinic initially between January 2010 and December 2011 seeking medical care for infertility. Based on guidelines of the World Health Organization, infertility was defined as the inability of a sexually active couple to achieve pregnancy despite unprotected intercourse over a period of greater than 12 months. [22] Subjects with karyotypic abnormalities (n = 3), chromosome Y microdeletions (n = 2) or an absence of at least one vas deferens and/or one seminal vesicle (n = 11) were excluded from the analysis. Hence, a cohort of 171 selected patients was used for the analyses. The sociodemographic and clinical phenotype of the sample population is summarized in [Table 1].
Table 1: Clinical and transrectal color Doppler ultrasound characteristics of the sample

Click here to view


All patients were evaluated prior to any treatment. All enrolled patients underwent the typical diagnostic protocol used for infertility in newly referred subjects at the Andrology Outpatient Clinic. They underwent a complete andrological and physical examination and blood pressure (mean of three measurements taken 5 min apart in the sitting position using a standard sphygmomanometer), height, weight and waist circumference were measured. In addition, routine scrotal and transrectal ultrasounds were performed because our regional healthcare system does not allow any genetic analysis on infertile patients unless a suspected obstruction has been evaluated. All data were collected as part of the routine clinical procedure; therefore, based on Italian law, approval from the local Ethical Committee was not required. In addition, at the time of the initial visit, all patients provided written, informed consent to have their clinical records included in a dedicated database to be used, anonymously, for clinical research purposes.

MetS assessment

MetS was defined, based on the National Cholesterol Education Program Third Adult Treatment Panel, [26] as the presence of three or more of the following five factors: central obesity (waist circumference >102 cm), elevated triglycerides (≥1.7 mmol l -1 or treated for elevated triglycerides), elevated blood pressure (systolic blood pressure ≥130 mmHg and/or diastolic blood pressure ≥85 mmHg or treated for hypertension), elevated fasting glucose (≥6.1 mmol l -1 or treated for diabetes) and reduced HDL cholesterol (<1.03 mmol l -1 or treated for dyslipidemia).

Biochemical parameters

Blood samples were drawn in the morning after an overnight fast to determine blood glucose (using the glucose oxidase method; Aeroset Abbott, Rome, Italy), HDL cholesterol and triglycerides (using the automated enzymatic colorimetric method; Aeroset Abbott, Rome, Italy), total testosterone (TT, using the electrochemiluminescent method; Modular Roche, Milan, Italy), insulin levels and sex hormone binding globulin (SHBG) using an electrochemiluminescence immunoassay (Roche Diagnostics, Mannheim, Germany). Free testosterone was calculated based on Vermeulen's formula ( available at http://www.issam.ch/freetesto.htm ). [27]

Semen analyses and determination of seminal plasma interleukin 8 (sIL-8) levels

On the same day as the ultrasound, all patients underwent semen analysis, which was performed based on World Health Organization criteria. [28] In addition, routine urine and seminal cultures were assessed in all men. Furthermore, sIL-8, a reliable surrogate marker of prostatitis, [29] was also quantified. Seminal plasma aliquots were frozen and stored for later quantification of sIL-8 levels using conventional two-site enzyme-linked immunosorbent assay (ELISA; human IL-8 ELISA set; BD Biosciences, San Diego, CA, USA) according to the manufacturer's instructions. [29] Each seminal plasma sample was diluted from 1:5 to 1:625. Assay sensitivity for sIL-8 was <1 pg ml -1 .

Screening of prostate-related symptoms and lower urinary tract symptoms

Patients were asked to complete the Italian translation of the National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI), [30] which is a brief self-reported questionnaire for screening prostatitis symptoms and that scores for pain, voiding symptoms and quality of life. The NIH-CPSI total score was calculated as the sum of the scores of these domains. LUTS were evaluated using the Italian translation of the International Prostate Symptom Score (IPSS), which is a brief self-administered questionnaire for screening symptoms related to BPH and that comprises seven questions on symptoms and one question on quality of life. [31]

Transrectal color Doppler ultrasonography (CDU)

We previously reported an association between MetS and scrotal parameters in a larger cohort of subjects who attended our unit for infertility. [9] The characteristics of that cohort were not different from the cohort in this study; thus, in this study, we focused on the possible associations between MetS and transrectal ultrasound features.

All patients underwent scrotal and transrectal CDU; the latter before and after ejaculation. To prevent bias on the part of the examiner, scrotal and transrectal CDU was performed intermittently by two experienced physicians who were unaware of the clinical data and who used the same ultrasonographic console (Hitachi H21, Hitachi Medical System, Tokyo, Japan). Prostate and seminal vesicle CDU features were studied by scanning the organs at 5 mm intervals at various longitudinal, transverse and oblique scans based on previous studies [23],[24],[25],[32],[33] using a transrectal biplanar probe (linear transducer U533L 7.5 MHz; convex transducer U533C 6.5 MHz) and an 'end fire' probe (V53W 6.5 MHz, field of view 50°-200°). Based on: 1) anteroposterior (APD) and 2) transverse (TD) diameters at the maximal dimensions and 3) the superior-inferior or longitudinal diameter (LD) at the maximal length from the base to the apex of the midline sagittal plane, prostate volume was calculated using the formula of the ellipsoid (APD × TD × LD × π/6) based on previous publications. [23],[34],[35] During this exam, similar methods were also used to determine the transitional zone volume (TZV) based on a previous publication. [35] Prostate echogenicity was defined based on previous studies. [23],[24],[32],[33] The severity of prostate texture nonhomogeneity was classified using an arbitrary Likert scale and scores of 0-3 (0 = homogeneous texture, 1 = mild, 2 = moderate and 3 = severe texture nonhomogeneity). Prostate vascularization, prostate hyperemia and arterial prostatic peak systolic velocity (APPSV) were defined based on previous studies [23],[24],[36],[37] and evaluated prior to ejaculation to avoid post-ejaculatory changes in vascular flow pattern as has been previously reported. [38] Seminal vesicle ultrasound features and abnormalities were defined based on previous studies. [23],[25],[33],[39],[40] Ejaculatory duct CDU characteristics were evaluated after ejaculation to better emphasize indirect CDU signs of subobstruction. [23],[25],[39]

Scrotal CDU was performed systematically using a 7.5 MHz high-frequency linear probe (L54M 6-13 MHz) at various longitudinal, transverse and oblique scans with patients lying in a supine position. [23],[32],[33] Testicular and epididymal CDU features were examined based on previous studies. [23],[32],[33]

Identification of case patients and controls

MetS was defined as described above. Subjects with ≥3 MetS components (n = 22) were compared with controls selected from the same cohort at a 1:2 ratio (n = 44). For each case, the first two patients following those with MetS within the same series who were the same age (±4 years) and who showed a similar TT level (± 4 nmol l−1 ), smoking habit (current/nonsmoker) and moderate-severe alcohol consumption (current/no consumption of ≥4 drinks per day based on a previous publication [41] ). For statistical analyses that compared cases with age-, TT-, smoking habit-, moderate-severe alcohol consumption-matched controls, associations with P < 0.05 were considered significant.

Data analyses

Data were expressed as the mean ± s.d. when normally distributed, the median (quartiles) for parameters with non-normal distributions and as percentages when categorical. Correlations were assessed using Spearman's or Pearson's methods as appropriate. Differences between more than two groups were assessed using one-way analysis of variances. Unpaired two-sided Student's t-test was used to compare means of normally distributed parameters. Relative risks and 95% confidence intervals were calculated for correlations of categorical parameters, and chi-squared tests were used for comparisons. Stepwise multiple linear, logistic binary or ordinal regressions were applied for multivariate analyses as appropriate. All statistical analyses were performed in SPSS (Statistical Package for Social Sciences, Chicago, USA) for Windows 20.0.


  Results Top


Among the 171 patients studied (age: 36.6 ± 8.4 years), 44.4% (n = 76) showed no components of MetS, whereas one, two, three, four and five MetS factors were present in 47 (27.5%), 26 (15.2%), 16 (9.4%), three (1.75%) and three (1.75%) subjects, respectively. Twenty-two subjects (12.9%) fulfilled the criteria of National Cholesterol Education Program Third Adult Treatment Panel MetS. Subjects with MetS were older (43.8 ± 10.6 vs 35.5 ± 7.5 years for MetS and no-MetS subjects, respectively; P < 0.0001). No difference in the percentage of subjects who smoked currently or consumed moderate-severe amounts of alcohol was found when MetS and no-MetS subjects were compared (21.1% vs 28.6%, P = 0.496; 16.7% vs 24.8%, P = 0.233; MetS vs no-MetS subjects, respectively). In addition, no difference in the prevalence of leukocytospermia or current positive urine and/or seminal cultures was observed when men with and without MetS were compared (5.3% vs 8.8%, P = 0.508; 5.6% vs 8.1%, P = 0.575; MetS vs non-MetS subjects, respectively).

Correlations of MetS with hormonal, clinical and semen parameters

Age-adjusted logistic ordinal models showed that insulin levels increased as a function of MetS components (Wald = 29.5 (0.09-0.18), P < 0.0001) [Figure 1]a. [Figure 1]b-d also showed the age-adjusted relationships between insulin and TT, SHBG and calculated free testosterone: all of these parameters decreased as a function of increasing insulin levels (adjusted r = -0.359, P < 0.0001; adjusted r = -0.200, P < 0.001; adjusted r = -0.320, P < 0.0001, respectively). In view of these associations, all the following analyses were adjusted for age, insulin and TT levels.
Figure 1: Association between insulin levels and the number of metabolic syndrome (MetS) components (National Cholesterol Education Program Third Adult Treatment Panel classifi cation) (a), total testosterone (b), sex hormone binding globulin (c) and calculated free testosterone levels (d). In (a), subjects
with no, one or more MetS components are indicated. In (b– d), subjects with or without MetS are shown as fi lled or empty dots, respectively.


Click here to view


Digitorectal examinations revealed that an enlarged prostate was positively associated with the number of MetS components (hazard ratio (HR) =1.47 (1.03-2.14)) for each increment in the number of MetS components, P < 0.05). Of the semen parameters, only normal semen morphology was negatively associated with an increasing number of MetS components (Wald = 5.59 (-0.15 to -0.01), P < 0.02).

No association between MetS components and other physical or seminal parameters including leukocytospermia was observed (data not shown). Finally, no association between MetS components and current positive urine and/or seminal cultures was detected (data not shown).

Correlations of MetS with prostate-related symptoms and signs (sIL-8)

No association was found between MetS and prostate-related symptoms as captured by both NIH-CPSI and IPSS (not shown). A stepwise, positive correlation between the number of MetS components and sIL-8 levels was observed (Wald = 4.32 (0.04-1.48), P < 0.05) [Figure 2]a. In particular, among the MetS components, only waist circumference was positively associated with sIL-8 [Figure 2]b.
Figure 2: Association between metabolic syndrome (MetS) and seminal interleukin 8 (sIL-8) levels or prostate volume at ultrasound. Association between the number of MetS components (NCEP-ATPIII classifi cation) and sIL-8 levels (a) or prostate volume at ultrasound (c). The number of subjects with no, one or more MetS components is indicated. Hazard ratio (95% confi dence interval) for sIL-8 levels (b) and prostate volume at ultrasound (d) as detected by iterative logistic regression analyses considering MetS components as putative predictors. MetS components are defined by abnormal parameters or by specific therapy (t) based on NCEP-ATPIII groupings. HDL, high density lipoprotein.

Click here to view


Correlations of MetS with transrectal ultrasound parameters

A progressively higher prostate volume was detected by ultrasound as a function of an increasing number of MetS components (Wald = 17.6 (0.05-0.13), P < 0.0001) [Figure 2]c. In a logistic iterative analysis, of the MetS factors, waist size and reduced HDL cholesterol were significantly associated with prostate volume [Figure 2]d. Interestingly, TZV was positively correlated with total prostate volume even after adjusting for the aforementioned confounders (adjusted r = 0.757, P < 0.0001). Thus, TZV also increased as a function of an increasing number of MetS components (Wald = 12.5 (0.07-0.24), P < 0.0001). In addition, TZV of MetS subjects was significantly higher than other subjects (10.7 ± 10.8 vs 3.9 ± 2.6 ml; P < 0.0001; subjects with MetS vs those without MetS, respectively). Finally, similarly to total prostate volume results, TZV was significantly associated with reduced HDL cholesterol levels (HR = 1.15 (1.01-1.31), P < 0.05). Conversely, only a trend towards statistical significance was observed for increased waist (HR = 1.11 (0.99-1.24), P = 0.08).

A positive association between an increasing number of MetS components was also observed for some ultrasonographic prostate features, such as arterial peak systolic velocity (APPSV, Wald = 9.57 (0.05-0.24), P = 0.002) [Figure 3]a and diameter of the major calcification (Wald = 3.11 (0.01-0.13), P < 0.05) [Figure 3]c. Using a binary logistic model, moderate-severe prostate texture nonhomogeneity was also associated with an increase in the number of MetS components (HR = 1.87 (1.05-3.33) for each increment in the number of MetS components, P < 0.05) [Figure 3]c. Of the MetS components, only increased waist circumference was significantly associated with APPSV [Figure 3]b and with moderate-severe texture nonhomogeneity [Figure 3]d. After adjusting for confounders, no associations between MetS related-prostate CDU abnormalities and standard semen parameters were observed [Table 2]. Finally, no associations between the number of MetS components and seminal vesicle features or the mean diameter of the deferential ampulla were observed (data not shown).
Figure 3: Association between metabolic syndrome (MetS) and arterial prostatic peak systolic velocity (APPSV), texture nonhomogeneity and major calcification size of the prostate as evaluated using color Doppler ultrasound (CDU). Association between the number of metabolic syndrome (MetS) components (NCEP-ATPIII classifi cation) and APPSV (a), moderate-severe nonhomogeneity prevalence (c) or major calcification size (c, inset) of the prostate as evaluated using CDU. The number of subjects with no, one or more MetS components is indicated. Hazard ratio (95% confi dence interval) for APPSV (b) and prostate moderate-severe nonhomogeneity (d) as detected by iterative logistic regression analysis considering MetS components as putative predictors. MetS components are defi ned by abnormal parameters or by specifi c therapy (t) based on NCEP-ATPIII groupings. m-s, moderate-severe.

Click here to view
Table 2: Univariate associations between MetS-related prostate color Doppler ultrasound characteristics and semen parameters

Click here to view


Case-control analyses

Correlations between MetS and seminal or ultrasound parameters showing statistical significance were further assessed by comparing subjects with MetS with matched controls (matched for age, TT, smoking habit and moderate-severe alcohol consumption) [Table 3] at a 1:2 ratio. Even in the case-controlled analyses, subjects with three or more MetS components showed a lower percentage of normal sperm morphology, higher sIL8 levels and more frequent prostate abnormalities, such as greater volume, higher arterial peak systolic velocity, greater calcification size and prevalence of moderate-severe nonhomogeneity [Table 3]. No difference in the prevalence of leukocytospermia or current positive urine and/or seminal cultures was observed when MetS subjects were compared with controls [Table 3].
Table 3: Comparisons between subjects with metabolic syndrome and 1:2 ratio-matched controls (matched for age, total testosterone, smoking habits and moderate-severe alcohol consumption)

Click here to view


Correlations of insulin levels with clinical, seminal and transrectal ultrasound parameters

Univariate analyses revealed positive associations between insulin levels and increased prostate volume detected by either digitorectal examination (RR = 1.08 (1.03-1.14), P = 0.002 for each insulin mU l -1 increment) or ultrasound (r = 0.294, P < 0.0001), moderate-severe texture nonhomogeneity (RR = 1.08 (1.00-1.17), P = 0.05 for each insulin mU l -1 increment), hyperemia (RR = 1.05 (1.00-1.11), P < 0.05 for each insulin mU l -1 increment) and arterial peak systolic velocity (r = 0.286, P < 0.0001) and a negative correlation with the prostatic venous plexus diameter (r = -0.180, P < 0.02). When a multivariate regression model was applied that included age and TT, the significant associations between insulin levels and prostate volume detected both by digitorectal examination (HR = 1.07 (1.01-1.13), P < 0.05 for each insulin mUl -1 increment) and ultrasound (adjusted r = 0.327, P < 0.0001) [Figure 4]a and arterial peak systolic velocity (adjusted r = 0.229, P = 0.002) [Figure 4]b were confirmed. However, when the number of MetS components was introduced into the same model, only prostate volume detected by ultrasound was significantly associated with insulin levels (adjusted r = 0.171, P < 0.05).
Figure 4: Associations between insulin levels and prostate volume (a) or arterial prostatic peak systolic velocity (b) evaluated using color Doppler ultrasound (CDU). Subjects with or without metabolic syndrome are shown as filled or empty dots, respectively

Click here to view


Even after adjusting for the aforementioned confounders, no associations between insulin levels and semen parameters, sIL-8 levels, seminal vesicle features or diameters of the deferential ampulla were observed (data not shown).


  Discussion Top


This study demonstrates that in a cohort of relatively young male subjects examined for infertility, a stepwise, component-dependent association was observed between an increase in MetS severity and prostate enlargement and/or inflammatory signs (including sIL-8 levels and CDU abnormalities), but not with current infection of the male genital tract. No association between MetS-related prostate CDU abnormalities and semen parameters was detected. However, in this cohort, MetS was associated with poor sperm morphology. Reduced HDL levels and increased abdominal adiposity were the main correlates of prostate enlargement in this young, asymptomatic cohort.

The association between MetS and prostate enlargement is consistent with several previous reports. [20],[42],[43],[44],[45],[46],[47],[48],[49] Thus far, only a few studies have examined relatively young adults, and they offer conflicting results. [50],[51] Here, we report a novel association in a relatively young population (mean age 36.6 ± 8.4 years) of males of infertile couples. Increased central obesity and reduced HDL cholesterol were the parameters that most closely correlated with prostate enlargement. A potential relationship between BPH/prostate enlargement and obesity or increased waist circumference has been widely reported in several, [14],[52] but not all previous studies. [20] Moreover, low HDL cholesterol has been previously reported as a risk factor for the development of BPH. [20],[42],[43],[44],[45] Our data suggest that MetS, and particularly high waist circumference and reduced HDL cholesterol, may play an important role in prostate growth onset at a young age.

We also noted a significant, stepwise correlation between the number of MetS components and seminal IL-8 (sIL-8), which has been proposed as a surrogate marker of prostate inflammation. [29],[53],[54],[55] IL-8 is a proinflammatory chemokine that is secreted by several cell types and that contributes to inflammation by acting in concert with IL-1β and IL-6. [56],[57],[58] Higher IL-8 levels have been reported in the expressed prostatic secretions of subjects with BPH, bacterial prostatitis and chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). [59],[60],[61] Because IL-8 in seminal plasma is considerably higher when compared with serum levels, [62],[63] local (within the male genital tract) production has been suggested. [55],[59],[63] Of different cytokines and chemokines, sIL-8 appears to be the most reliable and predictive surrogate marker of prostatitis, [29],[54] and it is associated with CDU features suggestive of prostate inflammation in patients with male accessory gland infection (MAGI). [23] IL-8 is actively involved in BPH-associated chronic inflammation and mediates epithelial and stromal cell proliferation. [54] In BPH tissues, epithelial and stromal cells secrete IL-8 actively [29],[64] in response to varying stimuli including the proinflammatory cytokines interferon (IFN) γ and IL-17 that are produced by prostate-infiltrating Th1 and Th17 cells, respectively. [53],[65],[66],[67] In particular, human stromal prostatic cells actively contribute to the organ-specific inflammatory process by acting as targets of bacterial or viral toll-like receptors agonists and as antigen-presenting cells capable of activating antigen-specific CD4 + T cells. [68] In BPH cells, toll-like receptor activation leads to the production of proinflammatory cytokines (IL-6) and chemokines (IL-8 and CXCL10) capable of recruiting CXCR1 and CXCR2-positive leukocytes and CD15 + neutrophils. [29] Finally, IL-8 stimulates overgrowth of prostate stromal and epithelial cells by directly promoting the proliferation of senescent epithelial cells, [69] stromal transdifferentiation of myofibroblasts [70] and by increasing secretion of fibroblast growth factor 2. [71] Hence, IL-8 appears to be the link between T cell-mediated inflammatory responses and cell proliferation in the pathogenesis of BPH. [54],[67]

A higher prevalence of MetS components was also associated with other CDU features of prostate inflammation including texture nonhomogeneity, major calcification size and elevated APPSV. Increased waist size is the common determinant of all of these CDU abnormalities. Prostate nonhomogeneity is typically considered a CDU abnormality related to inflammation [32] and has been previously associated with elevated sIL-8 levels in infertile subjects with MAGI. [23] Moreover, prostatic hyperechogenicity, which is associated with areas of calcification, has been previously proposed to be a CDU feature suggestive of MAGI. [33] In particular, prostatic high-density echoes are considered the sonographic correlates of prostatic calculi and corpora amylacea, which as confirmed by histology performed on ultrasound-guided biopsies of the prostate. [72] A recent report indicated that prostatic calculi and corpora amylacea comprised acute inflammatory proteins including lactoferrin, calprotectin, myeloperoxidase and α-defensins, all of which are in neutrophil granules. [73] Prostatic calcifications are common in patients with CP and have been associated with the maintenance or enhancement of prostate inflammation, bacterial colonization and duration of symptoms. [74],[75] A positive correlation between sIL-8 levels and calcification size has also been previously reported by our group. [23] Finally, we noted that APPSV correlated with MetS severity. Arterial PSV reflects tissue inflammation at various sites including the thyroid, [76],[77] exocrine glands [78],[79] and synovial membrane/joints. [80],[81] Thus far, APPSV has been studied for varying purposes including evaluation of BPH, [82] prostate cancer, [83] varicocele-related prostate CDU changes [84] and premature ejaculation. [37] More recently, elevated APPSV has been proposed as a CDU parameter that correlates with prostate inflammation, [23],[24],[37] and in the prostate, APPSV is closely related to sIL-8. [23]

No correlation was found between the number of MetS components and current positive urine and/or seminal cultures suggesting that MetS is not associated with current infection of the male genital tract but is rather associated with chronic inflammation.

The relationship between central obesity and dyslipidemia with prostate overgrowth and inflammation, even in young subjects, is the main finding of this study. We previously reported a clear-cut association between MetS severity and prostate size [20] and inflammation [19],[85] in cohorts of aged subjects. In the study of Gacci et al., [20] reduced HDL cholesterol and increased triglyceride levels were also noted to be the main determinants of MetS-related prostate alterations. However, previous studies were performed in old individuals undergoing surgery for BPH. [19],[85] By culturing BPH stromal cells obtained from those patients, we demonstrated that in addition to tumor necrosis factor-α and lipopolysaccharide, oxidized LDL was capable of increasing IL-8, IL-6 and basic fibroblast growth factor secretion. [19] In addition, tumor necrosis factor-α sensitized BPH cells to oxidized LDL by inducing its receptor (LOX-1) [19] . In a rabbit model of MetS we recently demonstrated that a 3-month high-cholesterol diet (HFD) induced severe prostatic inflammation characterized by increased corpora amylacea, fibrosis and hypoxia. [86] In addition, the mRNA expression of several proinflammatory cytokines including IL-8 and T lymphocyte, macrophage, neutrophil and fibrosis/myofibroblast activation markers were significantly increased in the prostate of HFD animals. [86] Together, all of these data suggest that BPH may be viewed as a complex disorder that also involves a metabolic component that may begin early in the life of the male, and although asymptomatic, it is likely detectable even in the early stages of the disease as suggested by this study. The mechanisms underpinning the relationship between MetS and prostate inflammation are likely to be similar in young and old men, but chronic exposure to elevated inflammation may contribute to BPH in the long-term.

Because hyperinsulinemia and insulin resistance represent the cornerstone of all definitions of MetS, [3],[6] all of the data reported here were adjusted for insulin levels. When the specific contribution of hyperinsulinemia was considered, after adjusting for MetS components and TT levels and age, we observed a specific effect of increased insulin levels only on prostate volume and not on prostate inflammation. This finding is in apparent contrast with results published recently by our group that showed that insulin increased IL-8 release from myofibroblastic hBPH cells. [19] However, in that study, the effect of insulin was negligible when compared with oxidized LDL (sixfold lower). [19] The growth-promoting activity of insulin on the prostate gland is well-documented in several experimental and epidemiological studies. [87]

In this study, no association between an increase in the number of MetS components and prostate-related symptoms was observed, using either NIH-CPSI or IPSS scores. The lack of correlation between MetS and LUTS is consistent with some studies, but contrasts with most previous studies. [15],[16],[18] However, all previous studies were performed in aged cohorts; whereas, our data were obtained from young subjects with relatively small prostates as assessed by ultrasound.

Finally, even after adjusting for confounders including the hypogonadal status, we observed an association between the number of MetS components and poor sperm morphology. These results confirmed previous findings obtained using a larger cohort of males of infertile couples where we assessed the possible correlations of MetS with scrotal parameters. [9] The possible impact of MetS on sperm morphology was discussed in detail in that study. Here, we extend our investigation to possible associations between MetS-related prostate CDU features and semen parameters and note no correlation. This finding suggests that the effect of MetS on sperm quality is independent of MetS-related prostate abnormalities. Although a possible association between CP or MAGI and sperm quality has been proposed and some prostate CDU features have been proposed as suggestive of MAGI, [33],[88],[89],[90] a specific association between prostate CDU features and sperm parameters alterations has not been demonstrated. Here, we report that a number of MetS components, but not related prostate CDU abnormalities, are associated with poor sperm morphology.

This study has several limitations. First, the results were derived from patients consulting an Italian Andrology Clinic for infertility, and our population could have different characteristics from the general male population or those males consulting general practitioners for reasons other than infertility. Second, a true control group comprising age-matched, apparently healthy, fertile men is lacking and therefore true normative data on sonographic parameters cannot be inferred. Third, the data cannot distinguish between men who are clinically infertile and those who are not, and whether this feature confounds the results is unknown. Fourth, this study relies on a relatively small and young sample. Fifth, markers of prostate inflammation other than sIL-8 are lacking. Furthermore, parallel mechanistic studies to determine the consequences of IL-8 secretion on prostate growth and differentiation are warranted. Finally, the link between MetS or insulin levels and prostate enlargement in this study are correlative only. Statistically significant associations in a cross-sectional study do not infer causality.

However, this study also has several strengths. First, this study systematically evaluates several hormonal, seminal, laboratory and ultrasound parameters in a consecutive series of males of infertile couples. Second, during the same sonographic session, this study examined both scrotal and transrectal ultrasound features before and after ejaculation. Third, this study was performed on a sample of relatively young, infertile men and investigated a population that is poorly studied in the scientific literature. Fourth, this study considers several possible confounders, such as age, testosterone and insulin levels, smoking habits, alcohol consumption, leukocytospermia and positive semen and urine cultures of the patients. Fifth, a statistical analysis comparing patients with MetS with age-, TT-, smoking habits- and alcohol consumption-matched controls was performed. Finally, the study examined several end points simultaneously within the same population, which enabled a valid comparison of the coprevalence of the examined parameters and supported their possible association with the number of MetS components.


  Conclusions Top


This study demonstrates that in a cohort of men with infertility, a component-dependent, stepwise association was observed between an increase in the number of MetS components and the total and transitional zone prostate enlargement and prostate related-inflammatory signs, but not symptoms or current infection of the male genital tract, which suggests a subclinical inflammation of the prostate. Relative prostate overgrowth may also correlate with MetS-related hyperinsulinemic state. In addition, MetS but not MetS's related prostate CDU abnormalities was associated with poor sperm morphology.


  Author Contributions Top


FL, GC and MM conceived and designed the study, performed the statistical analysis and drafted the manuscript. LV, MG and GF participated in the design of the study. FL, EM, MR and SC participated in data collection and management. All authors read and approved the final manuscript.


  Competing Interests Top


The authors declare no competing interests.

 
  References Top

1.Kasturi SS, Tannir J, Brannigan RE. The metabolic syndrome and male infertility. J Androl 2008; 29: 251-9.  Back to cited text no. 1
    
2.Cornier MA, Dabelea D, Hernandez TL, Lindstrom RC, Steig AJ, et al. The metabolic syndrome. Endocr Rev 2008; 29: 777-822.  Back to cited text no. 2
    
3.Eckel RH, Alberti KG, Grundy SM, Zimmet PZ. The metabolic syndrome. Lancet 2010; 375: 181-3.  Back to cited text no. 3
    
4.Corona G, Rastrelli G, Vignozzi L, Mannucci E, Maggi M. Testosterone, cardiovascular disease and the metabolic syndrome. Best Pract Res Clin Endocrinol Metab 2011; 25: 337-53.  Back to cited text no. 4
    
5.Corona G, Mannucci E, Forti G, Maggi M. Hypogonadism, ED, metabolic syndrome and obesity: a pathological link supporting cardiovascular diseases. Int J Androl 2009; 32: 587-98.  Back to cited text no. 5
    
6.Reaven P. Metabolic syndrome. J Insur Med 2004; 36: 132-42.  Back to cited text no. 6
[PUBMED]    
7.Corona G, Mannucci E, Schulman C, Petrone L, Mansani R, et al. Psychobiologic correlates of the metabolic syndrome and associated sexual dysfunction. Eur Urol 2006; 50: 595-604.  Back to cited text no. 7
    
8.Corona G, Rastrelli G, Morelli A, Vignozzi L, Mannucci E, et al. Hypogonadism and metabolic syndrome. J Endocrinol Invest 2011; 34: 557-67.  Back to cited text no. 8
    
9.Lotti F, Corona G, Degli Innocenti S, Filimberti E, Scognamiglio V, et al. Seminal, ultrasound and psychobiological parameters correlate with metabolic syndrome in male members of infertile couples. Andrology 2013; 1: 229-39  Back to cited text no. 9
    
10.Hammoud AO, Gibson M, Peterson CM, Meikle AW, Carrell DT. Impact of male obesity on infertility: a critical review of the current literature. Fertil Steril 2008; 90: 897-904.  Back to cited text no. 10
    
11.MacDonald AA, Herbison GP, Showell M, Farquhar CM. The impact of body mass index on semen parameters and reproductive hormones in human males: a systematic review with meta-analysis. Hum Reprod Update 2010; 16: 293-311.  Back to cited text no. 11
    
12.Sermondade N, Faure C, Fezeu L, Lévy R, Czernichow S, et al. Obesity and increased risk for oligozoospermia and azoospermia. Arch Intern Med 2012; 172: 440-2.  Back to cited text no. 12
    
13.Sermondade N, Faure C, Fezeu L, Shayeb AG, Bonde JP, et al. BMI in relation to sperm count: an updated systematic review and collaborative meta-analysis. Hum Reprod Update 2013; 19: 221-31  Back to cited text no. 13
    
14.Mongiu AK, McVary KT. Lower urinary tract symptoms, benign prostatic hyperplasia, and obesity. Curr Urol Rep 2009; 10: 247-53.  Back to cited text no. 14
    
15.Moul S, McVary KT. Lower urinary tract symptoms, obesity and the metabolic syndrome. Curr Opin Urol 2010; 20: 7-12.  Back to cited text no. 15
    
16.Gorbachinsky I, Akpinar H, Assimos DG. Metabolic syndrome and urologic diseases. Rev Urol 2010; 12: 157-80.  Back to cited text no. 16
    
17.Parsons JK. Lifestyle factors, benign prostatic hyperplasia, and lower urinary tract symptoms. Curr Opin Urol 2011; 21: 1-4.  Back to cited text no. 17
[PUBMED]    
18.De Nunzio C, Aronson W, Freedland SJ, Giovannucci E, Parsons JK. The correlation between metabolic syndrome and prostatic diseases. Eur Urol 2012; 61: 560-70.  Back to cited text no. 18
    
19.Vignozzi L, Gacci M, Cellai I, Santi R, Corona G, et al. Fat boosts, while androgen receptor activation counteracts, BPH-associated prostate inflammation. Prostate 2013; 73: 789-800.  Back to cited text no. 19
    
20.Gacci M, Vignozzi L, Sebastianelli A, Salvi M, Giannessi C, et al. Metabolic syndrome and lower urinary tract symptoms: the role of inflammation. Prostate Cancer Prostatic Dis 2013; 16: 101-6.  Back to cited text no. 20
    
21.Kupelian V, McVary KT, Kaplan SA, Hall SA, Link CL, et al. Association of lower urinary tract symptoms and the metabolic syndrome: results from the Boston area community health survey. J Urol 2013; 189: S107-14.  Back to cited text no. 21
    
22.World Health Organization. WHO manual for the standardized investigation and diagnosis of the infertile couple. Cambridge: Cambridge University Press; 2000.  Back to cited text no. 22
    
23.Lotti F, Corona G, Mancini M, Filimberti E, Degli Innocenti S, et al. Ultrasonographic and clinical correlates of seminal plasma interleukin-8 levels in patients attending an andrology clinic for infertility. Int J Androl 2011; 34: 600-13.  Back to cited text no. 23
    
24.Lotti F, Corona G, Colpi GM, Filimberti E, Degli Innocenti S, et al. Elevated body mass index correlates with higher seminal plasma interleukin 8 levels and ultrasonographic abnormalities of the prostate in men attending an andrology clinic for infertility. J Endocrinol Invest 2011; 34: 336-42.  Back to cited text no. 24
    
25.Lotti F, Corona G, Colpi G, Filimberti E, Degli Innocenti S, et al. Seminal vesicles ultrasound features in a cohort of infertility patients. Hum Reprod 2012; 27: 974-82.  Back to cited text no. 25
    
26.Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. Executive summary of the third report of The National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel III). JAMA 2001; 285: 2486-97.  Back to cited text no. 26
[PUBMED]    
27.Vermeulen A, Verdonck L, Kaufman JM. A critical evaluation of simple methods for the estimation of free testosterone in serum. J Clin Endocrinol Metab 1999; 84: 3666-72.  Back to cited text no. 27
    
28.World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5 th ed. WHO press; 2010.  Back to cited text no. 28
    
29.Penna G, Mondaini N, Amuchastegui S, Degli Innocenti S, Carini M, et al. Seminal plasma cytokines and chemokines in prostate inflammation: interleukin 8 as a predictive biomarker in chronic prostatitis/chronic pelvic pain syndrome and benign prostatic hyperplasia. Eur Urol 2007; 51: 524-33.  Back to cited text no. 29
    
30.Litwin MS, McNaughton-Collins M, Fowler Jr FJ, Nickel JC, Calhoun EA, et al. The National Institutes of Health chronic prostatitis symptom index: development and validation of a new outcome measure. Chronic Prostatitis Collaborative Research network. J Urol 1999; 162: 369-75.  Back to cited text no. 30
    
31.Barry MJ, Fowler FJ Jr, O'Leary MP, Bruskewitz RC, Holtgrewe HL, et al. The American Urological Association symptom index for benign prostatic hyperplasia. The Measurement Committee of the American Urological Association. J Urol 1992; 148: 1549-57.  Back to cited text no. 31
    
32.Behre HM, Kliesch S, Schädel F, Nieschlag E. Clinical relevance of scrotal and transrectal ultrasonography in andrological patients. Int J Androl 1995; 18: 27-31.  Back to cited text no. 32
    
33.Vicari E. Seminal leukocyte concentration and related specific reactive oxygen species production in patients with male accessory gland infections. Hum Reprod 1999; 14: 2025-30.  Back to cited text no. 33
[PUBMED]    
34.Collins GN, Raab GM, Hehir M, King B, Garraway WM. Reproducibility and observer variability of transrectal ultrasound measurements of prostatic volume. Ultrasound Med Biol 1995; 21: 1101-5.  Back to cited text no. 34
    
35.St Sauver JL, Jacobson DJ, Girman CJ, McGree ME, Lieber MM, et al. Correlations between longitudinal changes in transitional zone volume and measures of benign prostatic hyperplasia in a population-based cohort. Eur Urol 2006; 50: 105-11.  Back to cited text no. 35
    
36.Cho IR, Keener TS, Nghiem HV, Winter T, Krieger JN. Prostate blood flow characteristics in the chronic prostatitis/pelvic pain syndrome. J Urol 2000; 163: 1130-3.  Back to cited text no. 36
    
37.Lotti F, Corona G, Rastrelli G, Forti G, Jannini EA, et al. Clinical correlates of erectile dysfunction and premature ejaculation in men with couple infertility. J Sex Med 2012; 9: 2698-707.  Back to cited text no. 37
    
38.Keener TS, Winter TC, Berger R, Krieger JN, Nodell C, et al. Prostate vascular flow: the effect of ejaculation as revealed on transrectal power Doppler sonography. AJR Am J Roentgenol 2000; 175: 1169-72.  Back to cited text no. 38
    
39.Colpi GM, Negri L, Nappi RE, Chinea B. Is transrectal ultrasonography a reliable diagnostic approach in ejaculatory duct sub-obstruction? Hum Reprod 1997; 12: 2186-91.  Back to cited text no. 39
    
40.Lotti F, Corona G, Maseroli E, Rossi M, Silverii A, et al. Clinical implications of measuring prolactin levels in males of infertile couples. Andrology 2013; 1: 764-71.  Back to cited text no. 40
    
41.Boddi V, Corona G, Monami M, Fisher AD, Bandini E, et al. Priapus is happier with Venus than with Bacchus. J Sex Med 2010; 7: 2831-41.  Back to cited text no. 41
    
42.Hammarsten J, Högstedt B, Holthuis N, Mellström D. Components of the metabolic syndrome-risk factors for the development of benign prostatic hyperplasia. Prostate Cancer Prostatic Dis 1998; 1: 157-62.  Back to cited text no. 42
    
43.Hammarsten J, Högstedt B. Clinical, anthropometric, metabolic and insulin profile of men with fast annual growth rates of benign prostatic hyperplasia. Blood Press 1999; 8: 29-36.  Back to cited text no. 43
    
44.Hammarsten J, Högstedt B. Hyperinsulinaemia as a risk factor for developing benign prostatic hyperplasia. Eur Urol 2001; 39: 151-8.  Back to cited text no. 44
    
45.Ozden C, Ozdal OL, Urgancioglu G, Koyuncu H, Gokkaya S, et al. The correlation between metabolic syndrome and prostatic growth in patients with benign prostatic hyperplasia. Eur Urol 2007; 51: 199-203.  Back to cited text no. 45
    
46.Sohn JC, Chang HS, Kim CI. The correlation between metabolic syndrome and the prostate volume. Korean J Urol 2007; 48: 603-7.  Back to cited text no. 46
    
47.Park JS, Park JK. The meaning of metabolic syndrome X in patients suffering with benign prostatic hyperplasia. Korean J Urol 2007; 48: 696-700.  Back to cited text no. 47
    
48.Koo KC, Cho KS, Kang EM, Kwon SW, Hong SJ. The relationship between metabolic syndrome and prostate volume in men over sixties who underwent prostate health check-up. Korean J Urol 2008; 49: 813-7.  Back to cited text no. 48
    
49.Byun HK, Sung YH, Kim W, Jung JH, Song J, et al. Relationships between Prostate-Specific Antigen, Prostate Volume, and Components of Metabolic Syndrome in Healthy Korean Men. Korean J Urol 2012; 53: 774-8.  Back to cited text no. 49
    
50.Jeong JH, Kim ET, Kim DK. Association of metabolic syndrome and benign prostate enlargement in young korean males. Korean J Urol 2011; 52: 757-62.  Back to cited text no. 50
    
51.Yim SJ, Cho YS, Joo KJ. Relationship between metabolic syndrome and prostate volume in Korean men under 50 years of age. Korean J Urol 2011; 52: 390-5.  Back to cited text no. 51
    
52.Parsons JK, Sarma AV, McVary K, Wei JT. Obesity and benign prostatic hyperplasia: clinical connections, emerging etiological paradigms and future directions. J Urol 2013; 189: S102-6.  Back to cited text no. 52
    
53.Penna G, Fibbi B, Amuchastegui S, Corsiero E, Laverny G, et al. The vitamin D receptor agonist elocalcitol inhibits IL-8-dependent benign prostatic hyperplasia stromal cell proliferation and inflammatory response by targeting the RhoA/Rho kinase and NF-kappaB pathways. Prostate 2009; 69: 480-93.  Back to cited text no. 53
    
54.Fibbi B, Penna G, Morelli A, Adorini L, Maggi M. Chronic inflammation in the pathogenesis of benign prostatic hyperplasia. Int J Andr 2009; 32: 1-15.  Back to cited text no. 54
    
55.Lotti F, Maggi M. Interleukin 8 and the male genital tract. J Reprod Immunol 2013; 100: 54-65.  Back to cited text no. 55
    
56.Steiner GE, Djavan B, Kramer G, Handisurya A, Newman M, et al. The picture of the prostatic lymphokine network is becoming increasingly complex. Rev Urol 2002; 4: 171-7.  Back to cited text no. 56
    
57.Baggiolini M, Loetscher P, Moser B. Interleukin-8 and the chemokine family. Int J Immunopharmacol 1995; 17: 103-8.  Back to cited text no. 57
    
58.Feldmann M, Saklatvala J. Proinflammatory cytokines. In: Oppenheim JJ, Feldmann M, editors. Cytokine Reference. New York: academic Press; 2001. p. 291-305.  Back to cited text no. 58
    
59.Hochreiter WW, Nadler RB, Koch AE, Campbell PL, Ludwig M, et al. Evaluation of the cytokines interleukin 8 and epithelial neutrophil activating peptide 78 as indicators of inflammation in prostatic secretions. Urology 2000; 56: 1025-9.  Back to cited text no. 59
    
60.Orhan I, Onur R, Ilhan N, Ardiçoglu A. Seminal plasma cytokine levels in the diagnosis of chronic pelvic pain syndrome. Intl J Urol 2001; 8: 495-9.  Back to cited text no. 60
    
61.Liu L, Li Q, Han P, Li X, Zeng H, et al. Evaluation of interleukin-8 in expressed prostatic secretion as a reliable biomarker of inflammation in benign prostatic hyperplasia. Urology 2009; 74: 340-4.  Back to cited text no. 61
    
62.Shimoya K, Matsuzaki N, Tsutsui T, Taniguchi T, Saji F, et al. Detection of interleukin-8 (IL-8) in seminal plasma and elevated IL-8 in seminal plasma of infertile patients with leukospermia. Fertil Steril 1993; 5: 885-8.  Back to cited text no. 62
    
63.Koumantakis E, Matalliotakis I, Kyriakou D, Fragouli Y, Relakis K. Increased levels of interleukin-8 in human seminal plasma. Andrologia 1998; 30: 339-43.  Back to cited text no. 63
    
64.Konig JE, Senge T, Allhoff EP, Konig W. Analysis of the inflammatory network in benign prostate hyperplasia and prostate cancer. Prostate 2004; 58: 121-9.  Back to cited text no. 64
    
65.Steiner GE, Stix U, Handisurya A, Willheim M, Haitel A, et al. Cytokine expression pattern in benign prostatic hyperplasia infiltrating T cells and impact of lymphocytic infiltration on cytokine mRNA profile in prostatic tissue. Lab Invest 2003; 83: 1131-46.  Back to cited text no. 65
    
66.Kramer G, Mitteregger D, Marberger M. Is benign prostatic hyperplasia (BPH) an immune inflammatory disease? Eur Urol 2007; 51: 1202-16.  Back to cited text no. 66
    
67.Adorini L, Penna G, Fibbi B, Maggi M. Vitamin D receptor agonists target static, dynamic, and inflammatory components of benign prostatic hyperplasia. Ann N Y Acad Sci 2010; 1193: 146-52.  Back to cited text no. 67
    
68.Penna G, Fibbi B, Amuchastegui S, Cossetti C, Aquilano F, et al. Human benign prostatic hyperplasia stromal cells as inducers and targets of chronic immuno-mediated inflammation. J Immunol 2009; 182: 4056-64.  Back to cited text no. 68
    
69.Castro P, Xia C, Gomez L, Lamb DJ, Ittmann M. Interleukin-8 expression is increased in senescent prostatic epithelial cells and promotes the development of benign prostatic hyperplasia. Prostate 2004; 60: 153-9.  Back to cited text no. 69
    
70.Schauer IG, Ressler SJ, Tuxhorn JA, Dang TD, Rowley DR. Elevated epithelial expression of interleukin-8 correlates with myofibroblast reactive stroma in benign prostatic hyperplasia. Urology 2008; 72: 205-13.  Back to cited text no. 70
    
71.Giri D, Ittmann M. Interleukin-8 is a paracrine inducer of fibroblast growth factor 2, a stromal and epithelial growth factor in benign prostatic hyperplasia. Am J Pathol 2001; 159: 139-47.  Back to cited text no. 71
    
72.Doble A, Carter SS. Ultrasonographic findings in prostatitis. Urol Clin North Am 1989; 16: 763-72.  Back to cited text no. 72
    
73.Sfanos KS, Wilson BA, De Marzo AM, Isaacs WB. Acute inflammatory proteins constitute the organic matrix of prostatic corpora amylacea and calculi in men with prostate cancer. Proc Natl Acad Sci U S A 2009; 106: 3443-8.  Back to cited text no. 73
    
74.Meares EM Jr. Infection stones of prostate gland. Laboratory diagnosis and clinical management. Urology 1974; 4: 560-6.  Back to cited text no. 74
[PUBMED]    
75.Shoskes DA, Lee CT, Murphy D, Kefer J, Wood HM. Incidence and significance of prostatic stones in men with chronic prostatitis/chronic pelvic pain syndrome. Urology 2007; 70: 235-8.  Back to cited text no. 75
    
76.Lagalla R, Caruso G, Finazzo M. Monitoring treatment response with colour and power Doppler. Eur J Radiol 1998; 27: 149-56.  Back to cited text no. 76
    
77.Corona G, Biagini C, Rotondi M, Bonamano A, Cremonini N, et al. Correlation between, clinical, biochemical, colour-Doppler ultrasound thyroid parameters, and CXCL-10 in autoimmune thyroid diseases. Endocrine J 2008; 55: 345-50.  Back to cited text no. 77
    
78.Giovagnorio F, Pace F, Giorgi A. Sonography of lacrimal glands in Sjögren syndrome. J Ultrasound Med 2000; 19: 505-9.  Back to cited text no. 78
    
79.Carotti M, Salaffi F, Manganelli P, Aralia G. Ultrasonography and colour doppler sonography of salivary glands in primary Sjögren's sindrome. Clin Rheumatol 2001; 20: 213-9.  Back to cited text no. 79
    
80.Varsamidis K, Varsamidou E, Tjetjis V, Mavropoulos G. Doppler sonography in assessing disease activity in rheumatoid arthritis. Ultrasound Med Biol 2005; 31: 739-43.  Back to cited text no. 80
    
81.Carotti M, Salaffi F, Morbiducci J, Ciapetti A, Bartolucci L, et al. Colour Doppler ultrasonography evaluation of vascularization in the wrist and finger joints in rheumatoid arthritis patients and healthy subjects. Eur J Radiol 2012; 81: 1834-8.  Back to cited text no. 81
    
82.Berger AP, Horninger W, Bektic J, Pelzer A, Spranger R, et al. Vascular resistance in the prostate evaluated by colour Doppler ultrasonography: is benign prostatic hyperplasia a vascular disease? BJU Int 2006; 98: 587-90.  Back to cited text no. 82
    
83.Turgut AT, Olçücüoglu E, Koºar P, Geyik PO, Koºar U, et al. Power Doppler ultrasonography of the feeding arteries of the prostate gland: a novel approach to the diagnosis of prostate cancer? J Ultrasound Med 2007; 26: 875-83.  Back to cited text no. 83
    
84.Lotti F, Corona G, Mancini M, Biagini C, Colpi GM, et al. The association between varicocele, premature ejaculation and prostatitis symptoms: possible mechanisms. J Sex Med 2009; 6: 2878-87.  Back to cited text no. 84
    
85.Vignozzi L, Cellai I, Santi R, Lombardelli L, Morelli A, et al. Antiinflammatory effect of androgen receptor activation in human benign prostatic hyperplasia cells. J Endocrinol 2012; 214: 31-43.  Back to cited text no. 85
    
86.Vignozzi L, Morelli A, Sarchielli E, Comeglio P, Filippi S, et al. Testosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbit. J Endocrinol 2012; 212: 71-84.  Back to cited text no. 86
    
87.Vikram A, Jena G, Ramarao P. Insulin-resistance and benign prostatic hyperplasia: the connection. Eur J Pharmacol 2010; 641: 75-81.  Back to cited text no. 87
    
88.Weidner W, Krause W, Ludwig M. Relevance of male accessory gland infection for subsequent fertility with special focus on prostatitis. Hum Reprod Update 1999; 5: 421-32.  Back to cited text no. 88
    
89.Krause W. Male accessory gland infection. Andrologia 2008; 40: 113-6.  Back to cited text no. 89
[PUBMED]    
90.Rusz A, Pilatz A, Wagenlehner F, Linn T, Diemer T, et al. Influence of urogenital infections and inflammation on semen quality and male fertility. World J Urol 2012; 30: 23-30.  Back to cited text no. 90
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4]
 
 
    Tables

  [Table 1], [Table 2], [Table 3]


This article has been cited by
1 Management of male accessory gland inflammations: A response to Haidl et al.
Rossella Cannarella,Rosita A. Condorelli,Laura M. Mongioi’,Filippo Giacone,Aldo E. Calogero,Sandro La Vignera
Andrologia. 2019; : e13261
[Pubmed] | [DOI]
2 Testosterone and Benign Prostatic Hyperplasia
Giulia Rastrelli,Linda Vignozzi,Giovanni Corona,Mario Maggi
Sexual Medicine Reviews. 2019;
[Pubmed] | [DOI]
3 Best practice in the management of storage symptoms in male lower urinary tract symptoms: a review of the evidence base
Mauro Gacci,Arcangelo Sebastianelli,Pietro Spatafora,Giovanni Corona,Sergio Serni,Dirk De Ridder,Stavros Gravas,Paul Abrams
Therapeutic Advances in Urology. 2018; 10(2): 79
[Pubmed] | [DOI]
4 Epidemiology and risk factors of lower urinary tract symptoms/benign prostatic hyperplasia and erectile dysfunction
Aldo E. Calogero,Giovanni Burgio,Rosita A. Condorelli,Rossella Cannarella,Sandro La Vignera
The Aging Male. 2018; : 1
[Pubmed] | [DOI]
5 Epididymal more than testicular abnormalities are associated with the occurrence of antisperm antibodies as evaluated by the MAR test
F Lotti,E Baldi,G Corona,F Lombardo,E Maseroli,S Degl’Innocenti,L Bartoli,M Maggi
Human Reproduction. 2018; 33(8): 1417
[Pubmed] | [DOI]
6 Metabolic syndrome and the seminal cytokine network in morbidly obese males
A. Pilatz,C. Hudemann,J. Wolf,I. Halefeld,A. Paradowska-Dogan,H.-C. Schuppe,H. Hossain,Q. Jiang,D. Schultheiss,H. Renz,W. Weidner,F. Wagenlehner,T. Linn
Andrology. 2017; 5(1): 23
[Pubmed] | [DOI]
7 Association between metabolic syndrome and intravesical prostatic protrusion in patients with benign prostatic enlargement and lower urinary tract symptoms (MIPS Study)
Giorgio I. Russo,Federica Regis,Pietro Spatafora,Jacopo Frizzi,Daniele Urzì,Sebastiano Cimino,Sergio Serni,Marco Carini,Mauro Gacci,Giuseppe Morgia
BJU International. 2017;
[Pubmed] | [DOI]
8 Chronic prostatitis and its detrimental impact on sperm parameters: a systematic review and meta-analysis
R. A. Condorelli,G. Ivan Russo,A. E. Calogero,G. Morgia,S. La Vignera
Journal of Endocrinological Investigation. 2017;
[Pubmed] | [DOI]
9 Metabolic syndrome and benign prostatic hyperplasia: An update
Ho-Yin Ngai,Kar-Kei Steffi Yuen,Chi-Man Ng,Cheung-Hing Cheng,Sau-Kwan Peggy Chu
Asian Journal of Urology. 2017;
[Pubmed] | [DOI]
10 Benign prostatic enlargement can be influenced by metabolic profile: results of a multicenter prospective study
Mauro Gacci,Arcangelo Sebastianelli,Matteo Salvi,Cosimo De Nunzio,Linda Vignozzi,Giovanni Corona,Tommaso Jaeger,Tommaso Chini,Giorgio Ivan Russo,Mario Maggi,Giuseppe Morgia,Andrea Tubaro,Marco Carini,Sergio Serni
BMC Urology. 2017; 17(1)
[Pubmed] | [DOI]
11 Prostatic Calculi: Do They Matter?
Jun-Jie Cao,Wei Huang,Hong-Shen Wu,Min Cao,Yan Zhang,Xiao-Dong Jin
Sexual Medicine Reviews. 2017;
[Pubmed] | [DOI]
12 Male Lower Urinary Tract Symptoms and Cardiovascular Events: A Systematic Review and Meta-analysis
Mauro Gacci,Giovanni Corona,Arcangelo Sebastianelli,Sergio Serni,Cosimo De Nunzio,Mario Maggi,Linda Vignozzi,Giacomo Novara,Kevin T. McVary,Steven A. Kaplan,Stavros Gravas,Christopher Chapple
European Urology. 2016;
[Pubmed] | [DOI]
13 Lower urinary tract symptoms, benign prostatic hyperplasia and metabolic syndrome
Linda Vignozzi,Mauro Gacci,Mario Maggi
Nature Reviews Urology. 2016; 13(2): 108
[Pubmed] | [DOI]
14 Metabolic syndrome in white European men presenting for primary coupleæs infertility: investigation of the clinical and reproductive burden
E. Ventimiglia,P. Capogrosso,M. Colicchia,L. Boeri,A. Serino,G. Castagna,M. C. Clementi,G. La Croce,C. Regina,M. Bianchi,V. Mirone,R. Damiano,F. Montorsi,A. Salonia
Andrology. 2016;
[Pubmed] | [DOI]
15 Metabolic syndrome is associated with increased seminal inflammatory cytokines and reproductive dysfunction in a case-controlled male cohort
Kristian Leisegang,Patrick J. D. Bouic,Ralf R. Henkel
American Journal of Reproductive Immunology. 2016;
[Pubmed] | [DOI]
16 Is thyroid hormones evaluation of clinical value in the work-up of males of infertile couples?
F. Lotti,E. Maseroli,N. Fralassi,S. DeglæInnocenti,L. Boni,E. Baldi,M. Maggi
Human Reproduction. 2016; : dev338
[Pubmed] | [DOI]
17 Semen quality impairment is associated with sexual dysfunction according to its severity
F. Lotti,G. Corona,G. Castellini,E. Maseroli,M.G. Fino,M. Cozzolino,M. Maggi
Human Reproduction. 2016; 31(12): 2668
[Pubmed] | [DOI]
18 Male Lower Urinary Tract Symptoms Reveal and Predict Important Cardiovascular Disease
Mikkel Fode,Christian Gratzke,Jens Sønksen
European Urology. 2016; 70(5): 797
[Pubmed] | [DOI]
19 Metabolic syndrome, inflammation and lower urinary tract symptoms: possible translational links
Q He,Z Wang,G Liu,F Daneshgari,G T MacLennan,S Gupta
Prostate Cancer and Prostatic Disease. 2015;
[Pubmed] | [DOI]
20 Metabolic syndrome and benign prostatic hyperplasia: association or coincidence?
Aleksandra Ryl,Iwona Rotter,Tomasz Miazgowski,Marcin Slojewski,Barbara Dolegowska,Anna Lubkowska,Maria Laszczynska
Diabetology & Metabolic Syndrome. 2015; 7(1)
[Pubmed] | [DOI]
21 Current smoking is associated with lower seminal vesicles and ejaculate volume, despite higher testosterone levels, in male subjects of infertile couples
F. Lotti,G. Corona,P. Vitale,E. Maseroli,M. Rossi,M. G. Fino,M. Maggi
Human Reproduction. 2015;
[Pubmed] | [DOI]
22 Ultrasound of the male genital tract in relation to male reproductive health
F. Lotti,M. Maggi
Human Reproduction Update. 2014;
[Pubmed] | [DOI]



 

Top
 
 
  Search
 
Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

 
  In this article
Abstract
Introduction
Materials and Me...
Results
Discussion
Conclusions
Author Contributions
Competing Interests
References
Article Figures
Article Tables

 Article Access Statistics
    Viewed2995    
    Printed57    
    Emailed0    
    PDF Downloaded421    
    Comments [Add]    
    Cited by others 22    

Recommend this journal


[TAG2]
[TAG3]
[TAG4]